Downregulation of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) is associated with enhanced expression of matrix metalloproteinases and cholangiocarcinoma metastases

Namwat, Nisana; Puetkasichonpasutha, Janpen; Loilome, Watcharin; Yongvanit, Puangrat; Techasen, Anchalee; Puapairoj, Anucha; Sripa, Banchob; Tassaneeyakul, Wichittra; Khuntikeo, Narong; Wongkham, Sopit

doi:10.1007/s00535-010-0345-y

Cited by 44 publications

(35 citation statements)

References 34 publications

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“…Similarly, our results showed a negative correlation between RECK and MMP-2 protein expression. In HUVECs, specific inhibition of MMP-2 significantly antagonized the effect of RECK depletion on β1-integrin signaling, cell proliferation, and tube elongation (Namwat et al, 2011). Moreover, RECK-mediated suppression of MMP-9 promoter activity requires 12-O-tetradecanoylphorbol-13-acetate-responsive element (TRE) and KB sites.…”

Section: Discussionmentioning

confidence: 99%

“…Hypermethylation of RECK promoter is also a common event in human ESCC, which occurs concurrently in tumor-adjacent normal mucosa and is correlated with poor prognosis in ESCC patients (Long NK et al, 2008). RECK displays as a metastasis suppressor and up-regulation of RECK expression could provide a potential therapy to improve the prognosis (Namwat et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Expression of MiR200a, miR93, Metastasis-related Gene RECK and MMP2/MMP9 in Human Cervical Carcinoma - Relationship with Prognosis

Wang

Wang²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of MiR200a, miR93, Metastasis-related Gene RECK and MMP2/MMP9 in Human Cervical Carcinoma - Relationship with Prognosis

Wang

Wang²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…In addition, the expressions of RECK and MMPs are inversely correlated [36]. Low RECK expression is strongly associated with high expression of MMP-2 and MMP-9 in different types of cancers [29,34,37]. RECK is considered to be a tumor and metastasis suppressor gene [32,33,34].…”

Section: Reversion-inducing Cysteine-rich Protein With Kazal Motifmentioning

confidence: 99%

HDAC Inhibitors and RECK Modulate Endoplasmic Reticulum Stress in Tumor Cells

Chen

Tsai

Tseng

2017

IJMS

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In the tumor microenvironment hypoxia and nutrient deprived states can induce endoplasmic reticulum (ER) stress. If ER stress is not relieved, the tumor cells may become apoptotic. Therefore, targeting ER homeostasis is a potential strategy for cancer treatment. Various chemotherapeutic agents including histone deacetylase (HDAC) inhibitors can induce ER stress to cause cell death in cancers. Some HDAC inhibitors can prevent HDAC from binding to the specificity protein 1-binding site of the promoter of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) and up-regulate RECK expression. Up-regulation of RECK expression by HDAC inhibitors has been observed in various cancer types. RECK is a tumor and metastasis suppressor gene and is critical for regulating tumor cell invasiveness and metastasis. RECK also modulates ER stress via binding to and sequestering glucose-regulated protein 78 protein, so that the transmembrane sensors, such as protein kinase RNA-like ER kinase are released to activate eukaryotic translational initiation factor 2α phosphorylation and enhance ER stress. Therefore, HDAC inhibitors may directly induce ER stress or indirectly induce this stress by up-regulating RECK in cancer cells.

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“…The breast carcinoma patients whose tumors exhibited low levels of RECK had a poor 5-year RFS rate than those with high RECK expression 8. RECK displays as a metastasis suppressor in cholangiocarcinoma, and upregulation of RECK expression could provide a potential therapy to improve the prognosis 9.…”

Section: Introductionmentioning

confidence: 99%

The Silencing of RECK Gene is Associated with Promoter Hypermethylation and Poor Survival in Hepatocellular Carcinoma

Zhang¹,

Yang²,

Zhang³

et al. 2012

Int. J. Biol. Sci.

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Background: To evaluate the promoter methylation status of RECK gene and mRNA expression in patients with hepatocellular carcinoma (HCC).Methods: We analyzed RECK methylation by MSP, and RECK mRNA by real-time PCR in 74 HCC. The liver cell lines (7721, Chang and Hep-G2) were treated with 5-Aza-CdR and TSA.Results: RECK mRNA were lower in HCC tissues (Mean -∆Ct = -3.29) than that in Non-Hcc tissues (Mean -∆Ct = -2.42). Expression of RECK was elevated in only 24 (32.43%) of the 74 HCC patients but decreased (-∆∆Ct<0) in 50 (67.57%) of the patients. RECK promoter was hypermethylated in 55.4% (41/74) of HCCs, and in only 17.6% (13/74) of Non-Hcc samples. RECK mRNA were lower in HCC patients with hypermethylation (∆MI>=0.5) (Mean -∆∆Ct = -1.75) than those with demethylation (∆MI<0.5) (Mean -∆∆Ct = 0.05), and there is a decreased tendency for RECK mRNA in HCC patients with promoter hypermethylation (p = 0.002). There was a significantly correlation found between RECK mRNA and poor survival after surgery. After treated by 5-Aza-CdR and TSA, we found that RECK mRNA induced different changes in 7721, Chang and Hep-G2 cells. And RECK demethylation also induced by epigenetic inhibitors.Conclusion: The results suggested that the hypermethylation may lead to promoter silencing of RECK mRNA and associated with poor survival in HCC.

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Downregulation of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) is associated with enhanced expression of matrix metalloproteinases and cholangiocarcinoma metastases

Abstract: RECK functions as a metastasis suppressor in CCA; upregulation of RECK expression could provide a potential therapy to improve the prognosis of this type of cancer.

Cited by 44 publications

References 34 publications

Expression of MiR200a, miR93, Metastasis-related Gene RECK and MMP2/MMP9 in Human Cervical Carcinoma - Relationship with Prognosis

Expression of MiR200a, miR93, Metastasis-related Gene RECK and MMP2/MMP9 in Human Cervical Carcinoma - Relationship with Prognosis

HDAC Inhibitors and RECK Modulate Endoplasmic Reticulum Stress in Tumor Cells

The Silencing of RECK Gene is Associated with Promoter Hypermethylation and Poor Survival in Hepatocellular Carcinoma

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