Down Regulation of Murine MHC Class I Expression by Bovine Herpesvirus 1

Abstract

The objective of this study was to investigate the effect(s) of bovine herpesvirus 1 (BHV-1) infection on the expression of MHC class I molecules in murine fibroblasts. L-cells were infected with BHV-1 at a multiplicity of infection (m.o.i.) of 10 plaque forming units (PFU) per cell, and the expression of MHC class I molecules was analyzed by flow cytometry and immunoprecipitation. Temporal studies revealed a reduction in class I expression beginning at 8 h post infection (p.i.) which reached a maximum between 10 to 16 h p.i. The loss of class I expression was restored in the presence of phosphonoacetic acid (30 micrograms/ml), a late herpesviral protein synthesis inhibitor. However, addition of cycloheximide, a total protein synthesis inhibitor (100 micrograms/ml), did not result in any difference in class I expression between virus-infected and mock-infected cells. These results suggest that the reduced class I expression is a direct consequence of BHV-1 infection, and that the late viral gene product(s) may be involved in this process. Similar phenomena may occur in natural BHV-1 infection in cattle, and this may be one of the mechanisms of immune suppression by BHV-1.

Localization of Sequences in a Protein (ORF2) Encoded by the Latency-Related Gene of Bovine Herpesvirus 1 That Inhibits Apoptosis and Interferes with Notch1-Mediated trans -Activation of the bICP0 Promoter

“…Increased susceptibility to secondary infection correlates with depressed cell-mediated immunity after BHV-1 infection (6,(16)(17)(18). CD8 ϩ T cell recognition of infected cells is impaired by repressing the expression of major histocompatibility complex class I and the transporter associated with antigen presentation (20,24,57). CD4 ϩ T cell function is impaired during acute infection of calves, because BHV-1 infects CD4 ϩ T cells and induces apoptosis (73).…”

A Mutation in the Latency-Related Gene of Bovine Herpesvirus 1 Disrupts the Latency Reactivation Cycle in Calves

“…ϩ T-cell recognition of infected cells is impaired by repressing expression of major histocompatibility complex class I and of the transporter associated with antigen presentation (13,14,25). CD4…”

Infection of Cattle with a Bovine Herpesvirus 1 Strain That Contains a Mutation in the Latency-Related Gene Leads to Increased Apoptosis in Trigeminal Ganglia during the Transition from Acute Infection to Latency

“…ϩ -T-cell recognition of infected cells is impaired because the expression of major histocompatibility complex class I and antigen presentation are inhibited (21,24,41). CD4…”