Down‐regulation of microRNA 106b is involved in p21‐mediated cell cycle arrest in response to radiation in prostate cancer cells

Li, Baoqing; Shi, Xu; Nori, Dattatreyudu; Chao, C.; Chen, Allen M.; Valicenti, Richard K.; White, Ralph W. deVere

doi:10.1002/pros.21272

Cited by 96 publications

(82 citation statements)

References 20 publications

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“…1.0 of the GeneChip system developed by Affymetrix Inc. Total RNA was extracted from LNCaP cells 4 h after X-ray irradiation at 5 or 10 Gy. The results showed that the expression levels of many of the miRNAs were altered following X-ray irradiation, suggesting that they may be involved in the adaptive responses of cells to radiation, consistent with reports showing that miRNAs are involved in the cellular response to radiation (19).…”

Section: Resultssupporting

confidence: 89%

Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation

Morii

Ogawa

Watanabe

et al. 2013

International Journal of Molecular Medicine

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Abstract. We previously developed a promoter that was responsive to radiation by randomly combining cis-elements of transcription factors that are activated in response to radiation in prostate cancer cells. The promoter enhanced the expression of the luciferase gene linked downstream by more than 10-fold 12 h after X-ray irradiation at 10 Gy. However, without radiation, it still significantly drove its expression. To suppress expression while retaining its enhancement in response to radiation, we focused our attention on microRNAs (miRNAs). miRNAs are a group of non-coding RNAs approximately 22 nucleotides long that control gene expression by binding to a target sequence residing on the 3'-untranslated region (3'UTR) of a target gene. We identified 8 miRNAs that were downregulated in response to X-ray irradiation, and inserted artificial target sequences composed of randomly combined complementary sequences into 3 representative miRNAs into the 3'UTR of the luciferase gene. The target sequences suppressed the expression, and released the expression, after X-ray irradiation, as expected. When we combined an artificial target sequence with the radiation-responsive promoter, it resulted in a clear-cut gene regulation of expression that was greater than that induced by the promoter alone.

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Section: Resultssupporting

confidence: 89%

Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation

Morii

Ogawa

Watanabe

et al. 2013

International Journal of Molecular Medicine

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“…Nevertheless, we must mention that in most studies miR-221 and miR-222 were observed to be downregulated in human prostate cancer tissue, which clearly contradicts its described oncogenic function in prostate cancer cell lines (8 ). miR-106b, which has been shown to be part of an oncogenic miRNA cluster that regulates PTEN, has been suggested to have a regulatory effect on CDKN1A (40 ). miR-106b is suppressed upon radiation of prostate cancer cell lines, and overexpression of this miRNA can inhibit radiationmediated CDKN1A activation, thereby overcoming subsequent arrest of the cell cycle.…”

Section: Prostate Cancermentioning

confidence: 88%

MicroRNAs as Regulators of Signal Transduction in Urological Tumors

et al. 2011

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BACKGROUND: MicroRNAs (miRNAs) are short noncoding RNAs that have been shown to play pivotal roles in carcinogenesis. In the past decade, miRNAs have been the focus of much research in oncology, and there are great expectations for their utility as cancer biomarkers and therapeutic targets.CONTENT: In this review we examine how miRNAs can regulate signal transduction pathways in urological tumors. We performed in silico target prediction using TargetScan 5.1 to identify the signal transduction targets of miRNA, and we summarize the experimental evidence detailing miRNA regulation of pathways analyzed herein.

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“…In prostate cancer, multiple miRNAs, including miR106b, have demonstrated altered expression in response to radiation [119] . The up-regulation of miR-106b in prostate cancer overrides p21-activated cell cycle arrest and growth inhibition induced by radiation [119] , providing a potential target for radiation-resistant prostate cancer. In contrast to miR-106b, miR-101 sensitizes tumor cells to radiation in vitro and in vivo by targeting DNA-PKCs and ATM [120] .…”

Section: Implications Of Cell Cycle-related Mirnas In Anti-cancer Thementioning

confidence: 99%

Macro-management of microRNAs in cell cycle progression of tumor cells and its implications in anti-cancer therapy

Liang

2011

Acta Pharmacol Sin

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The cell cycle, which is precisely controlled by a number of regulators, including cyclins and cyclin-dependent kinases (CDKs), is crucial for the life cycle of mammals. Cell cycle dysregulation is implicated in many diseases, including cancer. Recently, compelling evidence has been found that microRNAs play important roles in the regulation of cell cycle progression by modulating the expression of cyclins, CDKs and other cell cycle regulators. Herein, the recent findings on the regulation of the cell cycle by microRNAs are summarized, and the potential implications of miRNAs in anti-cancer therapies are discussed.

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Down‐regulation of microRNA 106b is involved in p21‐mediated cell cycle arrest in response to radiation in prostate cancer cells

Cited by 96 publications

References 20 publications

Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation

Utilization of microRNAs with decreased expression levels in response to X-ray irradiation for fine-tuning radiation-controlled gene regulation

MicroRNAs as Regulators of Signal Transduction in Urological Tumors

Macro-management of microRNAs in cell cycle progression of tumor cells and its implications in anti-cancer therapy

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