Downregulation of miR-3940-5p promotes T-cell activity by targeting the cytokine receptor IL–2R gamma on human cutaneous T-cell lines

Wang, Yi; Wang, Keyu; Wang, Lihua; Zhang, Min

doi:10.1016/j.imbio.2016.07.008

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Nineteen of the downregulated miRNAs in chronic patients were found to be associated with carcinogenesis in various organs and tissues, such as miR-1207 [ 40 ], miR-3162-5p [ 41 , 42 ], miR-3196 [ 43 , 44 ], miR-371b-5p [ 45 ], miR-574-5p [ 46 , 47 ], miR-1225-5p [ 48 ], miR-4485 [ 49 ], miR-572 [ 50 ], miR-4299 [ 51 ], miR-3679-5p [ 52 ], miR-3940-5p [ 53 ], miR-638 [ 54 , 55 ], miR-1202 [ 56 ], miR-5787 [ 57 ], miR-1973 [ 58 ], miR-4532 [ 59 ], miR-1275 [ 60 ], miR-4728-5p [ 61 ], and miR-1915-3p [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

et al. 2018

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Brucellosis is a serious infectious disease that continues to be a significant cause of morbidity worldwide and across all ages. Despite early diagnosis and treatment, 10–30% of patients develop chronic brucellosis. Although there have been recent advances in our knowledge of Brucella virulence factors and hosts’ immune response to the infection, there is a lack of clear data regarding how the infection bypasses the immune system and becomes chronic. The present study investigated immunological factors and their roles in the transition of brucellosis from an acute to a chronic infection in CD4+ T cells. CD4+ T cells sorted from peripheral blood samples of patients with acute or chronic brucellosis and healthy controls using flow cytometry as well as more than 2000 miRNAs were screened using the GeneSpring GX (Agilent) 13.0 miRNA microarray software and were validated using reverse transcription polymerase chain reaction (RT-qPCR). Compared to acute cases, the expression levels of 28 miRNAs were significantly altered in chronic cases. Apart from one miRNA (miR-4649-3p), 27 miRNAs were not expressed in the acute cases (p <0.05, fold change> 2). According to KEGG pathway analysis, these miRNAs are involved in the regulation of target genes that were previously involved in the MAPK signalling pathway, regulation of the actin cytoskeleton, endocytosis, and protein processing in the endoplasmic reticulum. This indicates the potential role of these miRNAs in the development of chronic brucellosis. We suggest that these miRNAs can be used as markers to determine the transition of the disease into chronicity. This is the first study of miRNA expression that analyses human CD4+ T cells to clarify the mechanism of chronicity in brucellosis.

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Section: Discussionmentioning

confidence: 99%

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

et al. 2018

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“…The aberrant expression of miRNAs has been shown to be associated with various malignancies ( 14 - 16 ) and miRNAs can serve as non-invasive biomarkers for the prognosis of patients with GC ( 17 , 18 ). miR-30a has been shown to suppress Th17 differentiation and demyelination by targeting IL-21R ( 19 ), and integrated miRNA-mRNA profiling has identified miR-583 as a negative regulator of IL2Rγ ( 20 ); however, the inhibition of miR-3940-5p has been shown to facilitate T-cell activity by targeting IL2Rγ ( 21 ). Increasing evidence documents the potential role of long non-coding RNAs (lncRNAs) as miRNA sponges in human cancer, and lncRNAs may emerge as important players in the regulation of miRNAs and their target genes ( 22 , 23 ).…”

Section: Introductionmentioning

confidence: 99%

IL-21R functions as an oncogenic factor and is regulated by the lncRNA MALAT1/miR-125a-3p axis in gastric cancer

et al. 2018

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Interleukin-21 receptor (IL-21R) is involved in the immunological regulation of immune cells and tumor progression in multiple malignancies. However, the potential molecular mechanisms through which non-coding RNAs (ncRNAs) modulate IL-21R signaling in gastric cancer (GC) remain elusive. In this study, the expression of IL-21R was detected by RT-qPCR and western blot analysis in GC cell lines. The association between IL-21R expression and clinicopathological characteristics and the prognosis of patients with GC was analyzed by immunohistochemistry and Kaplan-Meier plotter analysis. The biological functions of IL-21R were analyzed by a series of in vitro and in vivo experiments, and its regulation by ncRNAs was predicted by bioinformatics analysis and confirmed by luciferase assays and rescue experiments. As a result, the expression of IL-21R was found to be significantly increased in GC cell lines and tissues as compared with normal tissues, and was associated with tumor size and lymphatic metastasis, acting as an independent prognostic factor of poor survival and recurrence in patients with GC. The knockdown of IL-21R markedly suppressed GC cell proliferation and invasion, and IL-21R expression was further validated to be negatively regulated by miR-125a-3p (miR-125a). The overexpression of IL-21R reversed the tumor suppressive effects of miR-125a in vitro and in vivo. Moreover, lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) acted as a sponge of miR-125a to modulate the IL-21R signaling pathway in GC cells and represented a risk factor for survival and recurrence in patients with GC. Taken together, the findings of this study reveal an oncogenic role for IL-21R in gastric tumorigenesis and verify that its activation is partly due to the dysregulation of the lncRNA MALAT1/miR-125a axis. These findings may provide a potential prognostic marker for patients with GC.

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“…A study suggested that increased miRNA, miR‐146a, miR‐191 and miR‐342‐3p may be involved in vitiligo development . As a support, another study claimed that there was a correlation between upregulated and downregulated miRNA and the immune imbalance of vitiligo patients . Although a large amount of differentially expressed miRNA have been discovered in vitiligo, only a few targets have been identified and the potential function in BP remains unknown .…”

Section: Introductionmentioning

confidence: 99%

Altered expression of four miRNA (miR‐1238‐3p, miR‐202‐3p, miR‐630 and miR‐766‐3p) and their potential targets in peripheral blood from vitiligo patients

Shang

2017

The Journal of Dermatology

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Vitiligo is an acquired skin disease with pigmentary disorder. Autoimmune destruction of melanocytes is thought to be major factor in the etiology of vitiligo. miRNA-based regulators of gene expression have been reported to play crucial roles in autoimmune disease. Therefore, we attempt to profile the miRNA expressions and predict their potential targets, assessing the biological functions of differentially expressed miRNA. Total RNA was extracted from peripheral blood of vitiligo (experimental group, n = 5) and non-vitiligo (control group, n = 5) age-matched patients. Samples were hybridized to a miRNA array. Box, scatter and principal component analysis plots were performed, followed by unsupervised hierarchical clustering analysis to classify the samples. Quantitative reverse transcription polymerase chain reaction (RT-PCR) was conducted for validation of microarray data. Three different databases, TargetScan, PITA and microRNA.org, were used to predict the potential target genes. Gene ontology (GO) annotation and pathway analysis were performed to assess the potential functions of predicted genes of identified miRNA. A total of 100 (29 upregulated and 71 downregulated) miRNA were filtered by volcano plot analysis. Four miRNA were validated by quantitative RT-PCR as significantly downregulated in the vitiligo group. The functions of predicted target genes associated with differentially expressed miRNA were assessed by GO analysis, showing that the GO term with most significantly enriched target genes was axon guidance, and that the axon guidance pathway was most significantly correlated with these miRNA. In conclusion, we identified four downregulated miRNA in vitiligo and assessed the potential functions of target genes related to these differentially expressed miRNA.

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Downregulation of miR-3940-5p promotes T-cell activity by targeting the cytokine receptor IL–2R gamma on human cutaneous T-cell lines

Cited by 9 publications

References 10 publications

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

The microRNA expression signature of CD4+ T cells in the transition of brucellosis into chronicity

IL-21R functions as an oncogenic factor and is regulated by the lncRNA MALAT1/miR-125a-3p axis in gastric cancer

Altered expression of four miRNA (miR‐1238‐3p, miR‐202‐3p, miR‐630 and miR‐766‐3p) and their potential targets in peripheral blood from vitiligo patients

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