Down-regulation of genes encoding steroidogenic enzymes and hormone receptors in late preovulatory follicles of the cow coincides with an accumulation of intrafollicular steroids

Nimz, Marika; Spitschak, Marion; Schneider, Falk; Fürbaß, Rainer; Vanselow, Jens

doi:10.1016/j.domaniend.2009.02.002

Cited by 46 publications

(62 citation statements)

References 25 publications

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“…Increased PGR (Natraj & Richards 1993) and CYP11A1 (Rao et al 1978) expression has been reported in rat luteinized GCs. Studies in humans and in the bovine suggest that LH surge-induced declines of FSHR represent the initiation of GC luteinization (Nimz et al 2009, Jeppesen et al 2012. Likewise, disappearance of CYP19A1 is another marker of luteinization in GCs (Campbell et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Aging-related premature luteinization of granulosa cells is avoided by early oocyte retrieval

Wu¹,

Barad²,

Kushnir³

et al. 2015

Journal of Endocrinology

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Why IVF pregnancy rates decline sharply after age 43 is unknown. In this study, we compared granulosa cell (GC) function in young oocyte donors (nZ31, ages 21-29), middle-aged (nZ64, ages 30-37) and older infertile patients (nZ41, ages 43-47). Gene expressions related to gonadotropin activity, steroidogenesis, apoptosis and luteinization were examined by real-time PCR and western blot in GCs collected from follicular fluid. FSH receptor (FSHR), aromatase (CYP19A1) and 17b-hydroxysteroid dehydrogenase (HSD17B) expression were found down regulated with advancing age, while LH receptor (LHCGR), P450scc (CYP11A1) and progesterone receptor (PGR) were up regulated. Upon in vitro culture, GCs were found to exhibit lower proliferation and increased apoptosis with aging. While FSH supplementation stimulated GCs growth and prevented luteinization in vitro. These observations demonstrate age-related functional declines in GCs, consistent with premature luteinization. To avoid premature luteinization in women above age 43, we advanced oocyte retrieval by administering human chorionic gonadotropin at maximal leading follicle size of 16 mm (routine 19-21 mm). Compared to normal cycles in women of similar age, earlier retrieved patients demonstrated only a marginal increase in oocyte prematurity, yet exhibited improved embryo numbers as well as quality and respectable clinical pregnancy rates. Premature follicular luteinization appears to contribute to rapidly declining IVF pregnancy chances after age 43, and can be avoided by earlier oocyte retrieval.

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Section: Discussionmentioning

confidence: 99%

Aging-related premature luteinization of granulosa cells is avoided by early oocyte retrieval

Wu¹,

Barad²,

Kushnir³

et al. 2015

Journal of Endocrinology

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“…We showed in the current study for the first time that assessing the rate of CL development after the taking of follicle fluid samples from a pre-ovulatory follicle, allows discrimination between follicles with different qualities, which might be taken as the same group if they were judged commonly on the basis of oestradiol and progesterone concentrations (Lohrke et al 2005, Nishimoto et al 2009 or morphology (Acosta 2007). Both groups of pre-ovulatory follicles (with or without subsequent development of a corpus luteum) showed expected, or even higher, progesterone concentrations in their FF (Lohrke et al 2005;Nimz et al 2009). However, our results indicate that the oestradiol concentration is more important for predicting the further physiological development of a pre-ovulatory follicle into luteal tissue and this might fit with recent in vivo findings regarding plasma oestradiol and pregnancy rates (Jinks et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Vernunft¹,

Weitzel²,

Viergutz³

2013

Vet. Med. - Czech

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Secretion of adequate levels of progesterone from a proper corpus luteum (CL), which develops out of the cells of a healthy preovulatory follicle, is a key-factor for establishment of a pregnancy. The aim of this study was to investigate the relationship between morphological and secretory characteristics of preovulatory follicles and their corresponding corpus luteum with regard to the post-partum period in high-yielding dairy cows. Therefore, ultrasound-guided aspirations of preovulatory follicles were performed repeatedly, using 20 first lactating cows between 26 and 121 days after parturition. Heat was induced with a PGF analogon followed by administration of a GnRH analogon. The dominant follicle was aspirated 21 h after administration of the GnRH analogon. The diameters of the follicles were estimated at aspiration and the morphology of the resulting luteal tissue was examined on day 14 after follicle aspiration using ultrasonographic examinations. Concentrations of progesterone (P 4 ) and 17-beta-oestradiol (E 2 ) were determined in the follicular fluids (FF) and P 4 concentration was estimated at the time of CL examination in plasma. A CL development occurred in 82% after dominant follicle aspiration. The interval of time between parturition and follicle aspiration did not affect the investigated variables. The diameter of the aspirated preovulatory follicle was positively correlated to the cross-section area of the developed luteal tissue (R = 0.60; P < 0.01) as well as to the plasma P 4 concentration on day 14 after follicle aspiration (R = 0.47; P < 0.05). Also, E 2 concentrations in FF were positively correlated to cross-section area of the luteal tissue (R = 0.54; P < 0.05). Comparing the FF of the follicles that gave rise to a CL after aspiration to follicles that did not, both types had comparable P 4 , but the former type harboured higher E 2 concentrations. In conclusion, preovulatory follicle diameter as well as steroid concentrations in the follicular fluid could be used prospectively to identify cows which will have well-developed CLs and high plasma P 4 levels later. On the other hand, CL development after follicle aspiration can be used as a retrospective quality parameter of dominant follicles. These results will help to identify suitable animals for breeding or recipients for embryo transfer.Keywords: post-partum period; ultrasound; fertility; progesterone; oestradiol; follicle fluid List of abbreviations CL = corpus luteum, DM = dry matter, E 2 = 17-beta-oestradiol, FF = follicle fluid, GnRH = gonadotropin realising hormone, P 4 = progesterone, PGF = prostaglandin F2 alfa, TMR = total mixed ration

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“…The cellular mechanisms regulating folliculogenesis, ovulation, and follicular regression in cows are not fully defined. However, a key role of gonadotropins in the regulation of follicular development has been well established (Nogueira et al 2007, Nimz et al 2009). In normal ovaries, the interactive system of follicular growth and steroidogenesis suggests that granulosa and theca cells are involved in the secretion of steroid hormones through the two-cell/two-gonadotropin model (Fortune & Quirk 1988).…”

Section: Expression Of Gonadotropin Receptorsmentioning

confidence: 99%

“…The distribution pattern and expression levels of gonadotropin receptors in healthy ovaries have been evaluated by several methods, including conventional and real-time RT-PCR, northern blot assays, and in situ hybridization (Ireland & Roche 1983, Xu et al 1995, Bodensteiner et al 1996, Rajapaksha et al 1996, Bao et al 1997, Evans & Fortune 1997, Bao & Garverick 1998, Soumano et al 1998, Odore et al 1999, Calder et al 2001, Manikkam et al 2001, Robert et al 2003, Braw-Tal & Roth 2005, Luo & Wiltbank 2006, Mihm et al 2006, Nogueira et al 2007, Nimz et al 2009). In recent studies conducted in our laboratory, we analyzed the relative expression levels of LHCGR and FSHR in follicular cysts of cows with COD and antral follicles of healthy animals by real-time RT-PCR and, in agreement with that found by other authors, we found that mRNA expression of FSHR in antral follicles was exclusively localized to granulosa cells and it decreased as follicle size increased, and that LHCGR mRNA was detected in theca cells of antral follicles of all sizes and in the granulosa cells of large antral follicles, being theca cells of medium antral follicles, the ones that showed the highest expression (Marelli et al 2014).…”

Section: Expression Of Gonadotropin Receptorsmentioning

confidence: 99%

Molecular aspects of bovine cystic ovarian disease pathogenesis

Ortega¹,

Marelli²,

Rey³

et al. 2015

Reproduction

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Cystic ovarian disease (COD) is one of the main causes of reproductive failure in cattle and causes severe economic loss to the dairy farm industry because it increases both days open in the post partum period and replacement rates due to infertility. This disease is the consequence of the failure of a mature follicle to ovulate at the time of ovulation in the estrous cycle. This review examines the evidence for the role of altered steroid and gonadotropin signaling systems and the proliferation/apoptosis balance in the ovary with cystic structures. This evidence suggests that changes in the expression of ovarian molecular components associated with these cellular mechanisms could play a fundamental role in the pathogenesis of COD. The evidence also shows that gonadotropin receptor expression in bovine cystic follicles is altered, which suggests that changes in the signaling system of gonadotropins could play a fundamental role in the pathogenesis of conditions characterized by altered ovulation, such as COD. Ovaries from animals with COD exhibit a disrupted steroid receptor pattern with modifications in the expression of coregulatory proteins. These changes in the pathways of endocrine action would trigger the changes in proliferation and apoptosis underlying the aberrant persistence of follicular cysts. Free Spanish abstract: A Spanish translation of this abstract is freely available at

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Down-regulation of genes encoding steroidogenic enzymes and hormone receptors in late preovulatory follicles of the cow coincides with an accumulation of intrafollicular steroids

Cited by 46 publications

References 25 publications

Aging-related premature luteinization of granulosa cells is avoided by early oocyte retrieval

Aging-related premature luteinization of granulosa cells is avoided by early oocyte retrieval

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Molecular aspects of bovine cystic ovarian disease pathogenesis

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