Downregulation of <scp>miR</scp>‐100‐5p in cancer‐associated fibroblast‐derived exosomes facilitates lymphangiogenesis in esophageal squamous cell carcinoma

Chen, C.; Yang, Chenbo; Tian, Xiangyu; Liang, Yinghao; Wang, Shuaiyuan; Wang, Xiaoqian; Shou, Yuwei; Li, Hui; Xiao, Qiankun; Shu, Jiao; Sun, Menghong; Chen, K S

doi:10.1002/cam4.6078

Cited by 14 publications

(9 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, OCSCC cells co-cultured with CAF-EVs tended to form larger tumors in the xenograft-bearing mice and increased lymph node metastases in mice induced with an orthotopic xenograft model. The most recent report also suggested that a large number of CAFs were present in esophageal squamous cell carcinoma, leading to a poor prognosis, and CAF-derived EVs enhanced lymphangiogenesis in xenografts formed by esophageal SCC cells [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Cancer-associated fibroblast-derived extracellular vesicles promote lymph node metastases in oral cavity squamous cell carcinoma by encapsulating ITGB1 and BMI1

Lv,

Liu,

Mao

et al. 2024

BMC Cancer

View full text Add to dashboard Cite

Background Extracellular vesicles (EVs) have been revealed to facilitate the development of oral squamous cavity cell carcinoma (OCSCC), while its supporting role in lymph node metastases is under continuous investigation. This study aimed to examine the function of cancer-associated fibroblasts (CAF)-derived EVs (CAF-EVs) during lymph node metastasis in OCSCC and the mechanisms. Methods CAF were isolated from OCSCC tissues of patients, and CAF-EVs were extracted and identified. EdU, colony formation, wound healing, and Transwell assays were performed. The OCSCC cells before and after CAF-EVs treatment were injected into mice to probe the effects of CAF-EVs on tumor growth and lymph node metastasis, respectively. The effect of CAF-EVs treatment on transcriptome changes in OCSCC cells was analyzed. Clinical data of patients with OCSCC were analyzed to determine the prognostic significance of the selected genes. Finally, loss-of-function assays were conducted to corroborate the involvement of polycomb complex protein BMI-1 (BMI1) and integrin beta1 (ITGB1). Results CAF-EVs promoted the malignant behavior of OCSCC cells and accelerated tumor growth and lymph node metastasis in mice. CAF-EVs significantly increased the expression of BMI1 and ITGB1, and the expression of BMI1 and ITGB1 was negatively correlated with the overall survival and relapse-free survival of OCSCC patients. Knockdown of BMI1 or ITGB1 in OCSCC cells abated the promoting effects of CAF-EVs in vitro and in vivo. Conclusion CAF-EVs elicited the metastasis-promoting properties in OCSCC by elevating BMI1 and ITGB1, suggesting that BMI1 and ITGB1 could be potential biomarkers and therapeutic targets for OCSCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Cancer-associated fibroblast-derived extracellular vesicles promote lymph node metastases in oral cavity squamous cell carcinoma by encapsulating ITGB1 and BMI1

Lv,

Liu,

Mao

et al. 2024

BMC Cancer

View full text Add to dashboard Cite

show abstract

“…Lymphatic metastasis is another important method of tumor metastasis besides hematogenous metastasis. The reduction of CAF-derived exosomal miR-100-5p in esophageal squamous cell carcinoma (ESCC) may promote the growth, migration, and invasion of tumor-related lymphatic endothelial cells by increasing the expression of IGF1R/PI3K/AKT, ultimately leading to the development of lymphatic vessels ( Chen et al, 2023 ) ( Figure 2 ).…”

Section: Function Of Caf-derived Exosomal Mirna In Promoting Tumorige...mentioning

confidence: 99%

The hidden messengers: cancer associated fibroblasts—derived exosomal miRNAs as key regulators of cancer malignancy

Gou,

Li,

Liu

et al. 2024

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Cancer-associated fibroblasts (CAFs), a class of stromal cells in the tumor microenvironment (TME), play a key role in controlling cancer cell invasion and metastasis, immune evasion, angiogenesis, and resistance to chemotherapy. CAFs mediate their activities by secreting soluble chemicals, releasing exosomes, and altering the extracellular matrix (ECM). Exosomes contain various biomolecules, such as nucleic acids, lipids, and proteins. microRNA (miRNA), a 22–26 nucleotide non-coding RNA, can regulate the cellular transcription processes. Studies have shown that miRNA-loaded exosomes secreted by CAFs engage in various regulatory communication networks with other TME constituents. This study focused on the roles of CAF-derived exosomal miRNAs in generating cancer malignant characteristics, including immune modulation, tumor growth, migration and invasion, epithelial-mesenchymal transition (EMT), and treatment resistance. This study thoroughly examines miRNA’s dual regulatory roles in promoting and suppressing cancer. Thus, changes in the CAF-derived exosomal miRNAs can be used as biomarkers for the diagnosis and prognosis of patients, and their specificity can be used to develop newer therapies. This review also discusses the pressing problems that require immediate attention, aiming to inspire researchers to explore more novel avenues in this field.

show abstract

“…demonstrated that CAFs-derived exosomes might accelerate cell proliferation and regulate apoptosis by RIG-I/IFN-b signaling and influence the chemosensitivity of ESCC to DDP, and that targeted inhibition of the RIG-I/IFN-b signaling axis in the exosomes of CAFs maybe an underlying therapeutic tactics for ESCC. Furthermore, the presence of CAFs and micro-lymphatic vessels in ESCC was associated with a poor prognosis (176). Exosomes derived from CAFs promote invasion, proliferation, migration, and tube formation of tumor-associated lymphatic endothelial cells (TLECs), while miR-100-5p exerts the opposite effect.…”

Section: Crosstalk Between Escc Cells and Cafsmentioning

confidence: 99%

Intercellular crosstalk between cancer cells and cancer-associated fibroblasts via exosomes in gastrointestinal tumors

Cao,

Ouyang

2024

Front. Oncol.

View full text Add to dashboard Cite

Gastrointestinal (GI) tumors are a significant global health threat, with high rates of morbidity and mortality. Exosomes contain various biologically active molecules like nucleic acids, proteins, and lipids and can serve as messengers for intercellular communication. They play critical roles in the exchange of information between tumor cells and the tumor microenvironment (TME). The TME consists of mesenchymal cells and components of the extracellular matrix (ECM), with fibroblasts being the most abundant cell type in the tumor mesenchyme. Cancer-associated fibroblasts (CAFs) are derived from normal fibroblasts and mesenchymal stem cells that are activated in the TME. CAFs can secrete exosomes to modulate cell proliferation, invasion, migration, drug resistance, and other biological processes in tumors. Additionally, tumor cells can manipulate the function and behavior of fibroblasts through direct cell-cell interactions. This review provides a summary of the intercellular crosstalk between GI tumor cells and CAFs through exosomes, along with potential underlying mechanisms.

show abstract

Downregulation of miR‐100‐5p in cancer‐associated fibroblast‐derived exosomes facilitates lymphangiogenesis in esophageal squamous cell carcinoma

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 14 publications

References 56 publications

Cancer-associated fibroblast-derived extracellular vesicles promote lymph node metastases in oral cavity squamous cell carcinoma by encapsulating ITGB1 and BMI1

Cancer-associated fibroblast-derived extracellular vesicles promote lymph node metastases in oral cavity squamous cell carcinoma by encapsulating ITGB1 and BMI1

The hidden messengers: cancer associated fibroblasts—derived exosomal miRNAs as key regulators of cancer malignancy

Intercellular crosstalk between cancer cells and cancer-associated fibroblasts via exosomes in gastrointestinal tumors

Contact Info

Product

Resources

About