Downregulation of circ_0037655 impedes glioma formation and metastasis via the regulation of miR-1229-3p/ITGB8 axis

Abstract

Background Glioma is the most frequent, highly aggressive primary intracranial malignant tumor. Circular RNA (circRNA) circ_0037655 has been reported to be a vital regulator in glioma. The different functional mechanism behind circ_0037655 was investigated in the current study. Methods The expression of circ_0037655, microRNA-1229-3p (miR-1229-3p) and integrin beta-8 (ITGB8) was detected via the quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Cellular research was performed via colony formation assay for cell proliferation, flow cytometry for cell cycle and cell apoptosis, scratch assay for cell migration, as well as transwell assay for cell migration and invasion. Western blot was used for detection of ITGB8 protein and epithelial–mesenchymal transition (EMT) process. Dual-luciferase reporter assay was implemented for the binding analysis of potential targets. In vivo assay was administered via xenograft in mice. Results Upregulation of circ_0037655 was affirmed in glioma samples and cells. Tumor formation and metastasis of glioma were inhibited after circ_0037655 was downregulated. miR-1229-3p acted as a target of circ_0037655, and its upregulation was responsible for the function of si-circ_0037655 in glioma cells. miR-1229-3p functioned as a tumor inhibitor in glioma progression by targeting ITGB8. circ_0037655 modulated the ITGB8 expression by targeting miR-1229-3p. In vivo knockdown of circ_0037655 also suppressed glioma tumorigenesis by acting on the miR-1229-3p/ITGB8 axis. Conclusion This study showed that downregulation of the expression of circ_0037655 could inhibit glioma progression by acting on the miR-1229-3p/ITGB8 axis. The specific circ_0037655/miR-1229-3p/ITGB8 axis was disclosed in glioma research.

Exosomal circDNER enhances paclitaxel resistance and tumorigenicity of lung cancer via targeting miR‐139‐5p/ITGB8

“…Integrin beta‐8 (ITGB8) has been shown to be essential for tumor cell survival and serves as a target gene of miR‐139‐5p according to our data. As a master regulator of angiogenesis, ITGB8 has previously been reported to correlate with poor prognosis in various malignancies 20 . In our study, we implicated for the first time that circ DNER promoted lung cancer proliferation, invasion and metastasis by modulating ITGB8 expression via sponging miR‐139‐5p, implying a novel regulatory axis formed by circ DNER /miR‐139‐5p/ITGB8 in lung cancer.…”

Differences in plasma miRNA levels in inferior petrosal sinus samples of patients with ACTH-dependent Cushing’s syndrome

“…В исследовании 2020 г. отмечено, что высокий уровень экспрессии miR-1229-3p в клетках аденокарциномы желудка и плазме крови пациентов ассоциирован с резистентностью к химиотерапии, в первую очередь к 5-фторурацилу, и потенциально может использоваться при выборе дальнейшей тактики ведения пациентов [48]. В другом исследовании было выявлено, что miR-1229-3p участвует в оси взаимодействия с интегрином-бета 8 (ITGB8), и при ее подавлении кольцевой РНК circ_0037655 наблюдается рост клеток глиомы и увеличивается риск метастазирования [49]. Повышенный уровень экспрессии miR-639 ассоциирован с более тяжелой стадией назофарингеальной карциномы и непосредственно влияет на пролиферацию и миграцию клеток [50].…”