Downregulation of pigment epithelium-derived factor in condyloma acuminatum

Abstract

Objectives: To investigate pigment epithelium-derived factor (PEDF) mRNA and protein levels in condyloma acuminatum, and their relationship with angiogenesis and keratinocyte proliferation. Methods: Lesions from male patients with condyloma acuminatum and skin from healthy male (control) subjects were collected. Levels of PEDF protein and its corresponding mRNA (SERPINF1) were determined via Western blotting and reverse transcription-polymerase chain reaction, respectively. Immunohistochemical staining for Ki-67 and CD34 was performed to calculate keratinocyte proliferation index (PI) and microvessel density (MVD), respectively. Results: Levels of both PEDF protein and SERPINF1 mRNA were significantly lower in lesions from patients with condyloma acuminatum (n ¼ 30) than in skin from healthy control subjects (n ¼ 30). There were significant negative correlations between PEDF levels and both PI and MVD. Conclusions: The reduction in PEDF levels in condyloma acuminatum was associated with an increase in angiogenesis and cell proliferation. PEDF may be involved in the pathogenesis of condyloma acuminatum.

Development of secretome-based strategies to improve cell culture protocols in tissue engineering

“…Among these common proteins, it has been demonstrated that serpin E1 is able to stimulate keratinocyte migration during cutaneous injury repair 39 , while TIMP-1 and thrombospondin-1 have been linked to cell differentiation and migration, acting as growth factors 40 , 41 . In addition, PTX3 is reportedly involved in cell proliferation in prostate cells and other cell types 42 , 43 , and serpin F1 was shown to participate in the proliferation of human skin cells 44 . The presence of these relevant proteins in all three secretome studied here supports their use in skin tissue engineering protocols.…”

The Various Roles of PEDF in Cancer

“…PEDF is downregulated in condyloma acuminatum due to angiogenesis augmentation and cell proliferation. It has been found that its corresponding mRNA levels are also lower than normal in such lesions [32].…”

Harnessing Lignocellulosic Waste‐Derived Carbon Materials for Green Electrochemical Applications

“…Despite the significant importance of soil for life, a report from the European Union (EU) in 2023 asserts that approximately 60-70 % of the EU's soil is deemed unhealthy. [1] This contamination can be attributed to various anthropogenic factors, including the use of herbicides to control weeds and woody vegetation, [2] eutrophication resulting from excessive levels of N and P, [3] the presence of radioactive elements such as Cs, Am, Pu or Sr from combat borehole surface runoff, [4] the occurrence of microplastics, [5] leachate generated by the accumulation of urban solid waste [6] and the presence of heavy metals following the exploitation of a mining site. [7] Furthermore, the current poor state of water resources and their critical situation in many countries is also well-known.…”