Double thermoresponsive polybetaine-based ABA triblock copolymers with capability to condense DNA

Dai, Fengying; Wang, Pengfei; Wang, Ying; Tang, Lei; Yang, Jianhai; Liu, Wenguang; Li, Hexian; Wang, Guochang

doi:10.1016/j.polymer.2008.09.060

Cited by 46 publications

(41 citation statements)

References 40 publications

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“…Poly(sulfobetaine methacrylate) (PSBMA) has proven promising in the hydrophilic modification of polycation gene vectors because of the biocompatibility, which provides a fouling resistance to plasma proteins due to the surface hydrophilicity resulting from the charge bias . PDMAEMA‐b‐PSBMA has been observed to exhibit lower cytotoxicity than homo‐PDMAEMA, while retaining a good DNA condensation capability and achieving much superior gene transfection efficiency . Furthermore, PDMAEMA‐b‐PSBMA could be grafted onto luminescent carbon dots for facile gene transfection detection and imaging .…”

Section: Introductionmentioning

confidence: 99%

Microscopic insight into the DNA condensation process of a zwitterion‐functionalized polycation

et al. 2016

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Zwitterion-functionalized polycations are ideal gene carriers with long circulation, high cellular uptaking and low cell viability. However, the trade-off between the DNA condensation efficiency and the cell viability must be addressed. The purpose of this study is to provide a microscopic insight into the DNA condensation process and to explore the effect of a zwitterionic block of zwitterion-functionalized polycation, which is of great significance in designing novel gene delivery systems. Poly[2-(dimethylamino)ethyl methacrylate-b-(sulfobetaine methacrylate)] (PDMAEMA-b-PSBMA) copolymers were synthesized and used as the model systems. Different from the conventional concept that the PSBMA zwitterionic block act only as the "stealthy" groups, the subtle differences in physical and colloidal characteristics between the polycation/DNA polyplexes show that the PSBMA segment is capable of wrapping DNA attributed to the quaternary ammonium cations, without compromising the DNA condensation capability. On the other hand, the incorporation of PSBMA block reduces the surface charge of the polyplexes, which substantially result in the inefficient transfection and the reduced cytotoxicity.

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Section: Introductionmentioning

confidence: 99%

Microscopic insight into the DNA condensation process of a zwitterion‐functionalized polycation

et al. 2016

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“…The polymer brushes were observed to be highly extended at pH = 2 and above pH= 9, when the structure became positively and negatively charged, respectively [117]. In the pH range of 2-9.5, the polymer brushes were in a polyzwitterionic state as poly (83). Polymer brushes Poly (83) showed antifouling properties and low cytotoxicity to human dermal fibroblast cells for brushes having a thickness of 24-28 nm.…”

Section: Reversible Double Switchmentioning

confidence: 99%

“…UCST can be further modulated by the copolymerisation of monomer (38) with other monomers containing hydrophobic pentyl-(PAA), benzyl-(BAA) and dodecylacrylamide (DAA) functionalities [81], N-vinyl caprolactame (VCL) [82] or blocks of 2-(2-methoxyethoxy)ethyl methacrylate (PEG MA) [83], N-isopropyl acrylamide (NIPAM) [84,85] (Figure 6). Hydrophobic monomers in copolymers typically provide an increase in UCST and the presence of a simple electrolyte decreases UCST, which can easily modulate the solubility properties of polymers [81].…”

Section: Physically Induced Reversible Switch In Solutionsmentioning

confidence: 99%

“…In the pH range of 2-9.5, the polymer brushes were in a polyzwitterionic state as poly (83). Polymer brushes Poly (83) showed antifouling properties and low cytotoxicity to human dermal fibroblast cells for brushes having a thickness of 24-28 nm. Poly(83) exhibited good long-term stability under physiological condition, while the hydrolysis at pH < 8 was observed [117].…”

Section: Reversible Double Switchmentioning

confidence: 99%

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Switchable Materials Containing Polyzwitterion Moieties

2015

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Abstract:In recent decades, the design and construction of smart materials capable of switching into a polyzwitterionic state by an external trigger have been intensively pursued. Polyzwitterionic states have unique antifouling and surface properties and external triggers, such as pH, light, ions, electric field and CO 2 , cause significant changes in materials with regard to overall charge, ionic strength and wettability. This survey highlights current progress in the irreversible as well as the reversible switching process involving polyzwitterionic moieties, which can, in turn, be applied to studying the interaction of various interfaces with biological species as protein, DNA, bacteria or platelets and also for advanced use.

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“…Although much work has been conducted on the interactions between DNA and amphiphilic copolymers [12][13][14] or cationic polymers [4,5], detailed characterization of the interactions between DNA and fluorine-containing amphiphilic cationic copolymers is lacked. Fluorine-containing copolymers have been studied from the perspective of biomedical applications, for instance, blood substitutes, gas carriers, bioconversion, extraction, and so forth, which cannot be achieved by non-fluorinated copolymers.…”

Section: Introductionmentioning

confidence: 99%

Study on the interaction between a fluorine-containing amphiphilic cationic copolymer and nucleic acid by resonance light scattering technique

Pan

Xiong

et al. 2011

Journal of Fluorine Chemistry

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Double thermoresponsive polybetaine-based ABA triblock copolymers with capability to condense DNA

Cited by 46 publications

References 40 publications

Microscopic insight into the DNA condensation process of a zwitterion‐functionalized polycation

Microscopic insight into the DNA condensation process of a zwitterion‐functionalized polycation

Switchable Materials Containing Polyzwitterion Moieties

Study on the interaction between a fluorine-containing amphiphilic cationic copolymer and nucleic acid by resonance light scattering technique

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