Downregulated miR-18a and miR-92a synergistically suppress non-small cell lung cancer via targeting Sprouty 4

Abstract

As a novel noncoding RNA cluster, miR-17-92 cluster include six members: miR-17, miR-18a, miR-19a, miR-19b-1, miR-20a, and miR-92a. Dysregulation of miR-17-92 has been proved to be connected with the advancement of a series of human diseases, but the roles of miR-17-92 cluster in non-small cell lung cancer (NSCLC) have not been absolutely elaborated. Herein, we determined that miR-17-92 cluster were upregulated significantly in NSCLC tissues, and the cell proliferation, migration and cycle progression of NSCLC were also facilitated under the function of miR-17-92 cluster. Sprouty 4 ( SPRY4 ) was a direct target of miR-92a, and its overexpression restrained the exacerbation of NSCLC induced by miR-92a. Furthermore, the tumor xenograft assay showed that miR-92a facilitated tumor growth by inhibiting the expression of SPRY4 and mediating Epithelial-Mesenchymal Transition (EMT) in vivo . Finally, we looked into the synergistic effects of miR-92a and miR-18a on NSCLC, and found that antagomiR-18a treatment arrested the tumor growth rate of xenografted mice markedly.

WITHDRAWN: Mapping miRNA regulation of MGAT3 reveals upregulation as the dominant mode of action

“…31,32 Overexpression of our highest hit, miR-92a-1-5p is associated with progression and metastasis in lung, cervical and bone cancers. [33][34][35][36] This is in line with the known role of MGAT3 in cervical cancer.…”

Cell-free plasma miRNAs analysis for low invasive lung cancer diagnostics

“…Согласно данным литературы, внеклеточные микро-РНК крови представляют собой перспективные маркеры для диагностики онкологических заболеваний, в частности РЛ [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30], рака предстательной железы [11,22], остеосаркомы [12], рака яичников и др. Тем не менее диагностические тесты на основе анализа аберрантной экспрессии внеклеточных микроРНК не нашли широкого применения в клинических диагностических лабораториях.…”

“…В настоящем исследовании предпринята попытка разработки диагностической панели для выявления больных немелкоклеточным РЛ (НМРЛ) на основе нескольких внеклеточных микроРНК из плазмы крови и ВВ плазмы крови. Проанализированы концентрации 17 микроРНК, экспрессия которых, согласно полученным нами ранее результатам [11,12], а также анализу доступных данных литературы [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30], коррелирует с развитием РЛ в целом и НМРЛ в частности.…”

A label-free activatable biosensor for in situ detection of exosomal microRNAs based on DNA-AgNCs and hairpin type nucleic acid probes

“…5) for ovarian cancer, miR-1246 for breast cancer, 6 miR-205 (ref. 7) and miR-19a 8 for lung cancer, and miRNA-15a-5p for endometrial cancer. 9 Due to their low biodegradability and convenient sampling, exosomal miRNAs have received widespread attention as a non-invasive diagnostic biomarker for detecting cancer.…”