Down-regulation of FUBP1 is related to intervertrbral disc degeneration through NRF2 signalling pathway

Abstract

Aim: Herein, we plan to explore the potential mechanism between intervertebral disc degeneration (IDD) and FUBP1 in reactive oxygen species mitochondria. Methods: IDD samples were collected from our hospital and applied in our study. Firstly, the expression and function of FUBP1 were detected in IDD samples and cells by qRT-PCR and in vivo assays. Then, the downstream gene of FUBP1 was explored and verified in functional experiments. Finally, the whole mechanism of FUBP1 in IDD was elucidated clearly. Results:FUBP1 was found to be dysregulated in early and advanced stages of IDD, and its sh-FUBP1 suppressed NP cell proliferation, while over-FUBP1 promoted cell proliferation in vitro and in vivo. Then, NRF2 was considered a target gene of FUBP1 in IDD, which was confirmed in experiments. FUBP1 had a positive relation with NRF2 in IDD. Conclusion: In this study, we mainly make an investigation on FUBP1 and IDD progression based on the early and advanced IDD samples and a series of functional assays. It is concluded that FUBP1 could weaken the progression of IDD by targeting NRF2, which gives directions for the clinical therapy of IDD patients.