Downregulation of dihydrolipoyl dehydrogenase by UVA suppresses melanoma progression via triggering oxidative stress and altering energy metabolism

Yumnam, Silvia; Kang, Moon Seong; Oh, Seung Hyun; Kwon, Hak Cheol; Kim, Jin Chul; Jung, Eun Joo; Lee, Choong Hwan; Lee, Ai Young; Hwang, Jong Ik; Kim, Sun Yeou

doi:10.1016/j.freeradbiomed.2020.11.037

Cited by 39 publications

(32 citation statements)

References 56 publications

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“…Metabolic decompensation caused by E3 deficiency may cause neurological symptoms that are often fatal [ 26 ]. Reduced DLD can induce autophagic cell death by producing reactive oxygen species (ROS) [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways

et al. 2022

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“…The synthesis of α-ketoglutarate from succinate is catalyzed by SUCLA2 [ 80 , 81 ]. However, stimulation of DLD can reduce α-ketoglutarate but increase succinate production [ 82 ], revealing that DLD up-regulation induced by high salinity stress in branchial cells may increase downstream metabolites of TCA cycle and promote mitochondrial ATP production. BCKDHB participates in α-ketoglutarate production in the process of energy generation [ 83 , 84 ].…”

Section: Discussionmentioning

confidence: 99%

Osmoregulatory strategies of estuarine fish Scatophagus argus in response to environmental salinity changes

Liu

Zhang

et al. 2022

BMC Genomics

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Background Scatophagus argus, an estuarine inhabitant, can rapidly adapt to different salinity environments. However, the knowledge of the molecular mechanisms underlying its strong salinity tolerance remains unclear. The gill, as the main osmoregulatory organ, plays a vital role in the salinity adaptation of the fish, and thus relative studies are constructive to reveal unique osmoregulatory mechanisms in S. argus. Results In the present study, iTRAQ coupled with nanoLC-MS/MS techniques were employed to explore branchial osmoregulatory mechanisms in S. argus acclimated to different salinities. Among 1,604 identified proteins, 796 differentially expressed proteins (DEPs) were detected. To further assess osmoregulatory strategies in the gills under different salinities, DEPs related to osmoregulatory (22), non-directional (18), hypo- (52), and hypersaline (40) stress responses were selected. Functional annotation analysis of these selected DEPs indicated that the cellular ion regulation (e.g. Na+-K+-ATPase [NKA] and Na+-K+-2Cl− cotransporter 1 [NKCC1]) and ATP synthesis were deeply involved in the osmoregulatory process. As an osmoregulatory protein, NKCC1 expression was inhibited under hyposaline stress but showed the opposite trend in hypersaline conditions. The expression levels of NKA α1 and β1 were only increased under hypersaline challenge. However, hyposaline treatments could enhance branchial NKA activity, which was inhibited under hypersaline environments, and correspondingly, reduced ATP content was observed in gill tissues exposed to hyposaline conditions, while its contents were increased in hypersaline groups. In vitro experiments indicated that Na+, K+, and Cl− ions were pumped out of branchial cells under hypoosmotic stress, whereas they were absorbed into cells under hyperosmotic conditions. Based on our results, we speculated that NKCC1-mediated Na+ influx was inhibited, and proper Na+ efflux was maintained by improving NKA activity under hyposaline stress, promoting the rapid adaptation of branchial cells to the hyposaline condition. Meanwhile, branchial cells prevented excessive loss of ions by increasing NKA internalization and reducing ATP synthesis. In contrast, excess ions in cells exposed to the hyperosmotic medium were excreted with sufficient energy supply, and reduced NKA activity and enhanced NKCC1-mediated Na+ influx were considered a compensatory regulation. Conclusions S. argus exhibited divergent osmoregulatory strategies in the gills when encountering hypoosmotic and hyperosmotic stresses, facilitating effective adaptabilities to a wide range of environmental salinity fluctuation.

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“…The similar results were also found in the next study that the mutated P icd with different activity were introduced in the strain Leu-4 (Table 2 and Figure 5a). One of the reasons is that more NADH was regenerated during increase of the activity of Icd Sm (Table 2 and Figure 5b), and thus to meet the demand of cell growth for energy (Yumnam et al, 2021). It should be noted that the strains with high Icd Sm activity showed the low yield and productivity of L-leucine because more carbon source was used for cell growth.…”

Section: Discussionmentioning

confidence: 99%

An NADPH-auxotrophic Corynebacterium glutamicum recombinant strain and used it to construct L-leucine high-yielding strain

Chen

Liu

Zhang

et al. 2022

Preprint

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The NADPH-regeneration enzymes in Corynebacterium glutamicum were inactivated to construct an NADPH-auxotrophic C. glutamicum strain by gene knockout and gene replacement. The resultant NADPH-auxotrophic C. glutamicum XL-1 ΔZMICg::ISm (i.e., strain Leu-1) grew well in the basic medium only with gluconate as carbon source. Replacement of the native glyceraldehyde 3-phosphate dehydrogenase (NAD-GapDHCg) by NADP-GapDHCa from Clostridium acetobutylicum is an effective strategy for producing L-leucine in NADPH-prototrophic strain XL-1 and NADPH-auxotrophic strain Leu-1, whereas the L-leucine yield did not differ significantly between these strains (14.1±1.8 g/L v.s. 16.2±1.1 g/L). Enhancing the carbon flux in biosynthetic pathway by recombinant expression plasmid pEC-ABNCE promoted L-leucine production, but the shortage NADPH supply limited the L-leucine yield. The mutated promoters of zwf and icdCg were introduced into C. glutamicum with NADP-GapDHCa and pEC-ABNCE increased L-leucine yield (32.8±1.7 g/L) and improved cell growth (OD562=41.3±4.2) because the resultant strain C. glutamicum XL-1 ΔMICg::ISm GCg::GCa Pzwf-D1 Picd-D2/pEC-ABNCE (i.e., strain Leu-9) exhibited the proper intracellular NADPH and NADH level. This is the first report of constructing an L-leucine high-yielding strain that reasonably supplies NADPH by optimizing the biosynthetic pathway of NADPH from an NADPH-auxotrophic strain.

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Downregulation of dihydrolipoyl dehydrogenase by UVA suppresses melanoma progression via triggering oxidative stress and altering energy metabolism

Cited by 39 publications

References 56 publications

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways

Osmoregulatory strategies of estuarine fish Scatophagus argus in response to environmental salinity changes

An NADPH-auxotrophic Corynebacterium glutamicum recombinant strain and used it to construct L-leucine high-yielding strain

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