Down‐regulated miRs specifically correlate with non‐cardial gastric cancers and Lauren's classification system

Lorenzon, Laura; Cippitelli, Claudia; Avantifiori, Riccardo; Uccini, Stefania; French, Deborah; Torrisi, Maria Rosaria; Ranieri, Danilo; Mercantini, Paolo; Canu, Valeria; Blandino, Giovanni; Cavallini, Marco

doi:10.1002/jso.24648

Cited by 8 publications

(8 citation statements)

References 52 publications

(112 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…XIST has been reported to promote tumor cell apoptosis through a variety of apoptosis-related genes, such as Bcl-2, Bax, caspase 3 and caspase 9 [30,31,44,45]. Among these genes, only Bcl-2 has been reported to be a direct target of miR-204-5p [32]; therefore, we evaluated Bcl-2 expression. However, we have not yet determined whether other apoptosis-related genes are involved in this process.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Knockdown of the long noncoding RNA XIST suppresses glioma progression by upregulating miR-204-5p

Shen¹,

Xiong²,

Shao³

et al. 2020

J. Cancer

View full text Add to dashboard Cite

Background: Gliomas are the most prevalent primary malignant tumors of the central nervous system. Our previous study showed that miR-204-5p is a tumor suppressor gene in glioma. Bioinformatic analyses suggest that long noncoding RNA (lncRNA) X-inactive specific transcript (XIST) is a potential target gene of miR-204-5p. Methods: We analyzed the expression of XIST and miR-204-5p in glioma tissues and the correlation with glioma grade. A series of in vitro experiments were carried out to elucidate the role of XIST in glioma progression. A mouse xenograft model was established to detect whether knockdown of XIST can inhibit glioma growth. A luciferase assay was performed to determine whether XIST can bind to miR-204-5p and the binding specificity. Cells stably expressing shXIST or shNC were transfected with anti-miR-204-5p or anti-miR-204-5p-NC to evaluate whether XIST mediates the tumor-suppressive effects of miR-204-5p. Results: XIST was upregulated in glioma tissues compared with normal brain tissues (NBTs), while miR-204-5p expression was significantly decreased in glioma tissues compared with NBTs. Both XIST and miR-204-5p expression levels were clearly related to glioma grade, and the expression of XIST was obviously negatively correlated with miR-204-5p expression. Knockdown of XIST inhibited glioma cell proliferation, migration, and invasion, promoted apoptosis of glioma cells, inhibited tumor growth and increased the survival time in nude mice. miR-204-5p could directly bind to XIST and negatively regulate XIST expression. XIST mediated glioma progression by targeting miR-204-5p in glioma cells. XIST crosstalk with miR-204-5p regulated Bcl-2 expression to promote apoptosis. Conclusion: Our results provide evidence that XIST, miR-204-5p and Bcl-2 form a regulatory axis that controls glioma progression and can serve as a potential therapeutic target for glioma.

show abstract

Section: Discussionmentioning

confidence: 99%

“…2E). XIST has been demonstrated to mediate apoptosis by regulating Bcl-2 expression in both non-small cell lung cancer and cervical cancer [30,31], and Bcl-2 has also been confirmed to be a direct target of miR-204-5p [32]. Therefore, the expression of Bcl-2 was evaluated.…”

Section: Knockdown Of Xist Inhibited Proliferation and Promoted Apoptmentioning

confidence: 99%

Knockdown of the long noncoding RNA XIST suppresses glioma progression by upregulating miR-204-5p

Shen¹,

Xiong²,

Shao³

et al. 2020

J. Cancer

View full text Add to dashboard Cite

show abstract

“…Studies have long sought specific biological markers that could characterize GC [ 19 ]. However, no existing marker(s) have proven to be sufficiently specific to GC.…”

Section: Introductionmentioning

confidence: 99%

Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis

Liu

Zhang

et al. 2020

Cell Biosci

View full text Add to dashboard Cite

Background Gastric cancer (GC) is a malignancy with high morbidity/mortality, partly due to a lack of reliable biomarkers for early diagnosis. It is important to develop reliable biomarker(s) with specificity, sensitivity and convenience for early diagnosis. The role of tumour-associated macrophages (TAMs) and survival of GC patients are controversial. Macrophage colony stimulating factor (MCSF) regulates monocytes/macrophages. Elevated MCSF is correlated with invasion, metastasis and poor survival of tumour patients. IL-34, a ligand of the M-CSF receptor, acts as a “twin” to M-CSF, demonstrating overlapping and complimentary actions. IL-34 involvement in tumours is controversial, possibly due to the levels of M-CSF receptors. While the IL-34/M-CSF/M-CSFR axis is very important for regulating macrophage differentiation, the specific interplay between these cytokines, macrophages and tumour development is unclear. Methods A multi-factorial evaluation could provide more objective utility, particularly for either prediction and/or prognosis of gastric cancer. Precision medicine requires molecular diagnosis to determine the specifically mutant function of tumours, and is becoming popular in the treatment of malignancy. Therefore, elucidating specific molecular signalling pathways in specific cancers facilitates the success of a precision medicine approach. Gastric cancer tissue arrays were generated from stomach samples with TNM stage, invasion depth and the demography of these patients (n = 185). Using immunohistochemistry/histopathology, M-CSF, IL-34 and macrophages were determined. Results We found that IL-34 may serve as a predictive biomarker, but not as an independent, prognostic factor in GC; M-CSF inversely correlated with survival of GC in TNM III–IV subtypes. Increased CD68 + TAMs were a good prognostic factor in some cases and could be used as an independent prognostic factor in male T3 stage GC. Conclusion Our data support the potency of IL-34, M-CSF, TAMs and the combination of IL-34/TAMs as novel biological markers for GC, and may provide new insight for both diagnosis and cellular therapy of GC.

show abstract

“…Studies have long sought speci c biological markers that could characterize GC [19]. However, no existing marker(s) have proven to be su ciently speci c to GC.…”

mentioning

confidence: 99%

Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis

Liu

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background : Gastric cancer (GC) is a malignancy with high morbidity/mortality, partly due to a lack of reliable biomarkers for early diagnosis. It is important to develop reliable biomarker(s) with specificity, sensitivity and convenience for early diagnosis. The role of tumour-associated macrophages (TAMs) and survival of GC patients are controversial. Macrophage colony stimulating factor (MCSF) regulates monocytes/macrophages. Elevated MCSF is correlated with invasion, metastasis and poor survival of tumour patients. IL-34, a ligand of the MCSF receptor, acts as a “twin” to MCSF, demonstrating overlapping and complimentary actions. IL-34 involvement in tumours is controversial, possibly due to the levels of MCSF receptors. While the IL‑34/MCSF/MCSFR axis is very important for regulating macrophage differentiation, the specific interplay between these cytokines, macrophages and tumour development is unclear. Methods : A multi-factorial evaluation could provide more objective utility, particularly for either prediction and/or prognosis of gastric cancer. Precision medicine requires molecular diagnosis to determine the specifically mutant function of tumours, and is becoming popular in the treatment of malignancy. Therefore, elucidating specific molecular signalling pathways in specific cancers facilitates the success of a precision medicine approach. Gastric cancer tissue arrays were generated from stomach samples with TNM stage, invasion depth and the demography of these patients (n=185). Using immunohistochemistry/histopathology, MCSF, IL-34 and macrophages were determined. Results : We found that IL-34 may serve as a predictive biomarker, but not as an independent, prognostic factor in GC; MCSF inversely correlated with survival of GC in TNM III‑IV subtypes. Increased CD68 + TAMs were a good prognostic factor in some cases and could be used as an independent prognostic factor in male T3 stage GC. Conclusion : Our data support the potency of IL-34, MCSF, TAMs and the combination of IL‑34/TAMs as novel biological markers for GC, and may provide new insight for both diagnosis and cellular therapy of GC.

show abstract

Down‐regulated miRs specifically correlate with non‐cardial gastric cancers and Lauren's classification system

Cited by 8 publications

References 52 publications

Knockdown of the long noncoding RNA XIST suppresses glioma progression by upregulating miR-204-5p

Knockdown of the long noncoding RNA XIST suppresses glioma progression by upregulating miR-204-5p

Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis

Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis

Contact Info

Product

Resources

About