Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates

Fraga, Jorge; Rojas, Lázara; Sariego, Idalia; Fernández-Calienes, Aymé

doi:10.1590/s1413-86702005000600012

Cited by 19 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high prevalence (43.5%) of TVVs found in this study is comparable to a prevalence of 55% (95% CI: 38.4–70.7) among Cuban isolates [ 36 ]. Two recent studies have reported lower carriage of TVV, 18.7% (95% CI: 11.5–28.0) in the Philippines [ 37 ] and 17.3% (95% CI: 7.8–31.4) among Iranian isolates [ 38 ], although the latter study only determined the presence of TVV1.…”

Section: Discussionsupporting

confidence: 65%

Molecular typing of Trichomonas vaginalis isolates by actin gene sequence analysis and carriage of T. vaginalis viruses

et al. 2017

View full text Add to dashboard Cite

BackgroundThe protozoan parasite Trichomonas vaginalis is the most common non-viral, sexually transmitted pathogen. Although T. vaginalis is highly prevalent among women in Kenya, there is lack of data regarding genetic diversity of isolates currently in circulation in Kenya.MethodsTyping was performed on 22 clinical isolates of T. vaginalis collected from women attending the antenatal care clinic at Kilifi County Hospital, Kenya, in 2015. Genotyping followed a previously proposed restriction fragment length polymorphism (RFLP) scheme, which involved in silico cleavage of the amplified actin gene by HindII, MseI and RsaI restriction enzymes. Phylogenetic analysis of all the sequences was performed to confirm the results obtained by RFLP-analysis and to assess the diversity within the RFLP genotypes. Additionally, we determined carriage of the four different types of Trichomonas vaginalis viruses (TVVs) by polymerase chain reaction.ResultsIn silico RFLP-analysis revealed five actin genotypes; 50.0% of the isolates were of actin genotype E, 27.3% of actin genotype N, 13.6% of actin genotype G and 4.5% of actin genotypes I and P. Phylogenetic analysis was in agreement with the RFLP-analysis, with the different actin genotypes clustering together. Prevalence of TVVs was 43.5% (95% confidence interval, CI: 23.2–65.5). TVV1 was the most prevalent, present in 39.1% of the strains and 90% of the T. vaginalis isolates which harbored TVVs had more than one type of TVV. None of the isolates of actin genotype E harbored any TVV.ConclusionThe presence of five actin genotypes in our study suggests notable diversity among T. vaginalis isolates occurring among pregnant women in Kilifi, Kenya. Isolates of the most prevalent actin genotype E lacked TVVs. We found no association between T. vaginalis genotype, carriage of TVVs and symptoms. Further studies with higher number of strains should be conducted in order to corroborate these results.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2496-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 65%

Molecular typing of Trichomonas vaginalis isolates by actin gene sequence analysis and carriage of T. vaginalis viruses

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Nevertheless, in a study of adolescents with T. vaginalis infection, clinical symptoms e.g. vaginal discharge, dysuria, dyspareunia, and cervical erythema were significantly associated with TVV-positive status of the vaginal TV isolates, detectable by nucleic acids gel electrophoresis [50]. More efficient and cost-effective diagnostics and surrogate biomarkers are urgently needed for further clinical observational and interventional studies.…”

Section: Resultsmentioning

confidence: 99%

Endobiont Viruses Sensed by the Human Host – Beyond Conventional Antiparasitic Therapy

et al. 2012

View full text Add to dashboard Cite

Wide-spread protozoan parasites carry endosymbiotic dsRNA viruses with uncharted implications to the human host. Among them, Trichomonas vaginalis, a parasite adapted to the human genitourinary tract, infects globally ∼250 million each year rendering them more susceptible to devastating pregnancy complications (especially preterm birth), HIV infection and HPV-related cancer. While first-line antibiotic treatment (metronidazole) commonly kills the protozoan pathogen, it fails to improve reproductive outcome. We show that endosymbiotic Trichomonasvirus, highly prevalent in T. vaginalis clinical isolates, is sensed by the human epithelial cells via Toll-like receptor 3, triggering Interferon Regulating Factor -3, interferon type I and proinflammatory cascades previously implicated in preterm birth and HIV-1 susceptibility. Metronidazole treatment amplified these proinflammatory responses. Thus, a new paradigm targeting the protozoan viruses along with the protozoan host may prevent trichomoniasis-attributable inflammatory sequelae.

show abstract

“…A few case studies comparing different aspects of trichomoniasis with the presence of TVV in T . vaginalis clinical isolates have noted correlations with patient signs and symptoms, but the case numbers have remained small (5, 13, 20). …”

Section: Introductionmentioning

confidence: 99%

Structure of a Protozoan Virus from the Human Genitourinary Parasite Trichomonas vaginalis

Parent

Takagi

Cardone

et al. 2013

mBio

View full text Add to dashboard Cite

The flagellated protozoan Trichomonas vaginalis is an obligate human genitourinary parasite and the most frequent cause of sexually transmitted disease worldwide. Most clinical isolates of T. vaginalis are persistently infected with one or more double-stranded RNA (dsRNA) viruses from the genus Trichomonasvirus, family Totiviridae, which appear to influence not only protozoan biology but also human disease. Here we describe the three-dimensional structure of Trichomonas vaginalis virus 1 (TVV1) virions, as determined by electron cryomicroscopy and icosahedral image reconstruction. The structure reveals a T = 1 capsid comprising 120 subunits, 60 in each of two nonequivalent positions, designated A and B, as previously observed for fungal Totiviridae family members. The putative protomer is identified as an asymmetric AB dimer consistent with either decamer or tetramer assembly intermediates. The capsid surface is notable for raised plateaus around the icosahedral 5-fold axes, with canyons connecting the 2- and 3-fold axes. Capsid-spanning channels at the 5-fold axes are unusually wide and may facilitate release of the viral genome, promoting dsRNA-dependent immunoinflammatory responses, as recently shown upon the exposure of human cervicovaginal epithelial cells to either TVV-infected T. vaginalis or purified TVV1 virions. Despite extensive sequence divergence, conservative features of the capsid reveal a helix-rich fold probably derived from an ancestor shared with fungal Totiviridae family members. Also notable are mass spectrometry results assessing the virion proteins as a complement to structure determination, which suggest that translation of the TVV1 RNA-dependent RNA polymerase in fusion with its capsid protein involves −2, and not +1, ribosomal frameshifting, an uncommonly found mechanism to date.

show abstract

Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates

Cited by 19 publications

References 19 publications

Molecular typing of Trichomonas vaginalis isolates by actin gene sequence analysis and carriage of T. vaginalis viruses

Molecular typing of Trichomonas vaginalis isolates by actin gene sequence analysis and carriage of T. vaginalis viruses

Endobiont Viruses Sensed by the Human Host – Beyond Conventional Antiparasitic Therapy

Structure of a Protozoan Virus from the Human Genitourinary Parasite Trichomonas vaginalis

Contact Info

Product

Resources

About