Downregulation of N-cadherin Expression Inhibits Invasiveness, Arrests Cell Cycle and Induces Cell Apoptosis in Esophageal Squamous Cell Carcinoma

Li, Ké; He, Wei; Lin, Na; Wang, Xin; Fan, Qi-Wen

doi:10.3109/07357900903476745

Cited by 19 publications

(14 citation statements)

References 30 publications

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“…Moreover, the expressions of MMp-9 was also down-regulated in escc cell line ec9706 after transfection with n-cadherin shrnA (26). Additionally, the knockdown of n-cadherin in ec9706 could arrest cell cycle at g0/g1 phase, induce cell apoptosis, and inhibit tumor formation in vivo (27). It is known that growth factor receptors regulate many aspects of cell behavior, including cell motility and invasion (28).…”

Section: Discussionmentioning

confidence: 99%

CDH12 promotes the invasion of salivary adenoid cystic carcinoma

Wang

She²,

Su³

et al. 2011

Oncol Rep

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Abstract. cadherins are found in almost all living organisms. In addition to their role in the formation and maintenance of normal tissue architecture, cadherins seem to play a crucial role in the cell-cell interactions of cancer cells in tumorigenesis, invasion and metastasis. the aim of the present study was to identify the role of cDH12 in the invasion and metastasis of salivary adenoid cystic carcinoma (sAcc). real-time pcr results showed that cDH12 is abnormally expressed in the highly metastatic sAcc cell line Acc-M, compared to Acc-2, a sAcc cell line with low metastatic ability. cDH12 expression was significantly higher in clinical samples with metastasis and recurrence than in those without metastasis and recurrence (p<0.05), as demonstrated by immunohistochemical analysis. overexpression of the cDH12 protein in Acc-M cells infected with an adenovirus vector containing cDH12 enhanced the invasive and migratory ability of Acc-M cells in vitro compared to Acc-M cells infected with empty vector. likewise, knockdown of cDH12 by small interfering RNA efficiently inhibited the invasion and migration of Acc-M cells in vitro. these results indicate that cDH12 may play an important role in the invasion and metastasis of sAcc.

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Section: Discussionmentioning

confidence: 99%

CDH12 promotes the invasion of salivary adenoid cystic carcinoma

Wang

She²,

Su³

et al. 2011

Oncol Rep

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“…Cell cycle was performed using flow cytometry according to previous published methods (13). Briefly, 1x10 6 of untreated and transfected MCF-7 cells were harvested and washed in PBS, then fixed in 70% cold ethanol for 30 min at 4˚C.…”

Section: Methodsmentioning

confidence: 99%

Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7

Liu²,

Lv³

et al. 2011

Oncol Rep

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Abstract.It is well documented that B cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1), widely overexpressed in the vast majority of malignancies, plays an essential role in the occurrence and development of several different tumors. Here, we report Bmi-1 siRNA-mediated cell proliferation inhibition and cell apoptosis in vitro and in vivo in the human breast carcinoma cell line MCF-7. Our results demonstrated that Bmi-1 siRNA effectively down-regulated the expression of Bmi-1, inhibited cell proliferation in vitro and in vivo, evoked cell cycle arrest in the G 0 /G 1 phase and induced cell apoptosis in MCF-7 cells, coupled with decrease in cyclin D1, cyclin E, cdk2, bcl-2 and Ki-67 expression and Akt phosphorylation levels and an increase of p21 and bax expression and activities of caspase-3/-9. Taken together, our results suggest that Bmi-1 may be a potential molecular target for the therapy of breast carcinoma.

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“…Growth factors, cytokines, extracellular matrix components and transcription factors are involved in EMT induction in epithelial tumor cells (21,22). Results of recent studies (24)(25)(26)(27)(28)(29) suggest that EMT is associated with tumor invasion, metastasis and prognosis in ESCC. However, an association between SUVmax derived from FDG-PET/CT and EMT markers in ESCC remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

“…The expression of TIMP-1 is associated with tumor progression and a poor prognosis (28). N-cadherin is upregulated in invasive tumors, plays a key role in intercellular adhesion and is an important factor in ESCC tumor progression (29).…”

Section: Discussionmentioning

confidence: 99%

Maximum standardized uptake value in 18F-fluoro-2-deoxyglucose positron emission tomography is associated with advanced tumor factors in esophageal cancer

Kajiwara

Hiasa

Nishina

et al. 2014

Molecular and Clinical Oncology

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Abstract. Positron emission tomography/computed tomography (PET/CT) with 18 F-fluoro-2-deoxyglucose (FDG-PET/CT) has become established in cancer imaging, and derived maximum standardized uptake values (SUVmax) add functional information regarding cancer, including esophageal squamous cell carcinoma (ESCC). The aim of the present study was to determine the clinical significance and association of tumor progression using SUVmax derived from PET/CT images in patients with ESCC. In total, 101 patients with ESCC were assessed using FDG-PET/CT and the SUVmax was then compared with the clinical backgrounds and prognoses of the patients. Endoscopic ESCC biopsy specimens were obtained in order to analyze mRNA expression relative to tumor progression. The results showed that values for SUVmax were significantly higher in patients with tumor progression factors, particularly those with lymph node metastasis. Analysis of receiver operating characteristics curves revealed an optimum SUVmax cut-off value of 10.26 for node-positive disease. Patients with SUVmax ≥10.26 had gene alterations with epithelial-mesenchymal transition (EMT) and significantly worse overall survival (P=0.0012). A higher SUVmax in patients with ESCC was associated with lymph node metastasis and a poorer prognosis. Thus, the SUVmax may reflect the potential of EMT in patients with ESCC. IntroductionEsophageal cancer is an extremely lethal gastrointestinal neoplasm that leads to >300,000 mortalities worldwide annually (1). The reason for the poor prognosis is that >50% of patients already have unresectable or metastatic disease when they are diagnosed with esophageal cancer (2). A precise evaluation of the prognosis or overall survival (OS) of patients with esophageal cancer is essential for selecting appropriate treatment. Positron emission tomography/computed tomography (PET/CT) has become established in cancer imaging, and is useful for stratification during the primary staging of esophageal cancer by anatomical factors, such as tumor depth, invasion, lymph node metastasis and distant metastasis (3-10). However, 18 F-fluoro-2-deoxyglucose (FDG)-PET may be used to evaluate functional factors associated with tumor activity, which depends on glucose metabolism.It has been previously reported that the maximum standardized uptake value (SUVmax) in patients with certain types of cancer significantly correlates with survival, prognosis and recurrence (11,12). Although the clinical significance of SUVmax in esophageal cancer has been reported (13,14), the progression of esophageal cancer in specimens of esophageal squamous cell carcinoma (ESCC) using gene analysis has not yet been assessed. Therefore we investigated whether the

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Downregulation of N-cadherin Expression Inhibits Invasiveness, Arrests Cell Cycle and Induces Cell Apoptosis in Esophageal Squamous Cell Carcinoma

Cited by 19 publications

References 30 publications

CDH12 promotes the invasion of salivary adenoid cystic carcinoma

CDH12 promotes the invasion of salivary adenoid cystic carcinoma

Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7

Maximum standardized uptake value in 18F-fluoro-2-deoxyglucose positron emission tomography is associated with advanced tumor factors in esophageal cancer

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