Downregulation of Src-Kinase and Glutamate-Receptor Phosphorylation after Traumatic Brain Injury

Abstract

Supplementary Figure I:Figure I. Western blot and quantitative analysis of NR1, NR2A, NR2B and phospho-NR2B Y1472 proteins in rat contralateral cortex after TBI. Neocortical tissues were obtained from the contralateral regions of sham-operated control rats and rats subjected to TBI followed by 0.5, 4 and 24 h recovery. Equal amounts of proteins from each sample in homogenate (Homo) and synaptosomal fractions (P2) were subjected to SDS-PAGE and immunoblotted with antibodies against NR1, NR2A, NR2B, phospho-NR2B Y1472, and β-actin respectively (A: NR1, B: NR2A, C: NR2B, D: phospho-NR2B Y1472). The changes of subunits NR1, NR2A, NR2B were normalized by β-actin, and phosphorylation level of NR2B Y1472 was normalized by total-NR2B. Data are

Glutamate and GABA Imbalance Following Traumatic Brain Injury

“…In rodents following FPI total NR2B protein and phosphorylation are down-regulated leading to destabilization of the NMDA receptor [62]. In addition to cumulative receptor downregulation, lateral FPI leads to a decrease in NR2A and increase in NR2B subunits in cortical NMDAR.…”

A novel phosphorylation site of N-methyl- d -aspartate receptor GluN2B at S1284 is regulated by Cdk5 in neuronal ischemia

“…To date, numerous serine/threonine and tyrosine phosphorylation sites of GluN2B have been identified and characterized because of significant advances of mass spectrum methods (Munton et al, 2007;Trinidad et al, 2008;Zhang et al, 2013) and phosphorylation specific antibodies generated for putative phosphorylation sites (Park et al, 2013). However, there remain many potential phosphorylation sites within the GluN2B subunit to be defined functionally.…”

From Molecular Circuit Dysfunction to Disease

“…As in epilepsy, increasing glutamate transport using ceftriaxone can be protective following TBI (Goodrich and others 2013). Studies examining glutamate receptors suggest acute decreases in NMDA receptor expression (Park and others 2013). Studies examining AMPA receptors show more variability (Atkins and others 2006; Spaethling and others 2008).…”