Doublecortin Is a Highly Valuable Endogenous Marker of Adult Neurogenesis in Canaries

Balthazart, Jacques; Ball, Gregory F.

doi:10.1159/000362917

Cited by 30 publications

(27 citation statements)

References 16 publications

(30 reference statements)

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“…Since changes in neurogenesis are reflected in the number of neuronal precursors, DCX has been widely used to analyze modulation in the levels of neurogenesis . Presently, mice were sacrificed by CO 2 , brains removed and frozen brain tissue sections (20 μm) prepared using MEV Slee Semi‐Automatic Cryostat (SLEE medical GmbH, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Since changes in neurogenesis are reflected in the number of neuronal precursors, DCX has been widely used to analyze modulation in the levels of neurogenesis [31,32]. Presently, mice were sacrificed by CO 2 , brains removed and frozen brain tissue sections (20 μm) prepared using MEV Slee Semi-Automatic Cryostat (SLEE medical GmbH, Germany It should be noted that although the head is a complex structure containing many tissue layers, in mice these layers are very thin (skull~500 μm, cortical thickness~1 mm, brain <10 mm) and therefore we consider the head as a homogenous medium.…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Optically derived metabolic and hemodynamic parameters predict hippocampal neurogenesis in the BTBR mouse model of autism

Abookasis

Lerman

Roth

et al. 2017

Journal of Biophotonics

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In this study, we made use of dual-wavelength laser speckle imaging (DW-LSI) to assess cerebral blood flow (CBF) in the BTBR-genetic mouse model of autism spectrum disorder, as well as control (C57Bl/6J) mice. Since the deficits in social behavior demonstrated by BTBR mice are attributed to changes in neural tissue structure and function, we postulated that these changes can be detected optically using DW-LSI. BTBR mice demonstrated reductions in both CBF and cerebral oxygen metabolism (CMRO ), as suggested by studies using conventional neuroimaging technologies to reflect impaired neuronal activation and cognitive function. To validate the monitoring of CBF by DW-LSI, measurements with laser Doppler flowmetry (LDF) were also performed which confirmed the lowered CBF in the autistic-like group. Furthermore, we found in vivo cortical CBF measurements to predict the rate of hippocampal neurogenesis, measured ex vivo by the number of neurons expressing doublecortin or the cellular proliferation marker Ki-67 in the dentate gyrus, with a strong positive correlation between CBF and neurogenesis markers (Pearson, r = 0.78; 0.9, respectively). These novel findings identifying cortical CBF as a predictive parameter of hippocampal neurogenesis highlight the power and flexibility of the DW-LSI and LDF setups for studying neurogenesis trends under normal and pathological conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Optically derived metabolic and hemodynamic parameters predict hippocampal neurogenesis in the BTBR mouse model of autism

Abookasis

Lerman

Roth

et al. 2017

Journal of Biophotonics

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“…Although Balthazart and Ball [2014] acknowledge that DCX can be expressed by mature neurons, it is unclear why they believe that including these adult DCX-expressing neurons in their quantifications of young migrating neurons has no impact on the outcome of their quantifications. The suggestion that only very few mature neurons express DCX is misleading, and requires verification for each brain area under investigation.…”

Section: Expression In Mature Neuronsmentioning

confidence: 99%

“…BrdU-labeling typifies the age of a cell, but gives no specific information about the cell's functional state. Balthazart and Ball [2014] correctly address the fact that the functional state of a neuron of a certain age can differ in different parts of the brain, potentially affecting the relationship between BrdUand DCX-labeling. Whether the large regional differences that we observed can be explained by the methodological differences between BrdU-and DCX-labelAntibodies are one of the most frequently used research tools in the comparative neurosciences.…”

mentioning

confidence: 97%

“…The suggestion of Balthazart and Ball [2014] that these DCX-expressing mid-and hindbrain regions may constitute 'undiscovered' neurogenic brain areas seems unlikely, and over 30 years of neurogenesis research in the canary brain do not support it. It is possible to detect small numbers of BrdU-labeled cells in the songbird mid-and hindbrain after BrdU injection, but we have demonstrated before that these BrdU-labeled cells are not associated with neuron recruitment and are not immunoreactive for DCX or other neuronal markers [Vellema et al, 2010[Vellema et al, , 2014.…”

mentioning

confidence: 99%

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What Makes a Marker a Good Marker?

Vellema¹,

Ko²,

Frankl-Vilches³

et al. 2014

Brain Behav Evol

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Potential Adult Neurogenesis in the Telencephalon and Cerebellar Cortex of the Nile Crocodile Revealed with Doublecortin Immunohistochemistry

Ngwenya

Patzke

Herculano‐Houzel

et al. 2017

The Anatomical Record

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The brain of the crocodile is known to gain in mass allometrically throughout life, and the addition of neurons (as well as non-neurons) appears to play a significant role in this increasing brain mass. We used immunohistochemistry in the brains of 12 Nile crocodiles ranging between 350 g and 86 kg in body mass and 1.99 g to 7.9 g in brain mass to identify the regions of the brain in which neurons immunopositive for doublecortin (DCX), a marker for potential adult neurogenesis, are found. Similar to other reptiles, potential newly born neurons, those immunopositive for DCX, were found throughout the telencephalon, the main and accessory olfactory bulbs and the olfactory tract, and in the cerebellar cortex; however, no DCX immunopositive neurons were observed in the diencephalon or brainstem. An apparent moderate decrease in the density of DCX labeled neurons in the olfactory bulbs and tract as well as the cerebellar cortex was observed with increasing brain mass, but the observed qualitative density of labeled neurons within the telencephalon was maintained irrespective of brain mass. Three potential neurogenic zones, within the sulci of the lateral ventricle, were identified, and these are similar to those seen in other reptiles. This study indicates that at least part of the gain in brain mass with age in the Nile crocodile may be accounted for by the potential addition and integration of new neurons into the existing circuitry, especially so for the olfactory system, telencephalon and cerebellar cortex. Anat Rec, 301:659-672, 2018. © 2017 Wiley Periodicals, Inc.

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Doublecortin Is a Highly Valuable Endogenous Marker of Adult Neurogenesis in Canaries

Cited by 30 publications

References 16 publications

Optically derived metabolic and hemodynamic parameters predict hippocampal neurogenesis in the BTBR mouse model of autism

Optically derived metabolic and hemodynamic parameters predict hippocampal neurogenesis in the BTBR mouse model of autism

What Makes a Marker a Good Marker?

Potential Adult Neurogenesis in the Telencephalon and Cerebellar Cortex of the Nile Crocodile Revealed with Doublecortin Immunohistochemistry

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