Downregulation of microRNA-497 is associated with upregulation of synuclein γ in patients with osteosarcoma

Abstract

Abstract. The present study aimed to investigate the effects of microRNA (miRNA/miR)-497 expression levels on the expression levels of synuclein γ (SNCG) in serum samples, as well as osteosarcoma and lung-metastatic tissue samples, from patients with osteosarcoma. Between December 2010 and August 2013, fasting peripheral blood was collected from 36 patients with osteosarcoma for serum separation. In addition, osteosarcoma and lung metastatic tissues were resected from 15 osteosarcoma patients with lung metastasis by surgery. Bioinformatics was employed to predict the amount miRNA that binds to SNCG. Reverse transcription-quantitative polymerase chain reaction was used to determine the expression levels of SNCG and miR-497, and western blotting was performed to determine protein expression levels. It was observed that SNCG mRNA and protein expression levels were significantly upregulated in osteosarcoma tissues (P<0.01). Additionally, SNCG mRNA (P<0.01) and protein (P<0.05) expression levels were significantly upregulated in the blood of patients with osteosarcoma. SNCG mRNA and protein expression levels were also significantly upregulated in lung metastatic tissues (P<0.01). miR-497 was significantly downregulated in all three samples; therefore downregulation of miR-497 may lead to the occurrence, development and metastasis of osteosarcoma through the upregulation of SNCG mRNA. In summary, the upregulation of SNCG in blood, osteosarcoma tissue and lung metastatic tissue samples is associated with the dowregulation of miR-497, suggesting that miR-497 may be a potential marker and therapeutic target for the treatment of osteosarcoma.

SNCG promotes the progression and metastasis of high-grade serous ovarian cancer via targeting the PI3K/AKT signaling pathway

“…In our cohort, we revealed that the higher level of SNCG had a significant correlation with worse PFS and OS in HGSOC patients, which indicated its role in HGSOC as well as a novel prognostic marker of HGSOC aggression. Some studies have shown that SNCG could be detected in the circulation of patients with cancer [29,30]. Future studies are needed to evaluate the potential of serum SNCG levels as a biomarker for HGSOC patients.…”

The Role of miR-92b-3p in Notch Signaling and Monitoring of Oral Squamous Cell Carcinoma

“…Circulating miRNAs not only facilitate early cancer diagnosis, but also aid in monitoring tumor metastasis. Reports have found substantial downregulation of miR-497-5p expression in primary osteosarcoma tissues, metastases, and serum versus healthy controls [33,34]. The results from the CNUH cohort indicate that elevated miR-92b-3p expression was associated with increased tumor volume and lymph node metastasis in OSCC.…”

Διερεύνηση διαγνωστικών και προγνωστικών υπογραφών έκφρασης MicroRnas στα λιποσαρκώματα

“…Τέλος, το SNCG mRNA (πιθανά με ρόλο στην ενεργοποίηση του MAPK and Elk-1 μονοπατίου) και τα επίπεδα έκφρασης πρωτεΐνης του ήταν σημαντικά αυξημένα σε ιστούς οστεοσαρκώματος (Ρ <0,01), στο αίμα των ασθενών με οστεοσάρκωμα (Ρ <0.01) και σε ιστούς μεταστατικούς στον πνεύμονα (Ρ <0,01). Το miR-497 ήταν σημαντικά μειωμένο και στα τρία δείγματα.Ως εκ τούτου, μειωμένη ρύθμιση του miR-497 μπορεί να οδηγήσει στην ανάπτυξη και μετάσταση του οστεοσαρκώματος μέσω της αυξημένης ρύθμισης SNCG mRNA (γονίδιο-στόχος) [310].…”