Downregulation of miR‐491‐5p promotes gastric cancer metastasis by regulating <scp>SNAIL</scp> and <scp>FGFR</scp>4

Takata, Yasuyuki; Wang, Li‐na; Li, Wei; Zuo, Qiang; Li, Mengmeng; Zou, Quanming; Xiao, Bin

doi:10.1111/cas.13583

Cited by 62 publications

(48 citation statements)

References 27 publications

(70 reference statements)

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“…In addition, high expression of the chemokine family of proteins, such as GRB2 and JAK3, involved in proliferation, migration, and invasion of cancers, was found (12,13). Moreover, FGFR4, reported to be associated with epithelialmesenchymal transition (EMT) and metastasis, was also shown to be significant in the PPI network (14,15). The up-regulated and down-regulated genes had complex interactions with other genes in the signaling network (Figure 4).…”

Section: Ppi Network and Module Analysismentioning

confidence: 95%

Chemo-resistant Gastric Cancer Associated Gene Expression Signature: Bioinformatics Analysis Based on Gene Expression Omnibus

Liu¹,

Jian²,

Yue³

et al. 2019

Anticancer Res

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Background/Aim: This study aimed to identify biomarkers for predicting the prognosis of advanced gastric cancer patients who received docetaxel, cisplatin, and S-1 (DCS). Materials and Methods: Gene expression profiles were obtained from the Gene Expression Omnibus database (GSE31811). Gene-Ontology-enrichment and KEGG-pathway analysis were used for evaluating the biological functions of differentially-expressed genes. Protein-protein interaction (PPI) network and Kaplan-Meier survival analyses were employed to assess the prognostic values of hub genes. Results: A total of 1,486 differentially expressed genes (DEGs) were identified, including 13 up-regulated and 1,473 down-regulated genes. KEGG pathways such as metabolic pathways, cell adhesion molecules (CAMs), PI3K-Akt signaling pathway and pathways in cancer were significantly represented. In the PPI network, the top ten hub genes ranked by degree were GNG7,

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Section: Ppi Network and Module Analysismentioning

confidence: 95%

Chemo-resistant Gastric Cancer Associated Gene Expression Signature: Bioinformatics Analysis Based on Gene Expression Omnibus

Liu¹,

Jian²,

Yue³

et al. 2019

Anticancer Res

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“…According to the search results of star-Base (http://starb ase.sysu.edu.cn), total of 23 miRNAs was screened out as downstream candidates of LBX2-AS1 (Additional file 3: Table S1). Among those candidates, miR-491-5p has been revealed to suppress gastric carcinogenesis by previous researches [17,18]. Therefore, we chose miR-491-5p to continue our study.…”

Section: Lbx2-as1 Functioned As a Cerna In Gc Cellsmentioning

confidence: 99%

LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703

Zhang

et al. 2020

Cancer Cell Int

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Background: The crucial role of long non-coding RNAs (lncRNAs) has been certified in human cancers. The lncRNAs with abnormal expressions could act as tumor inhibitors or oncogenes in the advancement of tumors. LBX2-AS1 was once reported to accelerate esophageal squamous cell carcinoma. Nonetheless, its function in gastric cancer (GC) remained a riddle. Methods: RT-qPCR was used to examine the expression of NFIC/LBX2-AS1/miR-491-5p/ZNF703 in GC cell lines. The functions of LBX2-AS1 in GC were appraised by colony formation, EdU, flow cytometry analysis, transwell and wound healing assays. Luciferase reporter, ChIP and RNA pull down assays were utilized to evaluate the interactions among genes. Results: LBX2-AS1 was up-regulated in GC cell lines. Knockdown of LBX2-AS1 repressed the proliferative, migratory, and invasive abilities of GC cells. Moreover, LBX2-AS1 was transcriptionally activated by NFIC. And LBX2-AS1 could bind with miR-491-5p. Besides, miR-491-5p depletion or ZNF703 upregulation could counteract the repressing effects of LBX2-AS1 silence on GC progression. Conclusion: In a word, LBX2-AS1 up-regulated by NFIC promoted GC progression via targeting miR-491-5p/ZNF703, implying LBX2-AS1 was an underlying treatment target for GC patients.

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“…Wang et al demonstrates that over-expression of miR-217 inhibits gastric cancer cell proliferation, invasion and promotes apoptosis via regulating GPC5, which acts as a potential therapeutic target [20]. Besides, other miRNAs, for instance, miR-376c-3p [21], miR-133b [22], miR-99b-5p and miR-203a-3p [23], miR-491-5p [24], miR-143 [25] are intensively involved in the regulation of the development and progression of gastric cancer. Thus miRNAs is closely associated with the occurrence and development of gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

MiR-450 inhibits cell proliferation, migration and invasion and induces apoptosis in gastric cancer via targeting CREB1

Zhao¹,

Zhang²,

Y³

et al. 2020

Preprint

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Background：Gastric cancer seriously affects human health and research on gastric cancer is increasing. In recent years, molecular targets are hot research topics. We aimed to explore the effects and mechanisms of miR-450 on the development and progression of gastric cancer.Methods：We used gain-of-function approaches to investigate the gastric cancer cell proliferation, apoptosis, migration and invasion, including, RT-qPCR, CCK-8, colony formation, flow cytometry, western blot, wound healing, transwell chamber, HE, TUNEL, dual luciferase reporter and tumor formation analysis.Results：We found that the expression levels of miR-450 were greatly decreased in gastric cancer cells and overexpression of miR-450 inhibited the gastric cancer cell proliferation, migration and invasion, while induced apoptosis in gastric cancer in vitro. Moreover, we demonstrated that ectopic expression of miR-450 inhibited tumor growth in vivo. At the molecular level, overexpression of miR-450 significantly increased the expression levels of apoptosis-associated proteins, including Caspase-3, Caspase-9 and BAX, while inhibited the expression level of Bcl-2. Mechanically, luciferase reporter experiment suggested that CREB1 had a negative correlation with miR-450 expression and knockdown of CREB1 alleviated gastric cancer. Furthermore, we also found that miR-450 inhibited the activation of AKT/GSK-3β signaling pathway to inhibit the progression of gastric cancer.Conclusions：miR-450 repressed gastric cancer cell proliferation, migration and invasion and induced apoptosis through targeting CREB1 by modulating AKT/GSK-3β signaling pathway, which may provide a new molecular target for the treatment of gastric cancer.

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Downregulation of miR‐491‐5p promotes gastric cancer metastasis by regulating SNAIL and FGFR4

Cited by 62 publications

References 27 publications

Chemo-resistant Gastric Cancer Associated Gene Expression Signature: Bioinformatics Analysis Based on Gene Expression Omnibus

Chemo-resistant Gastric Cancer Associated Gene Expression Signature: Bioinformatics Analysis Based on Gene Expression Omnibus

LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703

MiR-450 inhibits cell proliferation, migration and invasion and induces apoptosis in gastric cancer via targeting CREB1

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