Downregulation of miR-374b-5p promotes chemotherapeutic resistance in pancreatic cancer by upregulating multiple anti-apoptotic proteins

Sun, Di; Wang, Xu; Sui, Guoqing; Chen, Si; Yu, Miao; Zhang, Ping

doi:10.3892/ijo.2018.4315

Cited by 28 publications

(30 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the downregulated miR-144-3p was correlated to poor diseasefree survival. As for miR-369-3p and miR-374b-5p, they were dysregulated in various types of cancers, but no study has been conducted to explore their functions and mechanisms in HCC [56][57][58][59][60].…”

Section: Discussionmentioning

confidence: 99%

Clinical Value and Potential Mechanisms Exploration of GOLGA8B in Hepatocellular Carcinoma: A Comprehensive Investigation Based on Real-Time Quantitative Polymerase Chain Reaction, RNA-seq, Microarray and Immunohistochemistry

Ma¹,

Zhai²,

Wu³

et al. 2019

Preprint

View full text Add to dashboard Cite

Introduction Recent studies found that GOLGA8B plays an essential role in different cancers. However, the role GOLGA8B plays in the carcinogenesis and development of hepatocellular carcinoma (HCC) remains unclear. This study explores the clinical significance and prospective mechanisms of GOLGA8B in HCC. Materials and methods The expression of GOLGA8B was detected in tissues of HCC and non-HCC controls. A real-time quantitative polymerase chain reaction analysis was performed to evaluate the mRNA expression of GOLGA8B. RNA-sequencing data and microarray chip data were obtained for further analysis. The role GOLGA8B plays in patients with HCC was also evaluated. An immunohistochemistry (IHC) analysis was also performed to evaluate the protein expression of GOLGA8B. The different GOLGA8B expression resources, including mRNA and protein expression, were integrated by calculating standard mean difference (SMD) and summary of the receiver operator characteristic (sROC). Genes co-expressed GOLGA8B were predicted. Enrichment analyses including Gene ontology (GO) and biological pathway were performed to investigate the essential molecular mechanisms. Hub genes were screened out by a protein-protein interactions network. MicroRNAs which target GOLGA8B at a posttranscriptional level were also predicted. Results According to different resources, GOLGA8B manifested a higher expression in tissues of HCC than in non-HCC controls and exhibited clinical values for HCC. The RT-qPCR analysis revealed an increasing trend of GOLGA8B expression in HCC. GOLGA8B expresssion was significantly increased in RNA-sequencing and 7 of 13 microarray chip. IHC analysis also revealed significantly higher expression of GOLGA8B protein. Moreover, GOLGA8B expression was correlated with pathologic tumors and stages according to RNA-sequencing data and IHC analysis. The integrated SMD and sROC of different resources was 0.893 (P=0.004) and 0.79. A total 1303 co-expressed genes were gathered to perform enrichment analyses. The most significant biological pathways of co-expressed genes were spliceosome and mitogen-activated protein kinase signalling (MAPK). Four hub genes (SF3B1, HNRNPA2B1, HNRNPA1 and SRRM2) and five miRNAs (miR-369-3p, miR-203a, miR-374b-5p, miR-139-5p and miR-144-3p) were screened out. Conclusion GOLGA8B expression was increased in HCC and may serve as a novel target for HCC diagnosis and treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Clinical Value and Potential Mechanisms Exploration of GOLGA8B in Hepatocellular Carcinoma: A Comprehensive Investigation Based on Real-Time Quantitative Polymerase Chain Reaction, RNA-seq, Microarray and Immunohistochemistry

Ma¹,

Zhai²,

Wu³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…miR-374b downregulation has been linked to cancer aggressiveness and resistance to therapy in various cancers of the gastrointestinal tract, including CRC, pancreatic, and gastric cancer [77,78]. miR-374b downregulation has been linked to cancer aggressiveness and resistance to therapy in various cancers of the gastrointestinal tract, including CRC, pancreatic, and gastric cancer [77,78].…”

Section: Mirnas Modulation Of Nk Cellsmentioning

confidence: 99%

“…Unleashing the NK cells represent an essential aspect for boosting the antitumor immunity. miR-374b downregulation has been linked to cancer aggressiveness and resistance to therapy in various cancers of the gastrointestinal tract, including CRC, pancreatic, and gastric cancer [77,78]. The overexpression of miRNA-374b can halt the progression of hepatic tumors through downregulating the expression of PD-1 by cytokine-induced NK cells and restoring their antitumor activity [79].…”

Section: Mirnas Modulation Of Nk Cellsmentioning

confidence: 99%

Immunomodulatory MicroRNAs in cancer: targeting immune checkpoints and the tumor microenvironment

et al. 2019

View full text Add to dashboard Cite

Cancer immunotherapy represents a promising new era in cancer management due to the relatively high safety margins and selectivity, compared to the classical cancer chemotherapeutic agents. However, there is an imperative need to overcome tumor resistance in order to improve clinical outcomes and maximize the benefits of cancer immunotherapy. The interaction between the programmed cell death‐1 (PD‐1) receptor and its ligand PD‐L1 is a vital immune checkpoint that is often adopted by cancer cells to undergo immune evasion. PD‐1/PD‐L1 signaling is regulated at multiple levels through the crosstalk with other immune targets or relevant signaling partners involved in the cancer progression. Among the significant epigenetic players that are implicated in modulating the immune system are microRNAs (miRNAs). A complex system of these noncoding RNAs regulates the gene expression at the post‐transcriptional level and plays a significant role in the modulation of both innate and the adaptive immune systems. The expression profile of immune‐modulatory miRNAs might be useful as a predictive biomarker for the response and clinical outcomes in cancer immunotherapy. Therefore, in the current review, we highlighted the role of miRNAs in cancer immune evasion through a critical discussion of their impact on key immune checkpoints as well as the role of miRNAs in cancer progression and resistance.

show abstract

“…Molecular prognostic biomarkers are the basic components of precision medicine. Data mining and other biological analysis make it possible to predict the prognosis of tumors at the molecular level (3)(4)(5). Accurate clinical estimation using prognostic biomarkers helps determining optimal anti-cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Review of Web Servers and Bioinformatics Tools for Cancer Prognosis Analysis

Zheng

Zhang

et al. 2020

Front. Oncol.

View full text Add to dashboard Cite

Prognostic biomarkers are of great significance to predict the outcome of patients with cancer, to guide the clinical treatments, to elucidate tumorigenesis mechanisms, and offer the opportunity of identifying therapeutic targets. To screen and develop prognostic biomarkers, high throughput profiling methods including gene microarray and next-generation sequencing have been widely applied and shown great success. However, due to the lack of independent validation, only very few prognostic biomarkers have been applied for clinical practice. In order to cross-validate the reliability of potential prognostic biomarkers, some groups have collected the omics datasets (i.e., epigenetics/transcriptome/proteome) with relative follow-up data (such as OS/DSS/PFS) of clinical samples from different cohorts, and developed the easy-to-use online bioinformatics tools and web servers to assist the biomarker screening and validation. These tools and web servers provide great convenience for the development of prognostic biomarkers, for the study of molecular mechanisms of tumorigenesis and progression, and even for the discovery of important therapeutic targets. Aim to help researchers to get a quick learning and understand the function of these tools, the current review delves into the introduction of the usage, characteristics and algorithms of tools, and web servers, such as LOGpc, KM plotter, GEPIA, TCPA, OncoLnc, PrognoScan, MethSurv, SurvExpress, UALCAN, etc., and further help researchers to select more suitable tools for their own research. In addition, all the tools introduced in this review can be reached at http://bioinfo.henu.edu.cn/WebServiceList.html.

show abstract

Downregulation of miR-374b-5p promotes chemotherapeutic resistance in pancreatic cancer by upregulating multiple anti-apoptotic proteins

Cited by 28 publications

References 56 publications

Clinical Value and Potential Mechanisms Exploration of GOLGA8B in Hepatocellular Carcinoma: A Comprehensive Investigation Based on Real-Time Quantitative Polymerase Chain Reaction, RNA-seq, Microarray and Immunohistochemistry

Clinical Value and Potential Mechanisms Exploration of GOLGA8B in Hepatocellular Carcinoma: A Comprehensive Investigation Based on Real-Time Quantitative Polymerase Chain Reaction, RNA-seq, Microarray and Immunohistochemistry

Immunomodulatory MicroRNAs in cancer: targeting immune checkpoints and the tumor microenvironment

Comprehensive Review of Web Servers and Bioinformatics Tools for Cancer Prognosis Analysis

Contact Info

Product

Resources

About