Down-regulation of fatty acid synthase increases the resistance of Saccharomyces cerevisiae cells to H2O2

Matias, Ana Catarina; Pedroso, Nuno M.; Teodoro, Nuno; Marinho, H. Susana; Antunes, Fernando; Nogueira, J.M.F.; Herrero, Enrique; Cyrne, Luísa

doi:10.1016/j.freeradbiomed.2007.08.003

Cited by 30 publications

(20 citation statements)

References 42 publications

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“…Possibly, the predicted decrease in the saturation levels of fatty acids, and therefore the increase in membrane permeability act as part of a stress-response mechanism to facilitate export of compatible solutes when they are no longer needed [57]. Recent studies in S. cerevisiae indicate that H 2 O 2 challenge down-regulates the expression of genes encoding enzymes involved in lipid metabolism including those for fatty acid synthesis that lead to a reorganization of the plasma membrane and a lower permeability to H 2 O 2 [58], [59]. However, our results revealed the opposite expression pattern for those genes, indicating increased permeability of H 2 O 2 caused by ionizing radiation.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of the Black Yeast Wangiella dermatitidis to Ionizing Radiation: Molecular and Cellular Mechanisms

et al. 2012

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Observations of enhanced growth of melanized fungi under low-dose ionizing radiation in the laboratory and in the damaged Chernobyl nuclear reactor suggest they have adapted the ability to survive or even benefit from exposure to ionizing radiation. However, the cellular and molecular mechanism of fungal responses to such radiation remains poorly understood. Using the black yeast Wangiella dermatitidis as a model, we confirmed that ionizing radiation enhanced cell growth by increasing cell division and cell size. Using RNA-seq technology, we compared the transcriptomic profiles of the wild type and the melanin-deficient wdpks1 mutant under irradiation and non-irradiation conditions. It was found that more than 3000 genes were differentially expressed when these two strains were constantly exposed to a low dose of ionizing radiation and that half were regulated at least two fold in either direction. Functional analysis indicated that many genes for amino acid and carbohydrate metabolism and cell cycle progression were down-regulated and that a number of antioxidant genes and genes affecting membrane fluidity were up-regulated in both irradiated strains. However, the expression of ribosomal biogenesis genes was significantly up-regulated in the irradiated wild-type strain but not in the irradiated wdpks1 mutant, implying that melanin might help to contribute radiation energy for protein translation. Furthermore, we demonstrated that long-term exposure to low doses of radiation significantly increased survivability of both the wild-type and the wdpks1 mutant, which was correlated with reduced levels of reactive oxygen species (ROS), increased production of carotenoid and induced expression of genes encoding translesion DNA synthesis. Our results represent the first functional genomic study of how melanized fungal cells respond to low dose ionizing radiation and provide clues for the identification of biological processes, molecular pathways and individual genes regulated by radiation.

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Section: Discussionmentioning

confidence: 99%

Adaptation of the Black Yeast Wangiella dermatitidis to Ionizing Radiation: Molecular and Cellular Mechanisms

et al. 2012

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“…Phenotypes listed in Table 1 as oxidative stress-related consist largely of sensitivity to an oxidizing agent such as peroxide, in the case of ypc1 Δ (Higgins et al, 2002), sur2 Δ (Berry et al, 2011), csg1 Δ (Berry et al, 2011), isc1 Δ (Almeida et al, 2008; Berry et al, 2011; Chang et al, 2002), and elo2 Δ (Berry et al, 2011), or MMS as in ypc1 Δ (Begley et al, 2002), scs7 Δ (Begley et al, 2002), and isc1 Δ (Chang et al, 2002; Matmati et al, 2009). There are also cases of resistance phenotypes such as the fas1 Δ strain (deleted in the fatty acid synthetase gene FAS1) resistance to hydrogen peroxide (Matias et al, 2007), and the reported increased genomic stability of lac1 Δ when challenged with the non-proteogenic amino acid canavanine (Alabrudzinska et al, 2011). The elo1 Δ and elo2 Δ strains also show ROS accumulation and oxidative stress sensitivity, when transformed with the human α-synuclein protein as part of a Parkinson’s disease model (Lee et al, 2011).…”

Section: Phenotypes Of the Yeast Sphingolipid Pathwaymentioning

confidence: 99%

The yeast sphingolipid signaling landscape

Montefusco

Matmati

Hannun

2014

Chemistry and Physics of Lipids

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Sphingolipids are recognized as signaling mediators in a growing number of pathways, and represent potential targets to address many diseases. The study of sphingolipid signaling in yeast has created a number of breakthroughs in the field, and has the potential to lead future advances. The aim of this article is to provide an inclusive view of two major frontiers in yeast sphingolipid signaling. In the first section, several key studies in the field of sphingolipidomics are consolidated to create a yeast sphingolipidome that ranks nearly all known sphingolipid species by their level in a resting yeast cell. The second section presents an overview of most known phenotypes identified for sphingolipid gene mutants, presented with the intention of illuminating not yet discovered connections outside and inside of the field.

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“…ise çalışmalarında S. cerevisiae'nın plazma membranlarının H 2 O 2 'ye maruz kaldıktan birkaç dakika sonra hızlı bir şekilde geçirgenlik ve biyofizyolojik özelliklerini değiştirdiğini gözlemlemiş ve bu değişikliklerin lipit bileşimindeki değişmelerden kaynaklandığını ileri sürmüştür [13]. Hidrojen peroksite karşı hücre zarının potansiyel geçirgenliği üzerine yağ asit sentetaz geni tarafından kodlanan yağ asidi sentezinin rolü incelenmiştir [14]. Maya hücresinin H 2 O 2 'ye karşı yağ asidi sentaz aktivitesini arttırdığı, membran yağ kompozisyonda ki bu artış hücrenin H 2 O 2 geçirgenliğine direnç göstermesi ve canlılık halinin uzamasına neden olduğu sonucuna varılmıştır [14].…”

Section: Materyal Ve Yöntemunclassified

“…Hidrojen peroksite karşı hücre zarının potansiyel geçirgenliği üzerine yağ asit sentetaz geni tarafından kodlanan yağ asidi sentezinin rolü incelenmiştir [14]. Maya hücresinin H 2 O 2 'ye karşı yağ asidi sentaz aktivitesini arttırdığı, membran yağ kompozisyonda ki bu artış hücrenin H 2 O 2 geçirgenliğine direnç göstermesi ve canlılık halinin uzamasına neden olduğu sonucuna varılmıştır [14]. Şekil 1.…”

Section: Materyal Ve Yöntemunclassified

Plumbago europaea L.’nın Besi Yeri Ortamındaki Saccharomyces cerevisiae Gelişimi Üzerine Etkileri

Bircan¹,

Kırbağ²

2015

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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ÖzetPlumbaginaceae ailesinin bir üyesi olan P. europea, antibakteriyal, antifungal, antikanser ve antimutajenik özellik gösteren medikal bir bitkidir. Bu çalışma S. cerevisiae kültür ortamında oluşturulan oksidatif strese karşı P. europea özütünün antioksidan etkilerini belirlemek amacıyla yapıldı. Çalışmada kullanılan S. cerevisiae besiyerinde çoğaltıldı ve oksidatif stres oluşturmak amacıyla ortama H 2 O 2 + FeCl 2 (bitki grubuna H 2 O 2 + FeCl 2 ilave olarak P. europea özütü (PLM)) eklendi. P. europea'nın maya kültür ortamında oluşturulan oksidadtif strese karşı koruyucu etkisi malondialdehit (MDA) seviyesi, glutatyon, total protein ve süperoksit dismütaz (SOD) düzeylerini ölçülerek belirlendi. Bitki özütü eklenen grupta MDA ve total protein seviyeleri reaktif grubuna (FR) kıyasla arttı ancak glutatyon miktarı anlamlı seviyede azaldı (p<0.05). Sodyum dodesil sülfatlı-poliakrilamit jel elektroforezi (SDS-Page) analizinde ise SOD ve katalaz (CAT) miktarının FR grubuna kıyasla azaldığı tespit edildi. Çalışma sonuçlarımız P. europea'nın maya kültürü üzerine oksidan etki gösterdiğini ortaya koymaktadır. Bu etkinin nasıl meydana geldiği veya maya üzerine hangi bileşiklerin toksik etki gösterdiğini belirlemek için kapsamlı araştırmalara ihtiyaç vardır. Anahtar Kelimeler: Plumbago europea, Saccharomyces cerevisiae, antioksidan aktivite, toplam protein The Effect of Plumbago europaea L. on Saccharomyces cerevisiae in Medium AbstractThe P. europea is a medical plant having antibacterial, antifungal, anticancer and antimutagen characteristic as a member of Plumbaginacea family. This study was achieved to determine the antioxidant effects of the P. europea extract against oxidative stress to be formed in S. cerevisiae media. The S. cerevisiae that was used in the study, was proliferated in the medium and the media was added H 2 O 2 + FeCl 2 (to the plant group H 2 O 2 + FeCl 2 plus P. europea extract (PLM)) to form the oxidative stress. The protective effect of the P. europea against oxidative stress to be formed in yeast culture media was determined by measuring the levels of malondialdehyde (MDA), glutathione, total protein and superoxide dismutase (SOD). The levels of MDA and total protein in the group that was added plant extract, with compare to the reactive group (FR), however the amount of the glutathione significantly decreased (p<0.05). The sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that the SOD and catalase (CAT) amounts were seen to be reduced with compare the FR group. The conclusions of our study indicate that P. europea has oxidant effects on yeast culture. There are more comprehensive studies to be done to explain how this effect occur or which compounds have toxic effects on the yeast.

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Down-regulation of fatty acid synthase increases the resistance of Saccharomyces cerevisiae cells to H2O2

Cited by 30 publications

References 42 publications

Adaptation of the Black Yeast Wangiella dermatitidis to Ionizing Radiation: Molecular and Cellular Mechanisms

Adaptation of the Black Yeast Wangiella dermatitidis to Ionizing Radiation: Molecular and Cellular Mechanisms

The yeast sphingolipid signaling landscape

Plumbago europaea L.’nın Besi Yeri Ortamındaki Saccharomyces cerevisiae Gelişimi Üzerine Etkileri

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