Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma

Okamoto, Atsushi; Higo, Morihiro; Shiiba, Masashi; Nakashima, Daisuke; Koyama, Tomoyoshi; Miyamoto, Isao; Kasama, Hiroki; Kasamatsu, Atsushi; Ogawara, Katsunori; Yokoe, Hidetaka; Tanzawa, Hideki; Uzawa, Katsuhiro

doi:10.1371/journal.pone.0142252

Cited by 43 publications

(41 citation statements)

References 22 publications

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“…Given its critical role in mitosis, knockdown of NUSAP1 results in decreased proliferation, cell cycle arrest in G2/M, and induction of apoptosis [26, 56, 57], as we have observed here. Transient high-level overexpression of NUSAP1 can also be toxic to cells [26, 56], limiting previous efforts to study its potential role in carcinogenesis and progression.…”

Section: Discussionsupporting

confidence: 63%

NUSAP1 promotes invasion and metastasis of prostate cancer

et al. 2017

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We have previously identified nucleolar and spindle associated protein 1 (NUSAP1) as a prognostic biomarker in early stage prostate cancer. To better understand the role of NUSAP1 in prostate cancer progression, we tested the effects of increased and decreased NUSAP1 expression in cell lines, in vivo models, and patient samples. NUSAP1 promotes invasion, migration, and metastasis, possibly by modulating family with sequence similarity 101 member B (FAM101B), a transforming growth factor beta 1 (TGFβ1) signaling effector involved in the epithelial to mesenchymal transition. Our findings provide insights into the importance of NUSAP1 in prostate cancer progression and provide a rationale for further study of NUSAP1 function, regulation, and clinical utility.

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Section: Discussionsupporting

confidence: 63%

NUSAP1 promotes invasion and metastasis of prostate cancer

et al. 2017

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“…Similar biological functions of NUSAP1 were also observed in other malignancies, including breast cancer, hepatocellular carcinoma (HCC), glioblastoma multiforme (GBM), colorectal cancer, astrocytoma, glioma, renal cell carcinoma, and prostate cancer . And previous studies also reported downregulating NUSAP1 could enhance the susceptibility to epirubicin in invasive breast cancer and the anti‐tumour effect of paclitaxel in OSCC . In addition, high NUSAP1 expression predicted shorter survival in breast cancer, HCC, GBM, colon cancer, and glioma .…”

Section: Introductionsupporting

confidence: 56%

“…In cervical carcinoma, Li et al illuminated that NUSAP1 could promote cancer stem cell properties and epithelial‐mesenchyme transition (EMT) through activing Wnt/β‐catenin signalling, thereby accelerating cell migration and invasion. Okamoto et al revealed high NUSAP1 expression was related to more advanced T stage and inhibiting NUSAP1 resulted in the suppression of cell proliferation in Oral Squamous Cell Carcinoma (OSCC). Similar biological functions of NUSAP1 were also observed in other malignancies, including breast cancer, hepatocellular carcinoma (HCC), glioblastoma multiforme (GBM), colorectal cancer, astrocytoma, glioma, renal cell carcinoma, and prostate cancer .…”

Section: Introductionmentioning

confidence: 99%

Downregulation of NUSAP1 suppresses cell proliferation, migration, and invasion via inhibiting mTORC1 signalling pathway in gastric cancer

Lin

et al. 2019

Cell Biochemistry & Function

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Gastric cancer (GC) is one of the most common causes of cancer-related death worldwide, and outstanding biomarkers for therapeutic targets or predicting GC survival are still lacking. Increasing evidence indicated that nucleolar and spindle associated protein 1 (NUSAP1) involved in regulating the progression of various cancers; however, its specific role in GC remained unclear. In this study, we found that NUSAP1 was upregulated in the GC tissues and cell lines via analysing data from The Cancer Genome Atlas (TCGA), gene expression omnibus (GEO), qRT-PCR, and western blot assays. Patients with high NUSAP1 expression levels showed shorter freeprogression survival (FPS), larger tumour size, and higher lymphatic metastasis rate compared with those with low NUSAP1 expression. Further functional experiments revealed knockdown of NUSAP1 could inhibit the growth, migration, and invasion of GC cells in vitro and vivo. Additionally, silencing NUSAP1 induced G0/G1 phase arrest, apoptosis, and suppressed the epithelial-mesenchymal transition (EMT) process. Finally, we performed gene set enrichment analysis (GSEA) and observed NUSAP1 was positive with mTORC1 signalling pathway, which was verified by the subsequent immunoblotting. In conclusion, our findings suggested that NUSAP1 contributed to GC progression and may act as a potential therapeutic target for GC.Significance of the study: Our results firstly illuminated that NUSAP1 expression was significantly upregulated in GC tissues and predicted poor FPS. Silencing it could attenuate GC progression via inhibiting mTORC1 signalling pathway. Hence, NUSAP1 may act as a promising therapy target for GC.

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“…Nucleolar and spindle‐associated protein 1 has been identified to play an important role in carcinogenesis. A higher expression pattern of NUSAP1 has been found in many kinds of cancers and serves as a prognostic factor, including liver cancer, breast cancer, oral squamous carcinoma, prostate cancer, pancreatic cancer, glioblastoma, and melanoma . NUSAP1 was reported to be overexpressed in bladder cancer and overexpression significantly enhanced the stemness of bladder cancer cells .…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer

Chen

Zhang

Kadier

et al. 2020

Clinical Laboratory Analysis

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Background Nucleolar and spindle‐associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioinformatics methods, we found that NUSAP1 was a putative target of miR‐769‐5p. Here, we aimed to explore whether miR‐769‐5p is involved in bladder cancer progression via targeting NUSAP1. Methods MiR‐769‐5p expression patterns in bladder cancer tissues and cells were detected by RT‐PCR. Kaplan‐Meier was used to determine the clinical effects of miR‐769‐5p expression levels on the overall survival of bladder cancer patients. Bioinformatics methods were used to predict the binding sites between miR‐769‐5p and NUSAP1, which was verified by the luciferase gene reporter assay. CCK‐8, flow cytometry, wound healing and transwell chamber experiments were performed to test cell growth, apoptosis, migration and invasion capacities. Results miR‐769‐5p was lowly expressed in bladder cancer tissues and cells, which was closely associated with poor prognosis. Overexpression of miR‐769‐5p induced significant repressions in cell growth, migration, and invasion and caused an obvious increase in cell apoptosis, whereas these tendencies were reversed when NUSAP1 was upregulated. Conclusion This study demonstrates that miR‐769‐5p functions as a tumor suppressor in bladder cancer via targeting NUSAP1.

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Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma

Cited by 43 publications

References 22 publications

NUSAP1 promotes invasion and metastasis of prostate cancer

NUSAP1 promotes invasion and metastasis of prostate cancer

Downregulation of NUSAP1 suppresses cell proliferation, migration, and invasion via inhibiting mTORC1 signalling pathway in gastric cancer

MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer

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