Downregulation of Bcl-2 and activation of caspase-8 in the UVB-induced apoptosis of a cultured human melanoma cell line

Abstract

Our data showed that the regulation of the Bcl-2 family and caspase-8 may work together to activate a caspase-3 mediated apoptotic pathway following UVB irradiation of cultured human melanoma cells.

Juglans regia L. protects against UVB induced apoptosis in human epidermal keratinocytes

“…Bax is capable of promoting apoptosis in the mitochondria-dependent cell death pathway, in which Cytochrome c and dATP dependent formation of the Apaf-1/Caspase-9 complex initiate an apoptotic protease cascade resulting in the cleavage of pro-caspases and activates downstream effecter caspases by activating Caspase-8. This suggests that p53, Bax and caspases contribute to the induction of apoptosis in human epidermal Keratinocytes via DNA damage due to UVB-irradiation [45] , [46] . In the present study, we found the up-regulation of p53, Bax, Cytochrome c, Caspases-9 and Caspases-3 ( Fig.…”

Effect of new antioxidant cysteinyl‐flavanol conjugates on skin cancer cells

“…Its incidence is rising at a rate of about 5% per year [1]. Some studies have shown that UV light exerts its biological effects, at least in part, via the generation of reactive oxygen species (ROS) and free radicals, which play a major role in the induction of skin cancer [2][3][4][5]. Chemopreventive approaches utilizing non-toxic agents aimed at both minimizing ROS formation and inducing apoptosis in tumour cells seem attractive.…”

Late Type Apoptosis and Apoptosis Free Lethal Effect of Quercetin in Human Melanoma

“…12,16) The main difference in methods between the two experiments was that in our case we irradiated the cells on ice to reduce the effect of heat produced by the UVB lamps during the irradiation. Heat is a well-known inducer of apoptosis.…”

“…[6][7][8][9] Quercetin shares with UVB some effects on melanoma cells such as inhibiting cell proliferation, inducing apoptosis, and decreasing Bcl-2 expression. [10][11][12][13] In contrast to quercetin, UVB's effects on human melanoma cell-lines and their underlying mechanisms have been thoroughly investigated. [14][15][16] Therefore, it is plausible to make inferences about the underlying mechanisms of action of quercetin from the similar effects of quercetin and UVB on the same melanoma cell-lines subjected to the same experimental conditions.…”