Downregulation of MSP58 suppresses cell proliferation in neuroblastoma cell lines

Wu, Linquan; Zg, Zhang; Hz, Qin; Zhang, J; Gd, Gao; Lin, Weili; Wang, J

doi:10.1097/wnr.0b013e328359566e

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…MSP58si markedly reduced the proliferation and promoted the apoptosis of HepG2 and Huh7 cells and the Kaplan-Meier survival analysis in patients with HCC demonstrated that the survival time of the patients with low MSP58 expression was significantly longer than those with high expression during the 5-year follow-up period [6]. It was found that MSP58 was highly expressed in neuroblastoma tumor samples and cell lines and MSP58 targeted shRNA lentivirus attenuated neuroblastoma cell proliferation [25]. Our previous study also showed that MSP58 play an important role in glioma cell lines biological progression [7] and we further demonstrated that MSP58 expression correlates with the WHO grade and overall survival of patients with glioma [8].…”

Section: Discussionmentioning

confidence: 97%

Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients

Lin

Dai²,

Chen³

et al. 2016

Cell Physiol Biochem

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Background/Aims: The nucleolar 58-kDa microspherule protein (MSP58) has important transcriptional regulation functions and plays a crucial role in the tumorigenesis and progression of cancers. 3'-deoxy-3'-[¹⁸F]fluorothymidine (FLT) has emerged as a promising positron emission tomography (PET) tracer for evaluating tumor malignancy and cell proliferation. Methods: In the present study, the expression of MSP58 was evaluated by immunohistochemistry and the corresponding PET image was examined using FLT-PET in 55 patients with various grades of gliomas. Results: The immunoreactivity score (IRS) of MSP58 increased with tumor grade with grade IV gliomas exhibiting the highest expression and showed a highly significant positive correlation with the Ki-67 index (r = 0.65, P < 0.001). The IRS of MSP58 in the tumor showed a highly significant positive correlation with corresponding FLT uptake value (r = 0.61, P < 0.001). The correlation between MSP58 expression and glioma malignancy was also confirmed by immunofluorescence, RT-PCR and western blot analysis. FLT uptake value also exhibited a highly significant positive correlation with the Ki-67 index (r = 0.59, P < 0.001). Kaplan-Meier analysis revealed that MSP58 expression has a significant prognostic ability for the overall survival time similar to that found in the uptake value of FLT-PET. Conclusion: These results indicate that MSP58 plays an important role in cell proliferation and will be one of the potential candidates of molecular therapy targeting proliferation. FLT-PET might be used as an early measure of treatment response in the proliferation-targeted therapy.

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Section: Discussionmentioning

confidence: 97%

Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients

Lin

Dai²,

Chen³

et al. 2016

Cell Physiol Biochem

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“…In mouse, Mcrs1 also is expressed in the branchial arches and the otocyst (Gray et al, 2004). In mammalian cancer cells Mcrs1 has been implicated in proliferation (Shi et al, 2009; Wu et al, 2012; Zhong et al, 2013), and shown to function as a transcriptional repressor (Hsu et al, 2014). Mcrs1 also associates with the Fragile X mental retardation protein (FMRP) and may be involved in escorting silent ribonucleoparticles from the cell body of neurons to their distant synapses for translation (Davidovic et al, 2006).…”

Section: Six1 Co-factorsmentioning

confidence: 99%

Using Xenopus to discover new genes involved in branchiootorenal spectrum disorders

Moody

Neilson

Kenyon

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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Congenital hearing loss is an important clinical problem because, without early intervention, affected children do not properly acquire language and consequently have difficulties developing social skills. Although most newborns in the US are screened for hearing deficits, even earlier diagnosis can be made with prenatal genetic screening. Genetic screening that identifies the relevant mutated gene can also warn about potential congenital defects in organs not related to hearing. We will discuss efforts to identify new candidate genes that underlie the Branchiootorenal spectrum disorders in which affected children have hearing deficits and are also at risk for kidney defects. Mutations in two genes, SIX1 and EYA1, have been identified in about half of the patients tested. To uncover new candidate genes, we have used the aquatic animal model, Xenopus laevis, to identify genes that are part of the developmental genetic pathway of Six1 during otic and kidney development. We have already identified a large number of potential Six1 transcriptional targets and candidate co-factor proteins that are expressed at the right time and in the correct tissues to interact with Six1 during development. We discuss the advantages of using this system for gene discovery in a human congenital hearing loss syndrome.

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“…In addition to participating in mitotic spindle assembly and mTOR signaling, MCRS1 is an oncogene [ 14 , 15 ]. MCSR1 has been found to be overexpressed in neuroblastoma, lung cancer, gastric cancer, liver cancer, colorectal cancer, and kidney cancer, and is associated with increased tumor growth, invasion and metastasis [ 15 , 16 , 17 , 18 , 19 , 20 ]. In 2019, using two gastric cancer cell lines (BGC-823 and SGC-7901), Wang et al found that suppressing MCSR1 expression can inhibit tumor activity [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

MCRS1 Expression Regulates Tumor Activity and Affects Survival Probability of Patients with Gastric Cancer

et al. 2022

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Gastric cancer is the fifth most common cancer worldwide and the third most common cause of cancer-related deaths. Surgery remains the first-choice treatment. Chemotherapy is considered in the middle and advanced stages, but has limited success. Microspherule protein 1 (MCRS1, also known as MSP58) is a protein originally identified in the nucleus and cytoplasm that is involved in the cell cycle. High expression of MCRS1 increases tumor growth, invasiveness, and metastasis. The mechanistic relationships between MCSR1 and proliferation, apoptosis, angiogenesis, and epithelial–mesenchymal transition (EMT) remain to be elucidated. We clarified these relationships using immunostaining of tumor tissues and normal tissues from patients with gastric cancer. High MCRS1 expression in gastric cancer positively correlated with Ki-67, Caspase3, CD31, Fibronectin, pAKT, and pAMPK. The hazard ratio of high MCRS1 expression was 2.44 times that of low MCRS1 expression, negatively impacting patient survival.

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Downregulation of MSP58 suppresses cell proliferation in neuroblastoma cell lines

Cited by 8 publications

References 18 publications

Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients

Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients

Using Xenopus to discover new genes involved in branchiootorenal spectrum disorders

MCRS1 Expression Regulates Tumor Activity and Affects Survival Probability of Patients with Gastric Cancer

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