Down‐regulation of Cinnamoyl‐CoA Reductase induces significant changes of lignin profiles in transgenic tobacco plants

Piquemal, Joël; Lapierre, Catherine; Myton, Kate; O’Connell, Ann P.; Schuch, Wolfgang; Grima‐Pettenati, Jacqueline; Boudet, Alain‐Michel

doi:10.1046/j.1365-313x.1998.00014.x

Cited by 304 publications

(257 citation statements)

References 50 publications

Supporting

Mentioning

233

Contrasting

Unclassified

Order By: Relevance

“…It is noteworthy that, in addition to HCT itself, genes directly downstream of HCT, namely C3H, CCR, and CCoAOMT, are those whose repression has been shown to have the strongest impact on plant growth (Piquemal et al, 1998;Jones et al, 2001;Pinç on et al, 2001aPinç on et al, , 2001bFranke et al, 2002;Goujon et al, 2003;Abdulrazzak et al, 2005;Patten et al, 2005). HCT repression had a particularly strong impact on growth, likely due to its double role in lignin biosynthesis upstream and downstream of the C3H step (see Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Flavonoid Accumulation in Arabidopsis Repressed in Lignin Synthesis Affects Auxin Transport and Plant Growth

et al. 2007

Self Cite

View full text Add to dashboard Cite

In Arabidopsis thaliana, silencing of hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), a lignin biosynthetic gene, results in a strong reduction of plant growth. We show that, in HCT-silenced plants, lignin synthesis repression leads to the redirection of the metabolic flux into flavonoids through chalcone synthase activity. Several flavonol glycosides and acylated anthocyanin were shown to accumulate in higher amounts in silenced plants. By contrast, sinapoylmalate levels were barely affected, suggesting that the synthesis of that phenylpropanoid compound might be HCTindependent. The growth phenotype of HCT-silenced plants was shown to be controlled by light and to depend on chalcone synthase expression. Histochemical analysis of silenced stem tissues demonstrated altered tracheary elements. The level of plant growth reduction of HCT-deficient plants was correlated with the inhibition of auxin transport. Suppression of flavonoid accumulation by chalcone synthase repression in HCT-deficient plants restored normal auxin transport and wildtype plant growth. By contrast, the lignin structure of the plants simultaneously repressed for HCT and chalcone synthase remained as severely altered as in HCT-silenced plants, with a large predominance of nonmethoxylated H units. These data demonstrate that the reduced size phenotype of HCT-silenced plants is not due to the alteration of lignin synthesis but to flavonoid accumulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Flavonoid Accumulation in Arabidopsis Repressed in Lignin Synthesis Affects Auxin Transport and Plant Growth

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…Most studies on genetic modification of lignin biosynthesis in transgenic plants have utilized the cauliflower mosaic virus 35S promoter to drive expression of sense or antisense lignification-associated genes (Halpin et al, 1994;Ni et al, 1994;Atanassova et al, 1995;Doorsselaere et al, 1995;Piquemal et al, 1998;Zhong et al, 1998;Baucher et al, 1999). However, antisense downregulation of COMT and CCOMT in transgenic alfalfa is inefficient and relatively weak using 35S promoter constructs (V.J.H.…”

Section: Cell Type Specificity Of the Bean Pal2 Promoter In Transgenimentioning

confidence: 99%

Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin

et al. 2001

View full text Add to dashboard Cite

“…The primary practical goals of these research efforts are to develop forage crops that have more digestible cell walls for animal feeding and trees that have improved pulping characteristics for paper production. Several reports on lignin transgenic plants have noted abnormal growth and developmental characteristics (Lee et al, 1997;Piquemal et al, 1998;Ralph et al, 1998b;Tamagnone et al, 1998). In a recent commentary on genetic modification of lignification, Jung and Ni (1998) concluded that when transgenic plants exhibited significant reductions in lignin content some abnormal growth and development were observed.…”

Section: Ligninmentioning

confidence: 99%

Genetic Modification of Herbaceous Plants for Feed and Fuel

Vogel

Jung

2001

Critical Reviews in Plant Sciences

View full text Add to dashboard Cite

Abstract:Much of the research on the genetic modification of herbaceous plant cell walls has been conducted to improve the utilization of forages by ruminant livestock. The rumen of these animals is basically an anaerobic fermentation vat in which the microflora break down the complex polysaccharides of plant cell walls into simpler compounds that can be further digested and absorbed by the mammalian digestive system. Research on improving the forage digestibility of switchgrass, Panicum virgatum L., and other herbaceous species has demonstrated that genetic improvements can be made in forage quality that can have significant economic value. To meet future energy needs, herbaceous biomass will need to be converted into a liquid fuel, probably ethanol, via conversion technologies still under development. If feedstock quality can be genetically improved, the economics and efficiency of the conversion processes could be significantly enhanced. Improving an agricultural product for improved end product use via genetic modification requires knowledge of desired quality attributes, the relative economic value of the quality parameters in relation to yield, genetic variation for the desired traits, or for molecular breeding, knowledge of genes to suppress or add, and knowledge of any associated negative consequences of genetic manipulation. Because conversion technology is still under development, desirable plant feedstock characteristics have not been completely delineated. Some traits such as cellulose and lignin concentration will undoubtably be important. Once traits that affect biomass feedstock conversion are identified, it will be highly feasible to genetically modify the feedstock quality of herbaceous plants using both conventional and molecular breeding techniques. The use of molecular markers and transformation technology will greatly enhance the capability of breeders to modify the morphologic structure and cell walls of herbaceous species. It will be necessary to monitor gene flow to remnant wild populations of biomass plants and have strategies available to curtail gene flow if it becomes a potential problem. It will also be necessary to monitor plant survival and longterm productivity as affected by these genetic changes to herbaceous species.

show abstract

Down‐regulation of Cinnamoyl‐CoA Reductase induces significant changes of lignin profiles in transgenic tobacco plants

Cited by 304 publications

References 50 publications

Flavonoid Accumulation in Arabidopsis Repressed in Lignin Synthesis Affects Auxin Transport and Plant Growth

Flavonoid Accumulation in Arabidopsis Repressed in Lignin Synthesis Affects Auxin Transport and Plant Growth

Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin

Genetic Modification of Herbaceous Plants for Feed and Fuel

Contact Info

Product

Resources

About