Down's syndrome astrocytes have greater antioxidant capacity than euploid astrocytes

Sebastià, Jordi; Cristòfol, Rosa; Pertusa, Marı́a; Vı́lchez, David; Torán, Núria; Barambio, Santiago; Rodrı́guez-Farré, Eduard; Sanfeliu, Coral

doi:10.1111/j.1460-9568.2004.03686.x

Cited by 22 publications

(23 citation statements)

References 69 publications

(73 reference statements)

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“…The supernatants were collected and stored at -80 ºC until assayed. Enzyme activities and lipid peroxidation were determined as described previously (Sebastià et al, 2004). Lipid peroxidation was measured using a Lipid Peroxidation Assay Kit from Calbiochem (EMD Biosciences Inc., Darmstadt, Germany).…”

Section: Lipid Peroxidation Glutathione Peroxidase and Superoxide Dmentioning

confidence: 99%

Melatonin plus physical exercise are highly neuroprotective in the 3xTg-AD mouse

García-Mesa

Giménez-Llort

López

et al. 2012

Neurobiology of Aging

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Alzheimer's disease (AD) is a devastating age-related neurodegenerative disease with no specific treatment at present. Several healthy lifestyle options and over-the-counter drugs that it has been suggested delay the onset of the disease are in an experimental phase, but it is unclear whether they will have any therapeutic value against AD. We assayed physical exercise and melatonin in 3xTg-AD male mice aged from 6 to 12 months, therefore from moderate to advanced phases of AD pathology. Analysis of behavior and brain tissue at termination showed differential patterns of neuroprotection for the two treatments. Both treatments decreased soluble amyloid β oligomers, whereas only melatonin decreased hyperphosphorylated tau. Melatonin was effective against the immunosenescence that 3xTg-AD mice present. Voluntary physical exercise protected against behavioral and psychological symptoms of dementia such as anxiety, a lack of exploration and emotionality. Both treatments protected against cognitive impairment, brain oxidative stress and a decrease in mtDNA. Interestingly, only the combined treatment of physical exercise plus melatonin was effective against the decrease of mitochondrial complexes. Therefore, melatonin plus physical exercise may exert complementary, additive or even synergistic effects against a range of disturbances present in AD.

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Section: Lipid Peroxidation Glutathione Peroxidase and Superoxide Dmentioning

confidence: 99%

Melatonin plus physical exercise are highly neuroprotective in the 3xTg-AD mouse

García-Mesa

Giménez-Llort

López

et al. 2012

Neurobiology of Aging

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“…The OS which is present appears early in the fetus [51]. DS brain tissue is considered to be susceptible to oxidative injury, mainly because the increased SOD activity is not followed by an adaptive rise in enzymes that metabolize hydrogen peroxide [52]. The condition is characterized by increased lipid peroxidation, oxidative damage to DNA, and a rise in 8-OHdG, protein carbonyl and 3-nitrostyrene [53].…”

Section: Down's Syndrome (Ds)mentioning

confidence: 99%

Redox Processes in Neurodegenerative Disease Involving Reactive Oxygen Species

Kovacic¹,

Somanathan²

2012

Current Neuropharmacology

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Much attention has been devoted to neurodegenerative diseases involving redox processes. This review comprises an update involving redox processes reported in the considerable literature in recent years. The mechanism involves reactive oxygen species and oxidative stress, usually in the brain. There are many examples including Parkinson's, Huntington's, Alzheimer's, prions, Down's syndrome, ataxia, multiple sclerosis, Creutzfeldt-Jacob disease, amyotrophic lateral sclerosis, schizophrenia, and Tardive Dyskinesia. Evidence indicates a protective role for antioxidants, which may have clinical implications. A multifaceted approach to mode of action appears reasonable.

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“…Intracellular generation of hydroperoxides and superoxide anion radicals was determined using DCFH-DA and dihydroethidium, respectively, as previously described (Sebastià et al, 2004 …”

Section: Reactive Oxygen Species Generationmentioning

confidence: 99%

Dysfunction of astrocytes in senescence‐accelerated mice SAMP8 reduces their neuroprotective capacity

et al. 2008

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SummaryEarly onset increases in oxidative stress and tau pathology are present in the brain of senescence-accelerated mice prone (SAMP8). Astrocytes play an essential role, both in determining the brain's susceptibility to oxidative damage and in protecting neurons. In this study, we examine changes in tau phosphorylation, oxidative stress and glutamate uptake in primary cultures of cortical astrocytes from neonatal SAMP8 mice and senescenceaccelerated-resistant mice (SAMR1). We demonstrated an enhancement of abnormally phosphorylated tau in Ser 199 and Ser 396 in SAMP8 astrocytes compared with that of SAMR1 control mice. Gsk3β β β β and Cdk5 kinase activity, which regulate tau phosphorylation, was also increased in SAMP8 astrocytes. Inhibition of Gsk3β β β β by lithium or Cdk5 by roscovitine reduced tau phosphorylation at Ser 396 .Moreover, we detected an increase in radical superoxide generation, which may be responsible for the corresponding increase in lipoperoxidation and protein oxidation. We also observed a reduced mitochondrial membrane potential in SAMP8 mouse astrocytes. Glutamate uptake in astrocytes is a critical neuroprotective mechanism. SAMP8 astrocytes showed a decreased glutamate uptake compared with those of SAMR1 controls. Interestingly, survival of SAMP8 or SAMR1 neurons cocultured with SAMP8 astrocytes was significantly reduced. Our results indicate that alterations in astrocyte cultures from SAMP8 mice are similar to those detected in whole brains of SAMP8 mice at 1-5 months. Moreover, our findings suggest that this in vitro preparation is suitable for studying the molecular and cellular processes underlying early aging in this murine model. In addition, our study supports the contention that astrocytes play a key role in neurodegeneration during the aging process.

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Down's syndrome astrocytes have greater antioxidant capacity than euploid astrocytes

Cited by 22 publications

References 69 publications

Melatonin plus physical exercise are highly neuroprotective in the 3xTg-AD mouse

Melatonin plus physical exercise are highly neuroprotective in the 3xTg-AD mouse

Redox Processes in Neurodegenerative Disease Involving Reactive Oxygen Species

Dysfunction of astrocytes in senescence‐accelerated mice SAMP8 reduces their neuroprotective capacity

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