Down-regulation of miR-19b-3p enhances IGF-1 expression to induce osteoblast differentiation and improve osteoporosis

Abstract

This research was conducted in order to investigate the role of miR-19b-3p in the development of osteoporosis (OP) in rats and the associated mechanisms. This study measured the expression levels of miR-19b-3p and IGF-1 in clinical OP patients and ovariectomy-induced OP rats by qRT-PCR. The osteoprotegerin levels in OP patients were measured by enzyme-linked immunosorbent assay (ELISA). The binding site of miR-19b-3p to IGF-1 was predicted by three prediction sites: Target Scan, miRDB and starbase. Experiments were conducted in vitro and in vivo using bone marrow mesenchymal stem cells (BMSCs) and OP rats, respectively, to verify the regulatory relationship between miR-19b-3p and IGF-1 and explore the role of miR-19b-3p in the development of OP. Results showed that the expression of miR-19b-3p was elevated in OP patients and rats, while IGF-1 expression was decreased (***p<0.001). The ELISA assay found that osteoprotegerin levels were inversely correlated with miR-19b-3p and positively correlated with IGF-1. The predictive analysis identified binding sites for miR-19b-3p to IGF-1. The potential regulatory relationship between miR-19b-3p and IGF-1 was validated by in vitro and in vivo experiments. Moreover, the important role of miR-19b-3p in the regulation of OP was further demonstrated. It was concluded the inhibition of miR-19b-3p has a suppressive effect on the development of OP and the function of miR-19b-3p in OP is likely to be achieved by regulating the expression of the IGF-1 gene.

Exploring the mechanism of Wuzi Yanzong Pill in treating menopausal osteoporosis based on bioinformatics and network pharmacology

“…-19b-3p was found to be signi cantly increased in postmenopausal women with osteoporosis and BMSCs induced with BMP-2. Increasing miR-19b-3p led to a signi cant increase in BMSCs cell growth, while inhibiting miR-19b-3p resulted in decreased cell proliferation(Yu et al, 2022;Xiaoling et al, 2020). These studies support and validate our ndings, demonstrating that the active components of WZYZP effectively treat perimenopausal osteoporosis by regulating the expression of hsa-miR-19b-3p and hsa-miR-1294.Quercetin and kaempferol are the most effective chemical constituents of Cuscuta chinensis.…”

The bioceramic sealer iRoot SP promotes osteogenic differentiation of human stem cells from apical papilla via miR‐141‐3p/SPAG9/MAPK signalling pathway

“…Many of these miRNAs are involved in osteogenic differentiation of cells. For example, miR‐19b‐3p inhibits IGF‐1 expression, thereby preventing the differentiation of BMSCs into osteoblasts, leading to the development of osteoporosis (Yu et al, 2022). MiR‐138‐5p contributes to bone loss in the elderly by downregulating MACF1 expression and inhibiting osteogenic differentiation (Chen et al, 2022).…”

Clinical efficacy of Gushukang Jiaonang in osteoporosis and its effects on bone metabolism in patients: A retrospective study

“…The process of bone resorption and bone reconstruction is influenced by various humoral, biochemical, mechanical and other environmental factors as well as hormones and various growth factors. The autocrine effects of local regulatory factors have direct or indirect physiopathological regulation of proliferation, differentiation, matrix synthesis and mineralization of bone spectrum cells [7]. Impairment of this regulatory mechanism leads to loss of balance in bone formation-resorption coupling, resulting in bone loss [8].…”