Double-stranded RNA targeting vATPase B reveals a potential target for pest management of Henosepilachna vigintioctopunctata

Lü, Jing; Guo, Mujuan; Chen, Shimin; Noland, Jeffrey Edward; Guo, Wei; Sang, Wen; Qi, Yixin; Qiu, Bao‐Li; Zhang, Youjun; Yang, Chunxiao; Pan, Huipeng

doi:10.1016/j.pestbp.2020.104555

Cited by 22 publications

(21 citation statements)

References 48 publications

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“… Lü et al (2020a , b , 2021a , b) and Guo et al (2021) examined RNAi efficacy via oral delivery of dsRNAs targeting various genes in the 28-spotted potato ladybird ( H. vigintioctopunctata ). Lü et al (2020b ) observed approximately 60% mortality (8 days, target gene Snf7 ) and 70–85% mortality (10 days, targeting eight different coatomer components) via feeding on leaves treated with dsRNA at a concentration of 0.005 μg dsRNA/μl.…”

Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

“… Guo et al (2021) observed nearly complete mortality after 7 days (target gene vATPase E ) of feeding on leaves treated with dsRNA at a concentration of 0.01 μg dsRNA/μl. These studies by Lü et al (2020a , b , 2021a , b) and Guo et al (2021) additionally examined dietary RNAi efficacy in H. vigintioctopunctata first- and third-instar larvae and adults, using bacterially expressed dsRNA and demonstrated higher and faster RNAi efficacy in larvae compared to adults. Finally, Ren et al (2021) observed significant growth retardation in H. vigintioctopunctata via consumption of transgenic potato plants.…”

Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

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Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Willow

Veromann

2021

Front. Plant Sci.

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Many herbivorous beetles (Order Coleoptera) contribute to serious losses in crop yields and forest trees, and plant biotechnology solutions are being developed with the hope of limiting these losses. Due to the unprecedented target-specificity of double-stranded RNA (dsRNA), and its utility in inducing RNA interference (RNAi) when consumed by target pest species, dsRNA-based plant biotechnology approaches represent the cutting edge of current pesticide research and development. We review dietary RNAi studies in coleopterans and discuss prospects and future directions regarding RNAi-based management of coleopteran plant pests. Herein, we also provide a balanced overview of existing studies in order to provide an accurate re-assessment of dietary RNAi sensitivity in coleopterans, despite the limitations to the existing body of scientific literature. We further discuss impediments to our understanding of RNAi sensitivity in this important insect order and identify critical future directions for research in this area, with an emphasis on using plant biotechnology approaches.

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Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

Section: Observed Rnai Sensitivities In Coleopteramentioning

confidence: 99%

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Willow

Veromann

2021

Front. Plant Sci.

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“…The success of RNAi-based strategies in future pest management will rely on the identification of essential target genes that confer lethal phenotypic effects upon knockdown [ 28 ]. Generally, functional genes involved in insect development, metamorphosis, or key metabolic processes may be suitable RNAi targets as part of control strategies of insects [ 15 , 16 , 17 , 18 , 29 , 30 , 31 , 32 , 33 ]. Previous studies have shown that trehalase plays pivotal roles in various physiological processes, including energy metabolism [ 34 ], chitin synthesis during molting [ 35 ], and diapause [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…RNA interference (RNAi) technology is a powerful tool for functional studies that can be used to selectively suppress the expression of target genes both in vitro and in vivo [ 13 , 14 , 15 , 16 , 17 , 18 ]. RNA interference has been widely explored as an alternative to conventional management methods for controlling insect pests [ 15 , 16 , 17 , 18 ]. For example, bacterially expressed double-stranded RNA (dsRNA) from the lesswright ( lwr ) gene of Henosepilachna vigintioctopunctata was applied to eggplant leaves and caused 88% mortality for the 1st larvae of H. vigintioctopunctata , 66% mortality for the 3rd instar larvae, and 36% mortality to the adults after 10 d, 10 d, and 14 d of application, respectively [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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De Novo Assembly of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) Transcriptome across Developmental Stages

Yang

Guo

et al. 2020

IJMS

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Asian citrus psyllid Diaphorina citri Kuwayama is an important economic pest of citrus, as it transmits Candidatus Liberibacter asiaticus, the causative agent of huanglongbing. In this study, we used RNA-seq to identify novel genes and provide the first high-resolution view of the of D. citri transcriptome throughout development. The transcriptomes of D. citri during eight developmental stages, including the egg, five instars, and male and female adults were sequenced. In total, 115 million clean reads were obtained and assembled into 354,726 unigenes with an average length of 925.65 bp and an N50 length of 1733 bp. Clusters of Orthologous Groups, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to functionally annotate the genes. Differential expression analysis highlighted developmental stage-specific expression patterns. Furthermore, two trehalase genes were characterized with lower expression in adults compared to that in the other stages. The RNA interference (RNAi)-mediated suppression of the two trehalase genes resulted in significantly high D. citri mortality. This study enriched the genomic information regarding D. citri. Importantly, these data represent the most comprehensive transcriptomic resource currently available for D. citri and will facilitate functional genomics studies of this notorious pest.

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Efficacy of RNA interference using nanocarrier‐based transdermal dsRNA delivery system in the woolly apple aphid, Eriosoma lanigerum

Guo

Fan

Teng

et al. 2022

Arch Insect Biochem Physiol

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RNA interference (RNAi) is an essential approach for studying gene function and has been considered as a promising strategy for pest control. However, RNAi method has not been conducted in Woolly apple aphid (Eriosoma lanigerum Hausmann), one of the most damaging apple pests in the world. In the study, we investigated the efficacy of RNAi of V-ATPase subunit D (ATPD), an efficacious target for RNAi in other insects, in E. lanigerum by a transdermal double-stranded RNA (dsRNA) delivery system with nanocarriers. Our results showed although topical application of dsATPD in E. lanigerum for 24 h produced 40.5% gene silencing, the additional help of nanocarriers extremely improved the interference efficiency with 98.5% gene silencing. Moreover, a 55.75% mortality was observed 5 days after topical application of nanocarriers and dsATPD, relative to the control (topical application of nanocarriers and double-stranded green fluorescent protein [dsGFP]). The nanocarrier-based transdermal dsRNA delivery system will promote the development of functional analysis of vital genes and also provide a potential target for RNAi-based management of E. lanigerum.

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Double-stranded RNA targeting vATPase B reveals a potential target for pest management of Henosepilachna vigintioctopunctata

Cited by 22 publications

References 48 publications

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

Highly Variable Dietary RNAi Sensitivity Among Coleoptera

De Novo Assembly of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) Transcriptome across Developmental Stages

Efficacy of RNA interference using nanocarrier‐based transdermal dsRNA delivery system in the woolly apple aphid, Eriosoma lanigerum

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