Downregulation of RIP140 in triple‑negative breast cancer inhibits the growth and proliferation of cancer cells

Abstract

The aim of the present study was to investigate the expression of receptor interacting protein 140 (RIP140) in triple-negative breast cancer (TNBC), and its association with the prognosis of patients with TNBC. A total of 179 patients with breast cancer were included in this study, with 41 cases of TNBC and 138 cases of non-TNBC. Immunohistochemical staining and western blotting were used to detect the protein expression of RIP140 in the cancerous and paracancerous tissues, revealing that expression of RIP140 was increased in TNBC tissues compared with non-TNBC tissues. High expression of RIP140 in breast cancer tissue was associated with a poorer survival time than low RIP140 expression. Using lentiviral transfection to downregulate RIP140 expression in MDA-MB-231 cells, the effects of RIP140 on the growth and proliferation of breast cancer cells was analyzed using subcutaneous tumors in BALB/c nude mice. Immunohistochemical staining using a Ki-67 antibody in subcutaneous tumor tissue was used to assess the proliferation of MDA-MB-231 cells. The short hairpin RNA-mediated downregulation of RIP140 in MDA-MB-231 cells suppressed the growth and the proliferation of subcutaneous tumors in BALB/c nude mice. Downregulation of RIP140 in breast cancer cells may therefore inhibit the growth and the proliferation of these cells, and may provide a therapeutic target for TNBC.

RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53

“…We, and other groups, have also reported that RIP140 plays crucial roles in tumorigenesis, promoting (Aziz et al, 2015;Rosell et al, 2014;Yu et al, 2018) or impairing breast tumor cell proliferation (Docquier et al, 2010;Jalaguier et al, 2017). In colorectal cancers, RIP140…”

“…Moreover, RIP140 acts as a tumor suppressor, high expression of RIP140 correlating with good prognosis of colorectal cancer patients (Lapierre et al, 2014). In breast cancer, we, and other groups, have reported that RIP140 promotes (Aziz et al, 2015;Rosell et al, 2014;Yu et al, 2018) or impairs mammary tumor cell proliferation (Docquier et al, 2010;Jalaguier et al, 2017). Whether RIP140 is involved in the glucose metabolism of cancer cells and, if so, whether this directly affects tumor proliferation is still unknown.…”

The transcription factor RIP140 regulates interferon γ signaling in breast cancer

“…Conversely, GBP1 was described as a tumor suppressor in colon cancer [33]. These observations remind of RIP140 which has a protective role in colon [34] or triple negative breast cancer [13,35] whereas a high expression is associated with stomach cancer [36] as well as a shorter overall survival of cervical cancer patients [37]. GBP1 and RIP140 are a good illustration of tissue specificity of gene activity.…”

RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53

“…Moreover, RIP140 acts as a tumor suppressor, high expression of RIP140 correlating with good prognosis of colorectal cancer patients [24,25]. In breast cancer, we, and other groups, have reported that RIP140 promotes [26][27][28] or impairs mammary tumor cell proliferation [29,30]. Whether RIP140 is involved in the glucose metabolism of cancer cells and, if so, whether this directly affects tumor proliferation is still unknown.…”