Down-regulated long non-coding RNA-ATB in preeclampsia and its effect on suppressing migration, proliferation, and tube formation of trophoblast cells

Liu, Xijing; Chen, Hongqin; Kong, Weiqi; Zhang, Yanping; Cao, Liyuan; Gao, Linbo; Zhou, Rong

doi:10.1016/j.placenta.2016.12.004

Cited by 39 publications

(31 citation statements)

References 13 publications

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“… 14 , 39 , 40 Moreover, other lncRNAs, including ATB , Uc.187, and RPAIN , have been reported to regulate trophoblast cell growth and/or invasion and/or apoptosis in PE. 17 , 18 , 41 Therefore, more potential PE-associated lncRNAs need to be identified whose functions require further exploration of their underlying biological mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE

Liu

et al. 2018

Molecular Therapy - Nucleic Acids

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The long noncoding RNA HOXA11-AS displays abnormal expression in numerous human diseases. However, its function and biological mechanisms remain unclear in preeclampsia (PE). In this study, we report that HOXA11-AS is significantly downregulated in preeclamptic placental tissues and could contribute to the occurrence and development of PE. Silencing of HOXA11-AS expression could significantly suppress trophoblast cell growth and migration, whereas HOXA11-AS overexpression facilitated cell growth in the HTR-8/SVneo, JEG3, and JAR cell lines. RNA-seq analysis also indicated that HOXA11-AS silencing preferentially regulated numerous genes associated with cell proliferation and cell migration. Mechanistic analyses showed that HOXA11-AS could recruit Ezh2 and Lsd1 protein and regulate RND3 mRNA expression in the nucleus. In the cytoplasm, HOXA11-AS modulates HOXA7 expression by sponged miR-15b-5p, affecting trophoblast cell proliferation. Together, these data confirm that aberrant expression of HOXA11-AS is involved in the occurrence and development of PE and may act as a prospective diagnosis and therapeutic target in PE.

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Section: Discussionmentioning

confidence: 99%

Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE

Liu

et al. 2018

Molecular Therapy - Nucleic Acids

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“…The lncRNA taurine upregulated 1 (TUG1) was found to be markedly downregulated in placental samples from patients with PE, and TUG1 modulated trophoblast cell proliferation via epigenetic suppression of Rho family GTPase 3 . In addition, the lncRNA‐ATB was demonstrated to be involved in the pathogenesis of PE by regulating the process of trophoblast invasion and endovascular formation . The newly identified lncRNA, small nucleolar RNA host gene 5 (SNHG5), was revealed as an important lncRNA in the pathogenesis and progression of different types of cancers including bladder cancer, chronic myeloid leukemia, colorectal cancer, and gastric cancer .…”

Section: Introductionmentioning

confidence: 99%

The lncRNA small nucleolar RNA host gene 5 regulates trophoblast cell proliferation, invasion, and migration via modulating miR‐26a‐5p/N‐cadherin axis

Yang

Lan

et al. 2018

J of Cellular Biochemistry

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Pre-eclampsia (PE) is a pregnancy-specific disease characterized by the occurrence of hypertension and proteinuria after two weeks of gestation. Long noncoding RNAs (lncRNAs) are emerging as key regulators in PE development.This study aims to investigate the role of lncRNA, small nucleolar RNA host gene 5 (SNHG5), in the pathogenesis of PE. The expression of SNHG5 was significantly downregulated in placental tissues from patients with severe PE compared normal controls. Overexpression of SNHG5 promoted trophoblast (HTR-8/SVneo) cell proliferation, invasion, and migration, and flow cytometry results showed that SNHG5 overexpression inhibited apoptosis and caused a decrease of cell population at the G 0 /G 1 phase and an increase of cell population at the S phase, while knockdown of SNHG5 had the opposite effects. The interaction between SNHG5 and miR-26a-5p was predicted by bioinformatics analysis and confirmed by luciferase reporter assay and RNA immunoprecipitation, and miR-26a-5p was negatively regulated by SNHG5; miR-26a-5p expression was upregulated in PE placental tissues and was inversely correlated with SNHG5 expression. Furthermore, miR-26a-5p was predicted to target the 3′ untranslated region of N-cadherin, which was confirmed by luciferase reporter assay, and miR-26a-5p overexpression suppressed N-cadherin expression in HTR-8/SVneo cells. N-cadherin mRNA expression was downregulated in PE placental tissues and was positively correlated with SNHG5 expression.Both overexpression of miR-26a-5p and knockdown of N-cadherin suppressed HTR-8/SVneo cell invasion and migration, and also attenuated the effects of SNHG5 on the cellular functions of HTR-8/SVneo cells. In conclusion, our study suggested that SNHG5 promotes trophoblast cell proliferation, invasion, and migration at least partly via regulating the miR-26a-5p/N-cadherin axis. K E Y W O R D Sinvasion and migration, miR-26a-5p, N-cadherin, pre-eclampsia (PE), small nucleolar RNA host gene 5 (SNHG5), trophoblast J Cell Biochem. 2019;120:3173-3184.wileyonlinelibrary.com/journal/jcb

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“…Our previous study also demonstrated that lncRNA SPRY4-IT1 and MEG3 modulate trophoblast cell proliferation, apoptosis as well as tube formation, invasion and migration in this disorder [14,40,41]. Moreover, other lncRNAs, including ATB, Uc.187, RPAIN and so on, were reported to regulate trophoblast cell growth or/and invasion or/and apoptosis in PE [17,18,42]. Therefore, more and more potential PE-associated lncRNAs need to be identified, the functions of which require further exploration for their underlying biological mechanisms.…”

Section: Discussionmentioning

confidence: 79%

Down-regulated Long Noncoding RNAHOXA11-ASaffects trophoblast cell proliferation and migration by regulatingRND3andHOXA7expression in preeclampsia

Sun

et al. 2018

Preprint

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The long noncoding RNA HOXA11-AS reveals abnormal expression in numerous human diseases. However, its function and biological mechanisms remain unclear in Preeclampsia (PE). In this study, we report that HOXA11-AS was significantly downregulated in preeclampsic placental tissues and could contribute to the occurrence and development of Preeclampsia. Silencing of HOXA11-AS expression could significantly suppress trophoblast cell growth and migration, whereas HOXA11-AS overexpression facilitated cell growth in HTR-8/SVneo, JEG3 and JAR cell lines. RNA-seq analysis also indicated that HOXA11-AS silencing preferentially regulated numerous genes associated with cell proliferation and cell migration. Mechanistic analyses showed that HOXA11-AS could recruit Ezh2 and Lsd1 protein, and regulate RND3 mRNA expression in nucleus. In cytoplasm, HOXA11-AS modulate HOXA7 expression by sponged miR-15b-5p, thus affecting trophoblast cell proliferation. Together, these resulting data confirm that aberrant expression of HOXA11-AS is involved in the occurrence and development of Preeclampsia, and may act as a prospective diagnosis and therapeutic target in PE.

show abstract

Down-regulated long non-coding RNA-ATB in preeclampsia and its effect on suppressing migration, proliferation, and tube formation of trophoblast cells

Cited by 39 publications

References 13 publications

Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE

Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE

The lncRNA small nucleolar RNA host gene 5 regulates trophoblast cell proliferation, invasion, and migration via modulating miR‐26a‐5p/N‐cadherin axis

Down-regulated Long Noncoding RNAHOXA11-ASaffects trophoblast cell proliferation and migration by regulatingRND3andHOXA7expression in preeclampsia

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