Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis

Wang, Runze; Peng, Cuixiu; Song, Junying; Hua, Yanan; Wu, Qinglan; Lin, Dehui; Cao, Yi; Zhang, Jinyu; Zhang, Li; Wu, Langping; Hou, Lin

doi:10.3892/ijo.2022.5323

Cited by 7 publications

(6 citation statements)

References 65 publications

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“…Increased ribosome biogenesis is necessary to maintain the elevated proliferation rate of tumor cells [ 34 ]. Recent research has implicated RRS1 in the development of several malignancies and it is significantly upregulated in various tumors [ 35 , 36 , 37 , 38 ]. The results of our previous studies have demonstrated the function of RRS1 in the growth of BC cells.…”

Section: Discussionmentioning

confidence: 99%

“…The GV492-GFP and GV115-EGFP lentiviral expression system (Shanghai GeneChem Co., Ltd.) was utilized for the transfection of cells expressing shRNA targeting RRS1 (sh-RRS1), control shRNA (sh-CON), scrambled control (OE-CON), and overexpressed RRS1 (OE-RRS1). The cells were subjected to infection with the corresponding lentiviruses at a multiplicity of infection (MOI) of 20 [ 37 , 38 ]. When the cells reached 20% to 30% confluence, they were transfected with the virus and Hitrans A&P (Shanghai GeneChem Co., Ltd.).…”

Section: Methodsmentioning

confidence: 99%

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Regulator of Ribosome Synthesis 1 (RRS1) Stabilizes GRP78 and Promotes Breast Cancer Progression

Sun,

Song,

et al. 2024

Molecules

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Regulator of ribosome synthesis 1 (RRS1), a crucial regulatory factor in ribosome biogenesis, exerts a remarkable impact on the progression of breast cancer (BC). However, the exact mechanisms and pathways have not yet been fully elucidated. To investigate the impact of RRS1 on BC growth and metastasis, along with its underlying mechanisms. We discovered that RRS1 is overexpressed in BC tissues and cell lines. This study aims to regulate the level of RRS1 through lentiviral transfection technology to explore its potential function in BC cells. Knockdown of RRS1 resulted in the inhibition of cell proliferation, invasion, and migration, whereas overexpression had the opposite effects. We firstly identified the interaction between RRS1 and Glucose-Regulated Protein 78 (GRP78) using Co-immunoprecipitation (Co-IP) combined with mass spectrometry analysis, providing evidences of co-localization and positive regulation between RRS1 and GRP78. We observed that RRS1 inhibited the degradation of GRP78 through the ubiquitin–proteasome pathway, resulting in the stabilization of GRP78. In addition, our findings suggested that RRS1 promoted BC progression by activating the GRP78-mediated phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. In conclusion, this newly discovered RRS1/GRP78 signaling axis provides a molecular and theoretical basis for further exploring the mechanisms of breast cancer invasion and metastasis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Regulator of Ribosome Synthesis 1 (RRS1) Stabilizes GRP78 and Promotes Breast Cancer Progression

Sun,

Song,

et al. 2024

Molecules

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“…RRS1 can promote the development of HCC by enhancing ribosome biogenesis and attenuating RPL11-MDM2-P53 signaling ( 48 ). Furthermore, RRS1 may augment breast cancer cell invasion and metastasis via the RPL11-c-Myc-SNAIL axis ( 49 ). Also, the downregulation of lncRNA SET-binding factor 2-antisense RNA1 can upregulate miR-143 and inhibit RRS1 and ultimately restrict the progression of breast cancer ( 50 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated bioinformatics and machine-learning screening for immune-related genes in diagnosing non-alcoholic fatty liver disease with ischemic stroke and RRS1 pan-cancer analysis

Bao

Zhang

et al. 2023

Front. Immunol.

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BackgroundThe occurrence of ischemic stroke (IS) is associated with nonalcoholic fatty liver disease (NAFLD). The cancer burden of NAFLD complicated by IS also warrants attention. This study aimed to identify candidate immune biomarkers linked to NAFLD and IS and analyze their association with cancer.MethodsTwo of each of the NAFLD and IS datasets were downloaded, differentially expressed genes (DEGs) were identified, and module genes were screened via weighted gene coexpression network analysis (WGCNA). Subsequently, utilizing machine learning (least absolute shrinkage and selection operator regression, random forest and support vector machine-recursive feature elimination) and immune cell infiltration analysis, immune-related candidate biomarkers for NAFLD with IS were determined. Simultaneously, a nomogram was established, the diagnostic efficacy was assessed, and the role of candidate biomarkers in cancer was ascertained through pan-cancer analyses.ResultsIn this study, 117 and 98 DEGs were identified from the combined NAFLD and IS datasets, respectively, and 279 genes were obtained from the most significant modules of NAFLD. NAFLD module genes and IS DEGs were intersected to obtain nine genes, which were enriched in the inflammatory response and immune regulation. After overlapping the results of the three machine learning algorithms, six candidate genes were obtained, based on which a nomogram was constructed. The calibration curve demonstrated good accuracy, and the candidate genes had high diagnostic values. The genes were found to be related to the immune dysregulation of stroke, and RRS1 was strongly associated with the prognosis, immune cell infiltration, microsatellite instability (MSI), and tumor mutation burden (TMB).ConclusionSix common candidate immune-related genes (PTGS2, FCGR1A, MMP9, VNN3, S100A12, and RRS1) of NAFLD and IS were identified, and a nomogram for diagnosing NAFLD with IS was established. RRS1 may serve as a candidate gene for predicting the prognosis of patients with cancer who have NAFLD complicated by IS, which could aid in their diagnosis and treatment.

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“…In this way, RPL11 reduces H4 acetylation at MYC target promoters inhibiting MYC-mediated transactivation. As RPL11 also affects MYC expression itself and vice versa, they function as a negative feedback loop ( Figure 1 C) [ 24 , 25 , 26 ]. In addition to ribosome biogenesis, MYC can modulate the isoform composition of RNA polymerase III, favoring the expression of the alpha isoform (POLR3G), which has a higher transcriptional potential.…”

Section: Overview Of Myc Regulation In the Physiological Statementioning

confidence: 99%

Rewired Metabolism Caused by the Oncogenic Deregulation of MYC as an Attractive Therapeutic Target in Cancers

Vízkeleti

Spisák

2023

Cells

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MYC is one of the most deregulated oncogenes on multiple levels in cancer. As a node transcription factor, MYC plays a diverse regulatory role in many cellular processes, including cell cycle and metabolism, both in physiological and pathological conditions. The relentless growth and proliferation of tumor cells lead to an insatiable demand for energy and nutrients, which requires the rewiring of cellular metabolism. As MYC can orchestrate all aspects of cellular metabolism, its altered regulation plays a central role in these processes, such as the Warburg effect, and is a well-established hallmark of cancer development. However, our current knowledge of MYC suggests that its spatial- and concentration-dependent contribution to tumorigenesis depends more on changes in the global or relative expression of target genes. As the direct targeting of MYC is proven to be challenging due to its relatively high toxicity, understanding its underlying regulatory mechanisms is essential for the development of tumor-selective targeted therapies. The aim of this review is to comprehensively summarize the diverse forms of MYC oncogenic deregulation, including DNA-, transcriptional- and post-translational level alterations, and their consequences for cellular metabolism. Furthermore, we also review the currently available and potentially attractive therapeutic options that exploit the vulnerability arising from the metabolic rearrangement of MYC-driven tumors.

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Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis

Cited by 7 publications

References 65 publications

Regulator of Ribosome Synthesis 1 (RRS1) Stabilizes GRP78 and Promotes Breast Cancer Progression

Regulator of Ribosome Synthesis 1 (RRS1) Stabilizes GRP78 and Promotes Breast Cancer Progression

Integrated bioinformatics and machine-learning screening for immune-related genes in diagnosing non-alcoholic fatty liver disease with ischemic stroke and RRS1 pan-cancer analysis

Rewired Metabolism Caused by the Oncogenic Deregulation of MYC as an Attractive Therapeutic Target in Cancers

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