Down-regulation of MicroRNA-381 promotes cell proliferation and invasion in colon cancer through up-regulation of LRH-1

Liang, Yan; Zhao, Qun; Fan, Li; Zhang, Zhidong; Tan, Bibo; Liu, Yü; Li, Yong

doi:10.1016/j.biopha.2015.07.020

Cited by 44 publications

(45 citation statements)

References 20 publications

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“…miRNAs exert regulatory actions in a way of cutting, and promote the up-regulation or down-regulation of mRNA expression to mediate the regulation of protein-coding genes, playing important roles in the occurrence and development of diseases (20,21). It is discovered that down-regulation of miR-381 in colon cancer tissues leads to up-regulation of LRH-1 and induces proliferation and invasion of colon cancer cells (22). In addition, miR-381, which is present in the p53/PTTG1 negative feedback loop, can inhibit the growth of pituitary adenomas (23).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑381 protects myocardial cell function in children and mice with viral myocarditis via targeting cyclooxygenase‑2 expression

Zhang

Sun

et al. 2018

Exp Ther Med

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Abstract. The present study aimed to determine the expression of cyclooxygenase (COX)-2 and microRNA (miRNA/miR)-381 in the blood of children with viral myocarditis (VM), and investigate the association between COX-2 and miR-381 in the occurrence and development of the disease using a mouse model. A total of 26 children with VM (15 boys and 11 girls) were included in the present study. Peripheral blood was collected from all children. The mouse model of VM was constructed by coxsackievirus B3 (CVB3) infection. Peripheral blood and myocardial tissues were collected from all mice for analysis. Reverse transcription-quantitative polymerase chain reaction was used to determine the expression of COX-2 mRNA and miR-381 in serum and myocardial tissues. ELISA was used to measure the content of COX-2 protein in serum from humans and mice, and western blotting was employed to determine the expression of COX-2 protein in myocardial tissues from mice. Contents of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) were evaluated using an automatic biochemical analyzer. A dual luciferase assay was conducted to identify interactions between COX-2 mRNA and miR-381. Children with VM had increased COX-2 levels and decreased miR-381 expression in peripheral blood, compared with those who had recovered from VM. CVB3 infection resulted in damage in the myocardium of mice, and elevated CK-MB and LDH contents. VM model mice exhibited increased COX-2 levels and decreased miR-381 expression in peripheral blood and myocardial tissues compared with normal mice. miR-381 binds to the 3'-untranslated seed regions of both human and mouse COX-2 mRNA to regulate their expression. The present study demonstrated that children with VM have decreased miR-381 expression and elevated COX-2 expression in peripheral blood. miR-381 may inhibit myocardial cell damage caused by CVB3 infection and protect myocardial cell function by targeting COX-2 expression. IntroductionThe incidence of viral myocarditis (VM) is gradually increased in recent years, and attracts the attention of more and more scholars (1). Coxsackievirus B3 (CVB3) is one of the most common viruses that cause VM (2). VM can develop into acute heart failure (3,4). According to available clinical studies, the mortality rate of VM in young people is as high as 21%, and sudden deaths caused by VM or fatal ventricular arrhythmias in children account for about 20% (5,6). However, there have been few effective therapies for VM by now. Current treatment for VM is still focused on the control of cardiac arrhythmia and heart failure. Therefore, it is necessary to study the molecular mechanism of VM.VM is a common infectious disease in pediatric clinical practice. It is caused by viral infection or disturbance of autoimmune system, and characterized by localized or diffuse acute or chronic inflammatory lesions of the myocardium (1,7). In inflammation, cyclooxygenase (COX), especially COX-2 in the COX family, plays an important role. COX-2 is produced after induction by external stimuli (physical...

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Section: Discussionmentioning

confidence: 99%

MicroRNA‑381 protects myocardial cell function in children and mice with viral myocarditis via targeting cyclooxygenase‑2 expression

Zhang

Sun

et al. 2018

Exp Ther Med

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“…For tumor growth, miR-381 could inhibit cell proliferation in colon cancer through targeting LRH-1 (17), in glioma through targeting BRD7 (17) and in renal cancer through targeting WEE1 (18). MiR-381 also could sensitize glioblastoma cells to temozolomide by targeting neurofilament light polypeptide (19).…”

Section: Discussionmentioning

confidence: 99%

MiR-381 inhibits migration and invasion in human gastric carcinoma through downregulatedting SOX4

Zhang¹,

Huang²,

Long³

2017

Oncology Letters

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Abstract. Aberrant expression of microRNAs (miRs) serves essential roles in the generation and progression of various types of human cancer. In the present study, the expression and biological functions of miR-381 in human gastric carcinoma (GC) were focused upon. The results of reverse transcription-quantitative polymerase chain reaction analysis revealed that the expression of miR-381 was significantly downregulated in GC tissue samples. Furthermore, low expression of miR-381 was identified to be associated with lymphatic metastasis and advanced tumor-node-metastasis stage (III+IV). Upregulation of miR-381 inhibited the migration and invasion of GC SGC-7901 cells through SRY-Box 4 (SOX4)-mediated epithelial-mesenchymal transition. Finally, long non-coding (lnc) RNA-taurine upregulatedted 1 (non-protein coding) (TUG1) was confirmed as a negatively regulator of miR-381 expression in SGC-7901 cells. Taken together, the results of the current study indicate that the downregulation of miR-381 by lncRNA-TUG1 promoted the metastasis of GC cells by inhibiting SOX4. Thus, targeting miR-381 may be a novel therapeutic option for the treatment of patients with GC.

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“…The expression of microRNA 381 (miR-381) is dysregulated in various cancer types, which suggested that miR-381 functions as oncogenic or tumor-suppressive miRNAs [4]. Some studies showed that the expression levels of miR-381 were downregulated in epithelial ovarian cancer [5, 6], hepatocellular carcinoma [7], renal carcinoma [8, 9], lung adenocarcinoma [10] and colon cancer [11], and an increased miR-381 expression inhibits the malignancy of these tumors. On the contrary, other studies indicated that the expression of miR-381 is upregulated in glioma [12] and pituitary tumors [13] and reducing miR-381 expression inhibited the proliferation and invasion of cells.…”

Section: Introductionmentioning

confidence: 99%

Low expression of miR-381 is a favorite prognosis factor and enhances the chemosensitivity of osteosarcoma

Chun-hua²,

Yu³

et al. 2016

Oncotarget

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Osteosarcoma (OS) is the most common primary bone malignancy with a poor prognosis for all races and both sexes. In this study, we found that miR-381 is a positive prognosis factor for OS patients that OS patients with a low expression of miR-381 had a longer survival time after surgical intervention, and miR-381 expression promotes MG-63 cell proliferation and cell invasion ability. Our results also showed a strong negative correlation between the expression of miR-381 and LRRC4 (brain relative specific expression gene) in OS tissues. This demonstrated that LRRC4 is a direct target gene of miR-381, and suppressing the expression of miR-381 increases the sensitivity of OS cells to chemotherapeutic drugs through the LRRC4-mediated mTOR pathway. In summary, miR-381 is an important biomarker in directing therapeutic intervention and predicting prognosis in OS patients.

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Down-regulation of MicroRNA-381 promotes cell proliferation and invasion in colon cancer through up-regulation of LRH-1

Cited by 44 publications

References 20 publications

MicroRNA‑381 protects myocardial cell function in children and mice with viral myocarditis via targeting cyclooxygenase‑2 expression

MicroRNA‑381 protects myocardial cell function in children and mice with viral myocarditis via targeting cyclooxygenase‑2 expression

MiR-381 inhibits migration and invasion in human gastric carcinoma through downregulatedting SOX4

Low expression of miR-381 is a favorite prognosis factor and enhances the chemosensitivity of osteosarcoma

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