Down-regulation of surface monocyte lipopolysaccharide-receptor cd14 in patients on cardiopulmonary bypass undergoing aorta-coronary bypass operation

Fingerle‐Rowson, Günter; Auersa, J.; Kreuzer, E.; Labéta, Mario O.; Schmidta, B.; Samtleben, Werner; Ziegler‐Heitbrock, H. W. L.; Blumenstein, M

doi:10.1016/s0022-5223(98)70418-0

Cited by 25 publications

(19 citation statements)

References 30 publications

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“…Only a transient downregulation of CD14 surface expression was observed. Decrease in CD14 expression on blood monocytes were reported in septicemic patients (42)(43)(44) and has been associated with apoptosis of monocytes (45) that was also observed in our culture conditions. This suggested that interaction with CHIKV did not lead to obvious monocytes activation.…”

Section: Discussionsupporting

confidence: 84%

Active Infection of Human Blood Monocytes by Chikungunya Virus Triggers an Innate Immune Response

Her

Malleret

Chan

et al. 2010

The Journal of Immunology

250

254

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Chikungunya virus (CHIKV) is an alphavirus that causes chronic and incapacitating arthralgia in humans. To date, interactions between the immune system and the different stages of the virus life cycle remain poorly defined. We demonstrated for the first time that CHIKV Ags could be detected in vivo in the monocytes of acutely infected patients. Using in vitro experimental systems, whole blood and purified monocytes, we confirmed that monocytes could be infected and virus growth could be sustained. CHIKV interactions with monocytes, and with other blood leukocytes, induced a robust and rapid innate immune response with the production of specific chemokines and cytokines. In particular, high levels of IFN-α were produced rapidly after CHIKV incubation with monocytes. The identification of monocytes during the early phase of CHIKV infection in vivo is significant as infected monocyte/macrophage cells have been detected in the synovial tissues of chronically CHIKV-infected patients, and these cells may behave as the vehicles for virus dissemination. This may explain the persistence of joint symptoms despite the short duration of viremia. Our results provide a better understanding on the basic mechanisms of infection and early antiviral immune responses and will help in the development of future effective control strategies.

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Section: Discussionsupporting

confidence: 84%

Active Infection of Human Blood Monocytes by Chikungunya Virus Triggers an Innate Immune Response

Her

Malleret

Chan

et al. 2010

The Journal of Immunology

250

254

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“…A decrease in monocyte count after initiation of CPB and monocytosis the morning after the CABG have been reported. 33,35 We observed a similar variation in our flow cytometry analyses (data not shown). This must be taken into account, evaluating the significance of monocyte surface receptor regulation.…”

Section: Discussionsupporting

confidence: 80%

“…However, monocyte CD14 expression after CABG is shown to be reduced the first postoperative day. 33 This downregulation of monocyte surface antigens probably represents a deactivation of monocytic proinflammatory functions, 34 and persistence of the deactivation may be associated with increasing risk of microbial invasion and infectious complications. We observed an initial downregulation of monocyte CD14, TLR-2, and TLR-4 after the CABG operation.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Response After Open Heart Surgery

et al. 2002

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Background-Coronary artery bypass grafting with the use of cardiopulmonary bypass is known to mediate an inflammatory response. The stress-inducible heat-shock protein (HSP) 70 has been detected in myocardial cells after CABG, and toll-like receptors (TLRs) are suggested as putative signaling receptors for the HSPs, mediating synthesis of inflammatory cytokines. The main aims of our study were to explore the release of HSP70 and the regulation of monocyte TLR-2 and TLR-4 expression after CABG. Methods and Results-Twenty patients referred for elective CABG were included in this study. Using immunoassays, we detected HSP70 in plasma after CABG, with peak concentration immediately after surgery. Interleukin-6 in plasma reached peak concentration 5 hours after surgery. Monocyte CD14, TLR-2, and TLR-4 expression, as analyzed by flow cytometry, was initially downregulated. On day 1, CD14 expression normalized, whereas TLR-2 and TLR-4 expression was upregulated. TLR-4 was significantly upregulated even on postoperative day 2. Additional experiments revealed that peritoneal macrophages from control (C3H/HeN) mice responded to HSP70 with release of tumor necrosis factor, whereas macrophages from mutated TLR-4 (C3H/HeJ) mice were unresponsive. In vitro, human adherent monocytes responded to recombinant HSP70 with interleukin-6 and tumor necrosis factor release. CD14 and TLR-4 monoclonal antibodies inhibited the cytokine response. Conclusions-In this study, we observed an immediate release of HSP70 into the circulation and a modulation of monocyte TLR-2 and TLR-4 expression after CABG. TLR-4 and CD14 appear to be involved in an HSP70-mediated activation of innate immunity. (Circulation. 2002;105:685-690.)

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“…Downregulation of the MHCII receptor was demonstrated in both on-bypass and OPCAB groups as previously described [6], whereas LPS receptor (CD14) expression only declined following on-bypass surgery. This drop in receptor expression may further potentiate immunoinflammatory resolution following cardiac surgery by reducing antigen presentation and endotoxin-mediated cytokine release, respectively [19]. Similar to CD163, monocyte CD14 is shed into the plasma during CPB surgery and minimises endotoxic effects by complexing with LPS [19].…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery

Philippidis¹,

Athanasiou²,

Nadra³

et al. 2010

European Journal of Cardio-Thoracic Surgery

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Coronary artery bypass surgery induces monocyte-associated anti-inflammatory and immunoregulatory responses. There was no significant difference in haemolysis although the effect on monocyte CD163 and plasma sCD163 concentration was significantly different between the two groups. Compensation for varying pro-inflammatory and haemolytic insults may explain the differences observed. Therapeutic strategies for inducing such desirable circulating monocytes may potentially improve surgical outcome and patient recovery.

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Down-regulation of surface monocyte lipopolysaccharide-receptor cd14 in patients on cardiopulmonary bypass undergoing aorta-coronary bypass operation

Cited by 25 publications

References 30 publications

Active Infection of Human Blood Monocytes by Chikungunya Virus Triggers an Innate Immune Response

Active Infection of Human Blood Monocytes by Chikungunya Virus Triggers an Innate Immune Response

Inflammatory Response After Open Heart Surgery

Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery

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