Down-regulated non-coding RNA (lncRNA-ANCR) promotes osteogenic differentiation of periodontal ligament stem cells

Jia, Qiong; Jiang, Wenkai; Ni, Longxing

doi:10.1016/j.archoralbio.2014.10.007

Cited by 129 publications

(108 citation statements)

References 34 publications

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“…The induction medium was changed every 3 days. Finally, cells were stained with alizarin red (pH = 4.1) and staining quantified according to previously published methods55.…”

Section: Methodsmentioning

confidence: 99%

IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

Jiang

Luo

et al. 2017

Sci Rep

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During caries, dental pulp expresses a range of pro-inflammatory cytokines in response to the infectious challenge. Interferon gamma (IFN-γ) is a dimerized soluble cytokine, which is critical for immune responses. Previous study has demonstrated that IFN-γ at relative high concentration (100 ng/mL) treatment improved the impaired dentinogenic and immunosuppressive regulatory functions of disease-derived dental pulp stem cells (DPSCs). However, little is known about the regulatory effects of IFN-γ at relative low concentration on healthy DPSC behavior (including proliferation, migration, and multiple-potential differentiation). Here we demonstrate that IFN-γ at relatively low concentrations (0.5 ng/mL) promoted the proliferation and migration of DPSCs, but abrogated odonto/osteogenic differentiation. Additionally, we identified that NF-κB and MAPK signaling pathways are both involved in the process of IFN-γ-regulated odonto/osteogenic differentiation of DPSCs. DPSCs treated with IFN-γ and supplemented with pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) or SB203580 (a MAPK inhibitor) showed significantly improved potential for odonto/osteogenic differentiation of DPSCs both in vivo and in vitro. These data provide important insight into the regulatory effects of IFN-γ on the biological behavior of DPSCs and indicate a promising therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.

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“…The induction medium was changed every 3 days. Finally, cells were stained with alizarin red (pH = 4.1) and staining quantified according to previously published methods55.…”

Section: Methodsmentioning

confidence: 99%

IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

Jiang

Luo

et al. 2017

Sci Rep

Self Cite

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“…When the canonical WNT signaling pathway is suppressed, the osteogenic differentiation of PDLSC/ANCR-RNAi cells is inhibited too, indicating that Wnt/β-catenin signaling pathway may play a crucial role in the ANCR-mediated osteogenic differentiation of PDLSCs [ 86 ]. Nicotine and TNF-α can induce the osteogenic differentiation defi ciency of PDLSCs by activating WNT signaling [ 87 , 88 ], and down-regulation of β-catenin level can activate the non-canonical Wnt/Ca 2+ pathway, leading to the promotion of osteogenic differentiation in PDLSCs [ 89 ].…”

Section: Wnt/β-catenin Signaling Pathwaymentioning

confidence: 97%

Signaling Pathways in Dental Stem Cells During Their Maintenance and Differentiation

Liu

Zhou

et al. 2016

Stem Cell Biology and Regenerative Medicine

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“…Song et al identified 574 lncRNAs that were differentially expressed during osteogenic differentiation [23], and the function of some of these lncRNAs on osteogenic differentiation has been confirmed. For example, down-regulation of lncRNA-ANCR promotes osteogenic differentiation of periodontal ligament stem cells [11] and upregulation of lncRNA MEG3 promotes osteogenic differentiation of MSCs [23]. These studies indicate that lncRNAs play an important role in regulating osteogenic differentiation of MSCs.…”

Section: Discussionmentioning

confidence: 79%

“…Previous studies indicate that some lncRNAs play a role in regulating osteogenic differentiation of stem cells, such as AK141205, ANCR, and MEG3 [10][11][12]. Certain lncRNAs, such as ANCR, and H19, also promote osteoblast differentiation [13,14].…”

Section: Introductionmentioning

confidence: 99%

Silencing of Long Non-Coding RNA NONHSAT009968 Ameliorates the Staphylococcal Protein A-Inhibited Osteogenic Differentiation in Human Bone Mesenchymal Stem Cells

Cui

Lü

Tan

et al. 2016

Cell Physiol Biochem

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Background/Aims: Osteomyelitis is defined as an inflammation of the bones and bone marrow. The inflammatory microenvironment attenuates the osteogenic differentiation capacity of stem cells and inhibits osteoblast-mediated bone formation, leading to net bone loss. However, the whole expression profile, function and side effect of long non-coding RNAs (lncRNAs) on osteogenic differentiation of stem cells in an inflammatory microenvironment of osteomyelitis are not known. Methods: In the present study, human bone mesenchymal stem cells (hBMSCs) were treated with different concentrations of Staphylococcal protein A (SpA) to trigger an inflammatory microenvironment in vitro to partly duplicate the inflammatory microenvironment of osteomyelitis, which was confirmed using ELISA for detecting the inflammatory cytokines. The complete expression profiles of lncRNAs and mRNA during osteogenic differentiation of hBMSCs in an inflammatory microenvironment triggered by SpA were analyzed using a lncRNA microarray. LncRNA expression levels were verified by quantitative reverse transcription PCR analysis (qRT-PCR). The expression of NONHSAT009968 in hB-MSCs was silenced by infection with lentivirus expressing NONHSAT009968-shRNA. The expression of Runx2, OCN, OPN, COL1A1, and alkaline phosphatase (ALP) activity was detected by western blot. Alizarin red staining and ALP activity detection were carried out. Results: The results of ELISA showed that SpA treatment induced secretion of inflammatory cytokines IL-1A, IL-6, and TNFA. The results of alizarin red staining and ALP detection showed that SpA treatment suppressed the osteogenic differentiation of hBMSCs. A total of 2033 lncRNAs were found with aberrant expression in SpA-treated hBMSCs compared to controls. Among these lncRNAs, 641 were down-regulated and 1392 were up-regulated. Based on the results of qRT-PCR, lncRNA NONHSAT009968 was chosen for further investigation. The results of alizarin red staining, ALP activity detection, and western blot detection of Runx2, OCN, OPN, COL1A1, and ALP indicated that NONHSAT009968 silencing ameliorates SpA-inhibited osteogenic differentiation in hBMSCs. Conclusion: Our present study provides a basis for future analyses of the role of lncRNAs in osteoblastic differentiation in an inflammatory environment triggered by SpA, and lncRNA NONHSAT009968 might be a new target for promoting osteoblast formation.

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Down-regulated non-coding RNA (lncRNA-ANCR) promotes osteogenic differentiation of periodontal ligament stem cells

Cited by 129 publications

References 34 publications

IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

IFN-γ regulates human dental pulp stem cells behavior via NF-κB and MAPK signaling

Signaling Pathways in Dental Stem Cells During Their Maintenance and Differentiation

Silencing of Long Non-Coding RNA NONHSAT009968 Ameliorates the Staphylococcal Protein A-Inhibited Osteogenic Differentiation in Human Bone Mesenchymal Stem Cells

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