Downregulation of intratumoral expression of miR-205, miR-200c and miR-125b in primary human cutaneous melanomas predicts shorter survival

Sánchez‐Sendra, Beatriz; Martínez-Ciarpaglini, Carolina; González-Muñoz, José F.; Murgui, Amelia; Terrádez, Liria; Monteagudo, Carlos

doi:10.1038/s41598-018-35317-3

Cited by 27 publications

(27 citation statements)

References 35 publications

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“…Dysregulation of miR-205 is one of the epigenetics events involved in the progression of melanoma. miR-205 regulates multiple cellular functions, including proliferation, migration and invasion, and is commonly altered in cancer 10 . Advances in the identification of the target genes for miR-205 may significantly contribute to delineating and understanding the molecular pathways involved in the metastatic dissemination of malignant melanoma when miR-205 expression is downregulated.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic identification of miR-205 target genes potentially involved in metastasis and survival of cutaneous malignant melanoma

Sánchez‐Sendra

Serna

Navarro

et al. 2020

Sci Rep

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Cutaneous melanoma is an aggressive neoplasm and is responsible for the majority of skin cancer deaths. Several miRNAs are involved in melanoma tumor progression. One of them is miR-205, the loss of which contributes to the development of melanoma metastasis. We evaluated whole-genome mRNA expression profiling associated with different miR-205 expression levels in melanoma cells. Differential expression analysis identified 243 differentially expressed transcripts including inositol polyphosphate 5′-phosphatase-like protein-1 (INPPL1) and BTB/POZ Domain-Containing Protein 3 (BTBD3). INPPL1 and BTBD3 were downregulated when melanoma cells expressed miR-205, indicating that these genes are potential miR-205 targets. Additionally, the target prediction algorithm TargetScan revealed that INPPL1 and BTBD3 genes had predicted target sites of miR-205 in their 3′UTRs and functional analysis demonstrated that these genes were directly linked to miR-205. Interestingly, our clinical data showed that INPPL1 was significantly associated with lymph node metastasis-free survival (LNMFS), distant metastasis-free survival (DMFS) and melanoma specific survival (MSS). This study supports INPPL1 as a miR-205 target gene and, therefore, that the involvement of miR-205 in the metastatic dissemination of malignant melanoma is, at least in part, via INPPL1. Most skin cancer related deaths are caused by melanoma, a malignant neoplasm with great potential to metastasize that is increasing in incidence and mortality 1. Epigenetic regulation of gene expression has, as in other cancer types, a significant role in melanoma 2. These epigenetic events include microRNAs (miRNAs). miRNAs are short noncoding RNAs with gene regulatory functions. miRNAs modulate gene expression by post-transcriptional repression, as they can direct mRNA cleavage or direct translational repression of their target protein-coding genes 3,4. Specific miRNAs have been implicated in many biological and pathological processes. miRNA impact on cancer depends on the functional nature of the targeted genes. In this way, miRNAs can produce both oncogenic or tumor suppressive effects by suppressing tumor suppressive mRNAs or oncogenic mRNAs, respectively. With regard to miR-205 target genes in melanoma, Dar et al. reported that miR-205 is potentially involved in melanomagenesis through suppression of cell proliferation and induction of senescence via regulation of E2F1 oncoprotein 5. It has also been suggested that miR-205 controls melanoma cell migration and invasion and that it inhibits the ZEB2 transcription factor 6. In particular, some miRNAs are known to be involved in the development and progression of melanoma 7-9. In this regard, we have recently demonstrated that the intratumoral downregulation of tumor suppressive miR-205 in primary melanomas contributes to the development of distant metastasis, and therefore to a shorter survival. Consequently, miR-205 intratumoral expression levels are useful to predict melanoma metastatic dissemination and patient survival 10. Our ...

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Section: Discussionmentioning

confidence: 99%

Transcriptomic identification of miR-205 target genes potentially involved in metastasis and survival of cutaneous malignant melanoma

Sánchez‐Sendra

Serna

Navarro

et al. 2020

Sci Rep

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“…Especially for primary melanomas, this approach showed the downregulation of intratumor expression for several miRNA species, such as miR-125b, miR-182, miR-200c, and miR-205, which could promote tumor dissemination. The TaqMan method could label miRNA-125b, miRNA-200c, and miRNA-205 as useful prognostic biomarkers correlated with shorter survival and, thus, able to select high-risk patients (Sanchez-Sendra et al, 2018). A notable version of the TaqMan method for miRNA detection is SplintR-qPCR, the major difference between the SplintR-qPCR and TaqMan being related to the tactics used for cDNA synthesis.…”

Section: Quantitative Pcr Methodsmentioning

confidence: 99%

“…The authors highlight that the downregulation of miR-205 alters the melanoma cells' interaction with the extracellular matrix favoring metastasis. The panel of miR-125b, miR-200c, and miR-205 can be developed in a prognostic biomarker panel and for selecting high-risk-recurrence patients (Sanchez-Sendra et al, 2018).…”

Section: Tumor Tissue Mirna Expression In Cutaneous Melanomamentioning

confidence: 99%

miRNAs in the Diagnosis and Prognosis of Skin Cancer

Neagu

Constantin

Creţoiu

et al. 2020

Front. Cell Dev. Biol.

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Skin cancer is, at present, the most common type of malignancy in the Caucasian population. Its incidence has increased rapidly in the last decade for both melanoma and non-melanoma skin cancer. Differential expression profiles of microRNAs (miRNAs) have been reported for a variety of different cancers, including skin cancers. Since miRNAs' discovery as regulators of gene expression, their importance grew in the field of oncology. miRNAs can post-transcriptionally regulate gene expression, tumor initiation, development progression, and aggressiveness. Nowadays, these short regulatory RNAs are perceived as one of the epigenetic markers for the identification of new diagnostic and/or prognostic molecular markers. Moreover, as miRNAs can drive tumorigenesis, they might eventually represent new therapy targets. Some miRNAs are pleiotropic, such as miR-214, which was found deregulated in several other tumors besides skin cancers. Some others are specific for one or more skin cancer types, like miR-21 and miR-221 for cutaneous melanoma and cutaneous squamous carcinoma or miR-155 for melanoma and cutaneous lymphoma. The goal of this review was to summarize some of the main miRNA detection technologies that are used to evaluate miRNAs in tissues and body fluids. Furthermore, their quantification limits, conformity, and robustness are discussed. Aberrant miRNA expression is analyzed for cutaneous melanoma, cutaneous squamous cell carcinoma (CSCC), skin lymphomas, cutaneous lymphoma, and Merkel cell carcinoma (MCC). In this type of disease, miRNAs are described as potential biomarkers to diagnose early lesion and/or early metastatic disease. In the future, whether in tissue or circulating in body fluids, miRNAs will gain their place in skin cancer diagnosis, prognosis, and future therapeutic targets.

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“…Intratumoral miR-NAs are involved in melanoma tumour progression and are able to predict melanoma survival. 2 Recently, circulating cell-free miRNAs (cfmiRNAs) have shown to have utility as non-invasive biomarkers in cancer, including melanoma. [3][4][5] Based on the study of cfmiRNAs, our aim was to improve the selection of high-risk melanoma patients at the time of diagnosis, in order to assess the convenience of monitoring disease progression by imaging surveillance and sentinel lymph node (SLN) analysis.…”

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confidence: 99%

“…The main plant photosensitizers in chronic actinic dermatitis are frullania, sesquiterpenic lactones and oak moss absolute. 2…”

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confidence: 99%

Non‐contact remote digital dermoscopy – new perspectives on differential diagnosis of inflammatory skin diseases

Heratizadeh

Fricke

Meinhardt‐Wollweber

et al. 2019

Acad Dermatol Venereol

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Downregulation of intratumoral expression of miR-205, miR-200c and miR-125b in primary human cutaneous melanomas predicts shorter survival

Cited by 27 publications

References 35 publications

Transcriptomic identification of miR-205 target genes potentially involved in metastasis and survival of cutaneous malignant melanoma

Transcriptomic identification of miR-205 target genes potentially involved in metastasis and survival of cutaneous malignant melanoma

miRNAs in the Diagnosis and Prognosis of Skin Cancer

Non‐contact remote digital dermoscopy – new perspectives on differential diagnosis of inflammatory skin diseases

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