Down-regulation of experimental allergic encephalomyelitis in DA rats by tiazofurin

Stošić‐Grujičić, Stanislava; Savić-Radojević, Ana; Maksimović‐Ivanić, Danijela; Marković, Miloš; Bumbaširević, Vladimir; Ramić, Zorica; Mostarica-Stojković, Marija

doi:10.1016/s0165-5728(02)00210-2

Cited by 17 publications

(21 citation statements)

References 36 publications

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“…Another possibility, not mutually exclusive, is that ribavirin affects migration of encephalitogenic T lymphocytes to the target tissue [40]. This effect is consistent with the findings that GTP deprivation by IMPDH inhibitors suppresses glycosylation and the binding activity of various adhesion molecules [34,41,42], participating in regulation of lymphocyte migratory pattern.…”

Section: Discussionsupporting

confidence: 81%

Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production

Lavrnja

Stojkov

Bjelobaba

et al. 2008

International Immunopharmacology

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Section: Discussionsupporting

confidence: 81%

Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production

Lavrnja

Stojkov

Bjelobaba

et al. 2008

International Immunopharmacology

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“…Cervical lymph node cells (CLNC) were isolated from untreated AO rats, Dark Agouti (DA) rats, or (AO 3 DA) F1 hybrids, as previously described (Stosic-Grujicic et al, 2002). For the experiments investigating antigen-specific production of cytokines, DA rats were injected intradermally (i.d.)…”

Section: Materials and Methods Astrocytes And Lymph Node Cellsmentioning

confidence: 99%

Astrocytes stimulate interleukin‐17 and interferon‐γ production in vitro

Miljković¹,

Momčilović²,

Stojanović³

et al. 2007

J of Neuroscience Research

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Astrocytes play important roles in the complex and as yet not very well understood net of interactions among resident and infiltrating cells during central nervous system (CNS) inflammation. In such an intricate network, cytokines represent an essential means for intercellular communication, and astrocytes are able to affect their generation and/or release. Among various cytokines produced by infiltrating cells, interferon (IFN)-gamma and interleukin (IL)-17 are the focus of this research, because they are pivotal cytokines of helper T-cell type 1 (Th1) and helper T-cell type 17 (Th17), respectively. Importantly, both Th1 and Th17 cells, as well as their cytokines, have been shown to be of importance for the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model of a prototypical CNS disease with inflammatory pathogenesis, multiple sclerosis. Therefore, the influence of astrocytes on the generation of IFN-gamma and IL-17 in concanavalin A- and myelin basic protein-stimulated lymph node cells of healthy rats and rats with developing EAE, respectively, was investigated in vitro. Astrocytes up-regulated IL-17 and IFN-gamma gene expression and protein synthesis in T cells, which coincided with astrocytes' ability to express IL-23 subunit p19 and common IL-12/IL-23 subunit p40 but not IL-12 subunit p35 in the co-cultivations. These results suggest one more way in which astrocytes could contribute to the complex interactions during CNS inflammation.

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“…The data were plotted as daily mean clinical score for all animals in a particular treatment group. Several parameters of disease were examined to evaluate the severity of EAE and the efficacy of BA therapy that include mean clinical score, incidence, mean day of onset, peak clinical score, and cumulative disease index (8).…”

Section: Clinical Evaluationmentioning

confidence: 99%

Baicalin reduces the severity of experimental autoimmune encephalomyelitis

Zeng

Song

Xin-sheng

et al. 2007

Braz J Med Biol Res

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Scutellaria baicalensis Georgi is one of the important medicinal herbs widely used for the treatment of various inflammatory diseases in Asia. Baicalin (BA) is a bioactive anti-inflammatory flavone found abundantly in Scutellaria baicalensis Georgi. To explore the therapeutic potential of BA, we examined the effects of systemic administration of the flavone (5 and 10 mg/kg, ip) on relapsing/remitting experimental autoimmune encephalomyelitis (EAE) induced by proteolipid protein 139-151 in SJL/J mice, an experimental model of multiple sclerosis. The mice treated with PBS or BA at day -1 and for 3 consecutive days were observed daily for clinical signs of disease up to 60 days after immunization. In the PBS-EAE group, neurological scores were: incidence (100%), mean day of onset (8.0 ± 0.73), peak clinical score (3.0 ± 0.4), and cumulative disease index (141.8 ± 19.4). In the BA-EAE group (5 or 10 mg kg -1 day -1 , respectively), incidence (95 or 90%), mean day of onset (9.0 ± 0.80 or 9.2 ± 0.75; P = 0.000), peak clinical score (2.2 ± 0.3 or 2.0 ± 0.3; P = 0.000), and cumulative disease index (75.9 ± 10.1 or 62.9 ± 8.4; P = 0.000) decreased, accompanied by the histopathological findings (decrease of dense mononuclear infiltration surrounding vascellum) for the spinal cord. Additionally, the in vitro effects of BA (5, 10, and 25 µM) on mononuclear cells collected from popliteal and inguinal lymph nodes of day-10 EAE mice were evaluated using an MTT reduction assay for cell proliferation, and ELISA to measure IFN-γ and IL-4 cytokines. Compared with the control group, BA caused an increase in IL-4 (EAE-DMSO: 3.56 ± 0.42 pg/mL vs EAE-BA (5, 10, and 25 µM): 6.03 ± 1.1, 7.83 ± 0.65, 10.54 ± 1.13 pg/mL, respectively; P < 0.001); but inhibited IFN-γ (EAE-DMSO: 485.76 ± 25.13 pg/mL vs EAE-BA (5, 10, and 25 µM): 87.08 ± 9.24, 36.27 ± 5.44, 19.18 ± 2.93 pg/mL, respectively; P < 0.001) and the proliferation of mononuclear cells (EAE-DMSO: 0.73 ± 0.021 vs EAE-BA (5, 10, and 25 µM): 0.41 ± 0.015, 0.31 ± 0.018, 0.21 ± 0.11, respectively; P < 0.001) in a concentration-dependent manner. The results suggest that BA might be effective in the treatment of multiple sclerosis.

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Down-regulation of experimental allergic encephalomyelitis in DA rats by tiazofurin

Cited by 17 publications

References 36 publications

Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production

Ribavirin ameliorates experimental autoimmune encephalomyelitis in rats and modulates cytokine production

Astrocytes stimulate interleukin‐17 and interferon‐γ production in vitro

Baicalin reduces the severity of experimental autoimmune encephalomyelitis

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