Downregulation of <i>dystrophin</i> expression in pupae of the whitefly <i>Bemisia tabaci</i> inhibits the emergence of adults

Bar, Liliana K.; Czosnek, Henryk; Sobol, Iris; Ghanim, Murad; Shalev, A. Hariton

doi:10.1111/imb.12579

Cited by 7 publications

(5 citation statements)

References 56 publications

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“…With the advancements in next-generation sequencing and transcriptomics technology, several genes have been identified as potential targets to control B. tabaci and its viruses [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 56 , 57 , 58 ]. However, most studies are limited to the oral feeding of the dsRNA to B. tabaci through artificial diet or genetic modification of the host plants [ 40 , 43 , 59 , 60 ]. None of these RNAi molecules have been tested for their efficacy or found effective under field conditions, limiting their large-scale adoption.…”

Section: Discussionmentioning

confidence: 99%

“…Although several potential RNAi targets were identified for insects, including B. tabaci [ 40 , 41 , 42 , 43 ], most of those studies were conducted under controlled experimental conditions, and their efficacy under field conditions is limited. Rapid degradation of dsRNA in the extracellular environment and lack of reliable dsRNA delivery technique limits its applicability under field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Chakraborty

Ghosh

2022

Cells

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Chilli leaf curl virus (ChiLCV; genus: Begomovirus), transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in a persistent-circulative manner, is a major constraint in chilli production. The present study demonstrates for the first time that a topical spray of naked double-stranded RNA (dsRNA) on chilli plants causes mortality and inability to acquire and transmit ChiLCV in B. tabaci. dsRNA targeting heat shock protein 70 (hsp70) and fasciclin 2 (fas2) of B. tabaci Asia II 1 was first assessed under controlled conditions through oral delivery. Hsp70 and fas2 dsRNA resulted in up to 82.22% and 72% mortality of B. tabaci and around 12.4- and 8.5-fold decreases in mRNA levels, respectively, 24 h post-ingestion. ChiLCV copies in hsp70 dsRNA-fed B. tabaci steadily decreased with an increase in dsRNA concentration and were undetectable at a higher concentration of dsRNA. However, ChiLCV copies significantly increased in fas2 dsRNA-fed B. tabaci. Transmission of ChiLCV by B. tabaci was completely inhibited post-24 h feeding on hsp70 dsRNA at 3 μg/mL. Naked hsp70 dsRNA was topically sprayed on ChiLCV-infected chilli plants like an insecticide. 67.77% mortality of B. tabaci, 4.6-fold downregulation of hsp70 mRNA, and 1.34 × 1015-fold decreased ChiLCV copies in B. tabaci were recorded when adults were exposed to the dsRNA-treated plants under semi-field conditions. Foliar application of naked dsRNA reduced the ChiLCV transmission by 75% without any visible symptoms in the inoculated plants. A total of 2 consecutive sprays of dsRNA provided significant protection to B. tabaci for up to 20 days under semi-field conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Chakraborty

Ghosh

2022

Cells

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“…The development of mature whiteflies was dramatically reduced (48.6%) in the pupae produced on Dys-dsRNA-treated plants. [275] Tomato dsRNA applied exogenously to plants leaves.…”

Section: Exogenous Application Of Modified Dsrna Via Nrai Methodsmentioning

confidence: 99%

“…Genetic engineering techniques including transgenesis and RNA interference (RNAi) can be effective in regulating whitefly infestations. Transgenic crops harboring/synthesizing pesticidal toxins or lectins are useful in controlling whiteflies [35,275].…”

Section: Biotechnological Strategies For Whitefly Controlmentioning

confidence: 99%

Whitefly (Bemisia tabaci) Management (WFM) Strategies for Sustainable Agriculture: A Review

et al. 2022

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The whitefly (Bemisia tabaci Gennadius) is a notorious devastating sap-sucking insect pest that causes substantial crop damage and yield losses due to direct feeding by both nymphs and adults and also through transmission of viruses and diseases. Although the foliar application of synthetic pesticides is crucial for efficient control of B. tabaci, it has adverse effects such as environmental pollution, resistance and resurgence of the pest, toxicity to pollinators, and crop yield penalty. Thus, a suitable, safe, and robust strategy for the control of whiteflies in the agricultural field is needed. The reports on whitefly-resistant transgenic plants are scanty, non-reproducible, and/or need secondary trials and clearance from the Genetic Engineering Appraisal Committee (GEAC), the Ministry of Environment and Forests (MoEF), and the Environmental Protection Agency (EPA). The present review encompasses explicit information compiled from 364 articles on the traditional, mechanical, biological, biotechnological, and chemical strategies for whitefly management (WFM), IPM strategy, and future prospects of WFM for food and agriculture security.

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“…There are several genetic engineering approaches to developing crop plants' resistance to insects [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Expression of an Insecticidal Protein under Phloem Specific Promoter in Cotton Plants to Control Whitefly

Hussain

Aslam

Ahmed

et al. 2022

Preprint

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Whitefly the Bemisia tabaci is a phloem sap-sucking hemipteran pest that causes enormous damage to many crop plants, particularly cotton. Bt endotoxins which successfully controlled the chewing pest proved to be mostly ineffective against the whiteflies and many other hemipteran pests. In the past few years, some insecticidal proteins showed significant toxicity against the whiteflies such as Hvt, lectin, and TMA12. In this study, transgenic cotton lines expressing plant-derived insecticidal protein TMA12 under constitutive promoter (2X35S derived from Cauliflower mosaic virus-CaMV) and a phloem-specific promoter (Nuclear shuttle protein-NSP derived from Banana bunchy top virus-BBTV) were developed to compare their efficacy against the whiteflies. Before developing cotton transgenic lines, tobacco transgenic plants were also developed to get preliminary results about the efficacy of the transgene. After molecular characterization, cotton transgenic lines were evaluated for resistance against whitefly in the contained environment. Whitefly survival and fecundity were calculated for each transgenic line. Bioassay data proved the effectiveness of insecticidal protein against whiteflies. These results also concluded that the NSP promoter is potentially an effective strategy against B. tabaci but still its efficiency is not equal to the 2X35S promoter. The use of a phloem-specific promoter has advantages over the constitutive promoter like tissue-specific expression saves the molecular resources of the plant cells and is less prone to biosafety concerns. In the future, more phloem-specific promoters can be explored for enhanced effectivity against B. tabaci.

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Downregulation of dystrophin expression in pupae of the whitefly Bemisia tabaci inhibits the emergence of adults

Cited by 7 publications

References 56 publications

Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by Bemisia tabaci Asia II 1

Whitefly (Bemisia tabaci) Management (WFM) Strategies for Sustainable Agriculture: A Review

Expression of an Insecticidal Protein under Phloem Specific Promoter in Cotton Plants to Control Whitefly

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