Down-Regulating CsHT1, a Cucumber Pollen-Specific Hexose Transporter, Inhibits Pollen Germination, Tube Growth, and Seed Development

Cheng, Jintao; Wang, Zhenyu; Yao, Fengzhen; Gao, Lihong; Ma, Shen; Sui, Xiaolei; Zhang, Zhenxian

doi:10.1104/pp.15.00290

Cited by 64 publications

(70 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reduction in number of pollen and their non‐viability in QPT ‐silenced transformants might also be due to severe reduction of pyridine nucleotide pool as suggested by the earlier results of PAL gene silencing (Elkind et al , Fischer et al ). A similar response was seen earlier when antisense of CsHT1 arrested pollen germination, pollen tube growth and seed development in transgenic cucumber plants (Cheng et al ). The difference in the size, shape and length of the stigma and style might also be due to the reduction pyridine nucleotide pool in keeping with their reversible role of pyridine nucleotides proposed in pollen growth and development (Hashida et al ).…”

Section: Discussionsupporting

confidence: 76%

Cloning and functional characterization of quinolinic acid phosphoribosyl transferase (QPT) gene of Nicotiana tabacum

Khan

Pandey

Jyotshna

et al. 2017

Physiologia Plantarum

View full text Add to dashboard Cite

The quinolinate phosphoribosyl transferase (QPT) is a key enzyme that converts quinolinic acid into nicotinic acid mononucleotide. The QPT gene plays an essential role in the pyridine nucleotide cycle as well as in the biosynthetic pathway of the alkaloid nicotine. However, a clear role for QPT is yet to be characterized to validate the actual function of this gene in planta. In this study, an RNA interference (RNAi) approach was used to reveal the functional role of QPT. Transformation and analysis of the hairy roots (HRs) of the Nicotiana leaf explants was used, followed by plant regeneration and analysis. High-performance liquid chromatography (HPLC) analysis of the HRs and of the regenerated plants both revealed altered alkaloid biosynthetic cycle, with a substantially reduced content of nicotine and anabasine. The transgenic plants exhibited a significantly altered phenotype and growth pattern. Also, silencing of QPT led to a decrease in chlorophyll content, maximum quantum efficiency of PSII, net CO assimilation and starch content. Results clearly demonstrated that QPT was not only involved in the biosynthetic pathway of the alkaloids but also affected plant growth and development. Our results provide information to be considered when trying to engineer the secondary metabolite quality and quantity.

show abstract

Section: Discussionsupporting

confidence: 76%

Cloning and functional characterization of quinolinic acid phosphoribosyl transferase (QPT) gene of Nicotiana tabacum

Khan

Pandey

Jyotshna

et al. 2017

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…For cucumber plant transformation, constructs were transformed into the cucumber pure line Xintai Mici as previously described (Cheng et al, 2015). Agrobacterium LB4404 harboring the constructs were used for cucumber transformation.…”

Section: Methodsmentioning

confidence: 99%

Cucumber CsBPCs Regulate the Expression of CsABI3 during Seed Germination

Liu

Bai

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

Cucumber seeds with shallow dormancy start to germinate in fruit that are harvested late. ABSCISIC ACID INSENSITIVE3 (ABI3), a transcription factor in the abscisic acid (ABA) signaling pathway, is one of the most important regulators in the transition from late embryogenesis to germination. Our analysis found a candidate cis-regulatory motif for cucumber BASIC PENTACYSTEINE (CsBPC) in the promoter of CsABI3. Yeast one-hybrid and chromatin immunoprecipitation (ChIP) assays showed that CsBPCs bound to the promoter of CsABI3. Examination of β-glucuronidase (GUS) activity driven by the CsABI3 promoter in transgenic Arabidopsis thaliana plants overexpressing CsBPCs and a Nicotiana benthamiana (tobacco) luciferase assay indicated that CsBPCs inhibited the expression of CsABI3. Transgenic plants overexpressing CsBPCs were constructed to confirm that CsBPCs participates in the control of seed germination. This study of the cucumber BPC-ABI3 pathway will help to explore and characterize the molecular mechanisms underlying seed germination and will provide necessary information for seed conservation in agriculture and forestry.

show abstract

“…While not necessarily affecting only PT growth, the Arabidopsis AtSUC1 and rice (Oryza sativa) OsSUT1 sucrose transporters have defective male gametophyte phenotypes (Sivitz et al, 2008;Hirose et al, 2010). In cucumber (Cucumis sativus), the hexose transporter CsHT1 is necessary for PT growth (Cheng et al, 2015). Despite probably being related to microsporogenesis and exine pattern formation, mutants of the PM-localized sucrose transporter AtRPG1/AtSWEET8 display fertility defects (Guan et al, 2008;Chen et al, 2010;Sun et al, 2013).…”

Section: Opposing Forces: Turgor and Cell Wall Depositionmentioning

confidence: 99%

Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes

et al. 2016

View full text Add to dashboard Cite

Down-Regulating CsHT1, a Cucumber Pollen-Specific Hexose Transporter, Inhibits Pollen Germination, Tube Growth, and Seed Development

Cited by 64 publications

References 60 publications

Cloning and functional characterization of quinolinic acid phosphoribosyl transferase (QPT) gene of Nicotiana tabacum

Cloning and functional characterization of quinolinic acid phosphoribosyl transferase (QPT) gene of Nicotiana tabacum

Cucumber CsBPCs Regulate the Expression of CsABI3 during Seed Germination

Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes

Contact Info

Product

Resources

About