Downregulation of c-Myc in pterygium and cultured pterygial cells

Cui, Dongmei; Pan, Zhujuan; Zhang, Shaochong; Zheng, Junnian; Huang, Qiang; Wu, Kaili

doi:10.1111/j.1442-9071.2011.02531.x

Cited by 8 publications

(3 citation statements)

References 33 publications

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“…Interferon-gamma response belongs to immune responses which have been associated with pterygium ( 58 ). MYC, as a proto-oncogene is also involved in the disease ( 59 ). The result of Wnt beta-catenin signaling is consistent with our previous GO analysis.…”

Section: Discussionmentioning

confidence: 99%

Expression profiling suggests the involvement of hormone-related, metabolic, and Wnt signaling pathways in pterygium progression

Tao

et al. 2022

Front. Endocrinol.

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BackgroundPterygium is an ocular surface disease that can cause visual impairment if it progressively invades the cornea. Although many pieces of research showed ultraviolet radiation is a trigger of pterygium pathological progress, the underlying mechanism in pterygium remains indistinct.MethodsIn this study, we used microarray to evaluate the changes of transcripts between primary pterygium and adjacent normal conjunctiva samples in China. Then, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses. Moreover, we constructed protein-protein interaction (PPI) and miRNA-mRNA regulatory networks to predict possible regulatory relationships. We next performed gene set enrichment analysis (GSEA) to explore the similarities and differences of transcripts between Asian studies from the Gene Expression Omnibus database. Furthermore, we took the intersection of differentially expressed genes (DEGs) with other data and identified hub genes of the development of pterygium. Finally, we utilized real-time quantitative PCR to verify the expression levels of candidate genes.ResultsA total of 49 DEGs were identified. The enrichment analyses of DEGs showed that pathways such as the Wnt-signaling pathway and metabolism-related pathways were upregulated, while pathways such as hormone-related and transcription factor-associated pathways were downregulated. The PPI and miRNA-mRNA regulatory networks provide ideas for future research directions. The GSEA of selecting Asian data revealed that epithelial-mesenchymal transition and myogenesis existed in the pathology of pterygium in the Asian group. Furthermore, five gene sets (interferon-gamma response, Wnt beta-catenin signaling, oxidative phosphorylation, DNA repair, and MYC targets v2) were found only in our Chinese datasets. After taking an intersection between selecting datasets, we identified two upregulated (SPP1 and MYH11) and five downregulated (ATF3, FOS, EGR1, FOSB, and NR4A2) hub genes. We finally chose night genes to verify their expression levels, including the other two genes (SFRP2 and SFRP4) involved in Wnt signaling; Their expression levels were significantly different between pterygium and conjunctiva.ConclusionsWe consider hormone-related, metabolic, and Wnt signaling pathways may be important in the pathology of pterygium development. Nine candidate genes we identified deserve further study and can be potential therapeutic targets.

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Section: Discussionmentioning

confidence: 99%

Expression profiling suggests the involvement of hormone-related, metabolic, and Wnt signaling pathways in pterygium progression

Tao

et al. 2022

Front. Endocrinol.

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“…The MAPK signal pathway, which is activated in nearly all cancers, has been shown to also be activated in pterygium tissue. [30][31][32] Loss of heterozygosity (LOH) and microsatellite instability (MI) are characteristics of transformed cells. [33][34][35] Detorakis et al 33 have found that LOH is common with pterygia most often at 9p (48%), followed by 17q (42%), but MI of DNA was uncommon.…”

Section: Discussionmentioning

confidence: 99%

K-ras oncogene mutation in pterygium

Öztürk

Yıldırım

Zamani

et al. 2016

Eye

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PurposePterygium is claimed to be a benign proliferation triggered by prolonged exposure to ultraviolet radiation. The frequency of K-ras oncogene mutation, which is among the initial mutations in tumorigenesis, is evaluated in this study.Patients and methodsIn this prospective randomized clinical, trial pterygium tissues and normal conjunctiva tissue specimens are obtained from the superotemporal quadrant of patients who underwent primary pterygium excision with autograft transplantation. DNA extraction from tissues was performed using the QIAamp DNA FFPE tissue kit. A PCR reaction was performed to amplify sequences containing codons 12, 13, and 61 of the K-ras gene in DNA. These PCR products then underwent the 'pyrosequencing' procedure for mutations at these codons.ResultsPterygium and normal conjunctival tissue samples of 25 patients (10 females, 15 males) were evaluated in the study. The mean age of the patients was 54.54±13.13 years. Genetic analysis revealed no K-ras mutations in normal conjunctival tissues, whereas pterygium tissues of the same cases demonstrated mutation at codon 12 in one case and mutations at codon 61 in seven cases, which was statistically significant (P<0.05). The point missense mutations at codon 61 were glutamine to arginine (Glu61Arg CAA>CGA) in four cases and glutamine to leucine (Glu61Leu CAA>CTA) in three cases.ConclusionThe significantly higher frequency of codon 61 mutation of the ras oncogene in primary and bilateral pterygium specimens compared with normal conjunctiva supports the tumoral origin of pterygium, and thus set the stage for research into a targeted therapy for pterygium with better outcomes than surgical excision.

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“…The ETC (Electronic Toll Collection) is designed to solve the traffic congestion problems and to improve the passage efficiency at the toll station. Some highways have set up the ETC lanes [1] in recent years, most of which are converted from the existing MTC lanes, and the traffic volume is significantly higher than ETC [2]. The toll station with ETC and MTC is called hybrid highway toll station.…”

Section: Introductionmentioning

confidence: 99%

Simulation and Experiment for Highway Toll Station

Kuang

Zhang

2012

AMM

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Toll station is the bottleneck of the highway. The simulation method is effective to analyze the traffic characteristics of highway toll station. The microscopic simulation model and the structure of toll station are built. The vehicle generation model, car-following model based on minimum safety distance, and the vehicle driving track selection model are analyzed. The simulation system is developed using VC++. The experiments with a dynamic adjustment method are studied using the simulation system, and the service level and vehicle passage rate are analyzed. The results show that the simulation system is suited to the real traffic characteristics and is an effective tool for highway toll station traffic analysis.

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Downregulation of c-Myc in pterygium and cultured pterygial cells

Abstract: c-Myc expression is reduced in pterygium compared with normal conjunctiva. Transforming growth factor beta1 can elevate the downregulated c-Myc in cultured pterygial cells.

Cited by 8 publications

References 33 publications

Expression profiling suggests the involvement of hormone-related, metabolic, and Wnt signaling pathways in pterygium progression

Expression profiling suggests the involvement of hormone-related, metabolic, and Wnt signaling pathways in pterygium progression

K-ras oncogene mutation in pterygium

Simulation and Experiment for Highway Toll Station

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