Downregulation of cell surface CA125/MUC16 induces epithelial-to-mesenchymal transition and restores EGFR signalling in NIH:OVCAR3 ovarian carcinoma cells

Comamala, Marina; Pinard, Maxime; Thériault, Catherine; Matte, Isabelle; Albert, Anthony P.; Boivin, Micheline; Beaudin, Julie; Piché, Alain; Rancourt, Claudine

doi:10.1038/bjc.2011.34

Cited by 102 publications

(77 citation statements)

References 58 publications

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“…Also the increased cyclin A2 expression in migratory cells is an indication against EMT [58]. The only hint for maybe a partial EMT might the down-regulation of Muc16 in migratory cells [59]. Generally, the lack of detectable EMT in migratory RGM-1 cells is comparable with the situation in another non-transformed cell line, i.e.…”

Section: Discussionsupporting

confidence: 54%

TFF1 is Differentially Expressed in Stationary and Migratory Rat Gastric Epithelial Cells (RGM-1) afterin VitroWounding: Influence of TFF1 RNA Interference on Cell Migration

Kalbacher²,

Hoffmann

2013

Cell Physiol Biochem

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Background/Aims: The trefoil factor family (TFF) peptide TFF1 is typically secreted by gastric surface mucous cells. These cells are also the major players in gastric restitution, i.e., repair of the stomach mucosa by cell migration after injury. Methods: An established in vitro model of gastric restitution, i.e., migration of the non-transformed rat gastric cell line RGM-1 after scratch wounding, was investigated by expression profiling of selected genes from separated stationary and migratory cells. Also semi-quantitative immunocytochemistry was performed. Furthermore, RGM-1 cells were transfected with stealth RNAi™ duplexes targeting Tff1 and relative cell migration rates were analyzed. Results: Surprisingly, Tff1 expression was up-regulated in migratory cells. No unequivocal signs of the epithelial-mesenchymal transition were detectable in migratory cells. Transfection of RGM-1 cells with Tff1-siRNAi duplexes negatively influenced migration of these cells. Conclusion: This clearly points to a function of Tff1 as a motogen. A possible up-regulation of TFF1 synthesis in migratory surface mucous cells also in vivo would be an ideal mechanism to specifically enhance gastric restitution only where topologically needed and to minimize eventual negative side effects of TFF1 such as cell scattering and invasiveness.

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Section: Discussionsupporting

confidence: 54%

TFF1 is Differentially Expressed in Stationary and Migratory Rat Gastric Epithelial Cells (RGM-1) afterin VitroWounding: Influence of TFF1 RNA Interference on Cell Migration

Kalbacher²,

Hoffmann

2013

Cell Physiol Biochem

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“…Both MUC1 and EGFR are expressed by trophoblast and could conceivably interact at these surfaces; however, MUC1:EGFR interactions have not be demonstrated in trophoblast either. TMs also are implicated in promoting epithelial-to-mesenchymal transition (Comamala et al 2011 ;Ponnusamy et al 2013 ), although the relevance of this response to implantation is not clear.…”

Section: Tm Binding Signaling Proteinsmentioning

confidence: 98%

Transmembrane Mucin Expression and Function in Embryo Implantation and Placentation

Constantinou

Morgado

Carson

2015

Advances in Anatomy, Embryology and Cell Biology

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Transmembrane mucins (TMs) are extremely large, complex glycoproteins that line the apical surfaces of simple epithelia including those of the female reproductive tract. TMs provide a physical barrier consistent with their role as part of the innate immune system. This barrier function must be overcome in the context of embryo implantation to permit blastocyst attachment. Three major TMs have been identified in uterine epithelia of multiple species: MUC1, MUC4, and MUC16. MUC1 has been found in all species studied to date, whereas expression of MUC4 and MUC16 have been less well studied and may be species specific. The strategies for removing mucins to permit embryo attachment also vary in a species-specific way and include both hormonal suppression of TM gene expression and membrane clearance via cell surface proteases. Studies emerging from the cancer literature indicate that TMs can modulate a surprisingly wide variety of signal transduction processes. Furthermore, various cell surface proteins have been identified that bind either the oligosaccharide or protein motifs of TMs suggesting that these molecules may support cell attachment in some contexts, including trophoblast interactions with cells of the immune system. The intimate association of TMs at sites of embryo-maternal interaction and the varied functions these complex molecules can play make them key players in embryo implantation and placentation processes.

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“…CA125 is a repeating peptide epitope of the mucin MUC16 [17,18], which promotes cancer cell proliferation and inhibits anti-cancer immune responses [19][20][21][22]. CA125 has been extensively investigated as a biomarker in three separate clinical scenarios as a screening test for the early detection of cancer, to distinguish between benign and malignant disease and to monitor response to therapy.…”

Section: Discussionmentioning

confidence: 99%

Utility Of Salivary CA-125 And LDH As Tumor Markers In Oral Malignancy

Yadav

Ja²,

Ar³

et al. 2018

IJCBR

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Background:The mortality and morbidity associated with oral Squamous cell carcinoma (OSCC) can be greatly reduced if tumor markers which can detect OSCC at an early stage are available. This study attempts to use saliva as the diagnostic medium to determine the correlation of salivary CA 125 and LDH in tobacco users with and without potentially malignant disorders. Changes in CA 125 and LDH level can be used as a marker in patients with and without premalignant disease and can be used as a deterrent in continuation of the habit. Aims: To assess the role of CA 125 & LDH as a tumour marker in Oral cancer patients. Materials and methods: Cross sectional observational study .Time period: December 2015 to August 2017.Study area: OPD of tertiary care hospital in pune. Total participants: 150 persons. Under non-stimulatory conditions whole saliva sample was collected from each individual and was centrifuged. The resulting supernatant was used for estimation of CA 125 and LDH levels. Results: In present study, mean Salivary LDH level was found to be lowest among cases of healthy individuals while it was highest among individuals who were diagnosed and underwent treatment for malignancy. However there was insignificant difference seen between levels of diagnosed case of oral malignancy and the ones who underwent treatment for oral malignancy. Also mean Salivary CA-125 was lowest in healthy individuals and highest in individuals diagnosed with oral malignancy and the levels were seen to be reduced significantly in the one who underwent treatment for oral malignancies than those who didn't. Conclusion: Salivary CA 125 and LDH has a role in early detection of oral malignancies and can be used as a diagnostic marker. However LDH levels are persistently raised even after treatment for oral malignancy.

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Downregulation of cell surface CA125/MUC16 induces epithelial-to-mesenchymal transition and restores EGFR signalling in NIH:OVCAR3 ovarian carcinoma cells

Cited by 102 publications

References 58 publications

TFF1 is Differentially Expressed in Stationary and Migratory Rat Gastric Epithelial Cells (RGM-1) afterin VitroWounding: Influence of TFF1 RNA Interference on Cell Migration

TFF1 is Differentially Expressed in Stationary and Migratory Rat Gastric Epithelial Cells (RGM-1) afterin VitroWounding: Influence of TFF1 RNA Interference on Cell Migration

Transmembrane Mucin Expression and Function in Embryo Implantation and Placentation

Utility Of Salivary CA-125 And LDH As Tumor Markers In Oral Malignancy

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