Down-Selection and Outdoor Evaluation of Novel, Halotolerant Algal Strains for Winter Cultivation

Dahlin, Lukas R.; Wychen, Stefanie Van; Gerken, Henri; McGowen, John; Pienkos, Philip T.; Posewitz, Matthew C.; Guarnieri, Michael T.

doi:10.3389/fpls.2018.01513

Cited by 22 publications

(20 citation statements)

References 47 publications

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Section: Resultsmentioning

confidence: 99%

“…We screened a >300-strain microalgal culture collection, in order to identify halotolerant strains 14,15 . Over 100 unique halotolerant isolates were down-selected and screened under simulated (diurnal light and temperature cycling) summer growth conditions using a custom built photobioreactor, described in Dahlin et al 15 . We identified one isolate that exhibited a noticeably faster growth rate relative to other isolates, including control strains Nannochloropsis oceanica (KA32) and Nannochloropsis salina (CCMP 1776), two top-candidate strains currently under evaluation for outdoor deployment (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

et al. 2019

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Microalgae are promising biocatalysts for applications in sustainable fuel, food, and chemical production. Here, we describe culture collection screening, down-selection, and development of a high-productivity, halophilic, thermotolerant microalga, Picochlorum renovo. This microalga displays a rapid growth rate and high diel biomass productivity (34 g m−2 day−1), with a composition well-suited for downstream processing. P. renovo exhibits broad salinity tolerance (growth at 107.5 g L−1 salinity) and thermotolerance (growth up to 40 °C), beneficial traits for outdoor cultivation. We report complete genome sequencing and analysis, and genetic tool development suitable for expression of transgenes inserted into the nuclear or chloroplast genomes. We further evaluate mechanisms of halotolerance via comparative transcriptomics, identifying novel genes differentially regulated in response to high salinity cultivation. These findings will enable basic science inquiries into control mechanisms governing Picochlorum biology and lay the foundation for development of a microalga with industrially relevant traits as a model photobiology platform.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

et al. 2019

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“…To realize the potential of microalgae, improvements are required in cost, productivity, and sustainability ( Dahlin et al, 2018 ). To scale up production at lower costs, open pond systems coupled with fresh water or wastewater are a viable alternative ( Bell et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…These systems are operated in semi-continuous or continuous mode to meet industrial production standards by maximizing both biomass and lipid productivities, while ensuring the industrial suitability of the microalga-derived fatty acids ( Song et al, 2013 ). However, although outdoor productivity is often less than the laboratory yield because of the extreme dynamics of environmental conditions, such as water temperature and light intensity, studies on identifying optimal strains have rarely investigated these criteria in semi-continuous or continuous cultures ( Dahlin et al, 2018 ). Recently, several studies questioned the sustainability of biofuel production from freshwater species because itis uneconomical ( Chisti, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation

et al. 2020

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Metagenome studies have provided us with insights into the complex interactions of microorganisms with their environments and hosts. Few studies have focused on microalgae-associated metagenomes, and no study has addressed aquatic microalgae and their bacterial communities in open pond raceways (OPRs). This study explored the possibility of using microalgal biomasses from OPRs for biodiesel and biofertilizer production. The fatty acid profiles of the biomasses and the physical and chemical properties of derived fuels were evaluated. In addition, the phenotype-based environmental adaptation ability of soybean plants was assessed. The growth rate, biomass, and lipid productivity of microalgae were also examined during mass cultivation from April to November 2017. Metagenomics analysis using MiSeq identified ∼127 eukaryotic phylotypes following mass cultivation with (OPR 1) or without (OPR 3) a semitransparent film. Of these, ∼80 phylotypes were found in both OPRs, while 23 and 24 phylotypes were identified in OPRs 1 and 3, respectively. The phylotypes belonged to various genera, such as Desmodesmus, Pseudopediastrum, Tetradesmus, and Chlorella, of which, the dominant microalgal species was Desmodesmus sp. On average, OPRs 1 and 3 produced ∼8.6 and 9.9 g m−2 d−1 (0.307 and 0.309 DW L−1) of total biomass, respectively, of which 14.0 and 13.3 wt% respectively, was lipid content. Fatty acid profiling revealed that total saturated fatty acids (mainly C16:0) of biodiesel obtained from the microalgal biomasses in OPRs 1 and 3 were 34.93% and 32.85%, respectively; total monounsaturated fatty acids (C16:1 and C18:1) were 32.40% and 31.64%, respectively; and polyunsaturated fatty acids (including C18:3) were 32.68% and 35.50%, respectively. Fuel properties determined by empirical equations were within the limits of biodiesel standards ASTM D6751 and EN 14214. Culture solutions with or without microalgal biomasses enhanced the environmental adaptation ability of soybean plants, increasing their seed production. Therefore, microalgal biomass produced through mass cultivation is excellent feedstock for producing high-quality biodiesel and biofertilizer.

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“…Manuscript to be reviewed are operated in semi-continuous or continuous mode to meet industrial production standards by maximizing both biomass and lipid productivities, while ensuring the industrial suitability of the microalga-derived fatty acids (Song et al, 2013). However, although outdoor productivity is often less than the laboratory yield because of the extreme dynamics of environmental conditions, such as water temperature and light intensity, studies on identifying optimal strains have rarely investigated these criteria in semi-continuous or continuous cultures (Dahlin et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation (v0.2)"

Li¹

2020

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Metagenome studies have provided us with insight into the complex interactions of microorganisms with their environments and hosts. Few studies have focused on microalgae-associated metagenomes, and no study has addressed aquatic microalgae and their bacterial communities in open pond raceways (OPRs). This study explored the possibility of using microalgal biomasses from OPRs for biodiesel and biofertilizer production. The fatty acid profiles of the biomasses and the physical and chemical properties of derived fuels were evaluated. In addition, the phenotype-based environmental adaptation ability of soybean plants was assessed. The growth rate, biomass, and lipid productivity of microalgae were also examined during mass cultivation from April to November 2017. Metagenomics analysis using MiSeq identified ~127 eukaryotic phylotypes following mass cultivation with (OPR 1) or without (OPR 3) a semitransparent film. Of these, ~80 phylotypes were found in both OPRs, while 23 and 24 phylotypes were identified in OPRs 1 and 3, respectively. The phylotypes belonged to various genera, such as Desmodesmus, Pseudopediastrum, Tetradesmus, and Chlorella, of which, the dominant microalgal species was Desmodesmus sp.. On average, OPRs 1 and 3 produced ~8.6 and 9.9 g•m-2 •d-1 (0.307 and 0.309 DW•L-1) of total biomass, respectively, of which 14.0 and 13.3 wt% respectively, was lipid content. Fatty acid profiling revealed that total saturated fatty acids (mainly C16:0) of biodiesel obtained from the microalgal biomasses in OPRs 1 and 3 were 34.93% and 32.85%, respectively; total monounsaturated fatty acids (C16:1 and C18:1) were 32.40% and 31.64%, respectively; and polyunsaturated fatty acids (including C18:3) were 32.68% and 35.50%, respectively. Fuel properties determined by empirical equations were within the limits of biodiesel standards ASTM D6751 and EN 14214. Culture solutions with or without microalgal biomasses enhanced

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Down-Selection and Outdoor Evaluation of Novel, Halotolerant Algal Strains for Winter Cultivation

Cited by 22 publications

References 47 publications

Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation

Peer Review #1 of "Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation (v0.2)"

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