Down syndrome screening: Suitability of a WHO 5 standardized total hCG assay

Palomaki, Glenn E.; Lambert-Messerlian, Geralyn

doi:10.1016/j.clinbiochem.2014.01.013

Cited by 10 publications

(3 citation statements)

References 6 publications

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“…CEA is known as a marker of cancer of the digestive system and has been shown to be increased in levels in various cancers such as colon, stomach, and pancreas, etc. [ 40 ]. The anti-AFP-immobilized G-FET was immersed in hCG or CEA solutions for 2 h and then washed three times with 0.01 × PBS.…”

Section: Resultsmentioning

confidence: 99%

Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor

Kim

Park

et al. 2018

Sensors

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The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (ΔVDirac) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL−1 in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL−1, with a detection sensitivity of 5.68 mV. The sensitivity (ΔVDirac) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics.

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Section: Resultsmentioning

confidence: 99%

Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor

Kim

Park

et al. 2018

Sensors

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“…Our results showed that there was no statistically significant difference in the free β-hCG MoM value determined by the 6 laboratories. However, it is important to mention that laboratories implementing the new platform, which employs the new assay, will need to determine new reference values (medians), although this is not expected to have a marked effect on the maternal prenatal screening of T21 [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

External Quality Assessment of Maternal Serum Levels of Alpha-Fetoprotein, Free Beta-Human Chorionic Gonadotropin, and Unconjugated Estriol in Detecting Down Syndrome and Neural Tube Defects in the Second Trimester of 87 Maternal Serum Samples, Based on 105-139 Days

Chen

Shi³

et al. 2022

Med Sci Monit

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Background We aimed to insure the accuracy and reproducibility of alpha-fetoprotein (AFP), free beta-human chorionic gonadotropin (free β-hCG), and unconjugated estriol (uE3) concentrations for the screening for trisomy 21 (T21) and neural tube defects (NTD) in the second trimester. We conducted an external quality assessment of 6 laboratories, using maternal serum specimens. Material/Methods Serum specimens collected from 87 women of singleton pregnancies (4 with T21, 5 with NTD, and 78 with normal fetuses) were divided into 6 equivalent-volume fractions and transported to 6 laboratories (A, B, C, D, E, and F). All laboratories used the time-resolved fluorescence analyzer and supporting reagents to measure concentrations of AFP, free β-hCG, and uE3. The screening efficacies of T21 and NTD were compared with the certified or accredited status of the participants’ quality systems. Results Concentrations of AFP measured by laboratory F were low compared with those determined by the other 5 laboratories, and the differences were significant ( P <0.01). There was no statistically significant difference in the free β-hCG and uE3 concentrations measured by the 6 laboratories ( P >0.05). The correlation coefficients for the 3 multiples of the median values were all >0.900. The McNemar paired chi-squared test showed the differences in the positivity and detection rates were not statistically significant ( P =1.000). Conclusions AFP, freeβ-hCG, and uE3 values measured by the other 5 laboratories were comparable with those of laboratory A, with good linear correlation. When used in the maternal prenatal screening of T21 and NTD, the test results met the clinical requirements.

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“…Moreover, it is an important clinical parameter for the early diagnosis of ectopic pregnancy and in prenatal screening for Down's syndrome. 1 In addition to pregnancy, hCG is associated with male health problems such as testicular cancer. 2 Accordingly, the development of sensitive hCG immunoassays is desirable for both the investigation of pregnancy-related changes and the early diagnosis of cancer.…”

mentioning

confidence: 99%

Colorimetric detection of human chorionic gonadotropin using catalytic gold nanoparticles and a peptide aptamer

et al. 2014

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Down syndrome screening: Suitability of a WHO 5 standardized total hCG assay

Cited by 10 publications

References 6 publications

Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor

Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor

External Quality Assessment of Maternal Serum Levels of Alpha-Fetoprotein, Free Beta-Human Chorionic Gonadotropin, and Unconjugated Estriol in Detecting Down Syndrome and Neural Tube Defects in the Second Trimester of 87 Maternal Serum Samples, Based on 105-139 Days

Colorimetric detection of human chorionic gonadotropin using catalytic gold nanoparticles and a peptide aptamer

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