Double-stranded RNA reduces growth rates of the gut parasite Crithidia mellificae

Pereira, Kleber de Sousa; Piot, Niels; Smagghe, Guy; Meeus, Ivan

doi:10.1007/s00436-018-6176-0

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…nidulans and other filamentous fungi (Cornman et al, 2012;Dang et al, 2011) ;and iv) introduce dsRNA to the spores as-is because we presumed that germinating spores will uptake or bind to exogenous genetic material upon CO 2 activation and without the need for transfection reagents, as observed in other fungi and microorganisms (Calkins et al, 2018;de Sousa Pereira, Piot, Smagghe, & Meeus, 2019;Jöchl et al, 2009) . To this end we searched the most recent As.…”

Section: Cloning and Dsrna Productionmentioning

confidence: 98%

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Tauber

Einspanier

Evans

et al. 2019

Preprint

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AbstractThere are viral, fungal, bacterial and trypanosomal pathogens that negatively impact the individual and superorganismal health of the western honey bee. One fungal pathogen, Ascosphaera apis, affects larvae and causes the disease chalkbrood. A previous genome analysis of As. apis revealed that its genome encodes for RNA interference genes, similar to other fungi and eukaryotes. Here, we examined whether As. apis-targeting double-stranded RNA species could disrupt the germination of As. apis. We observed that when spores were co-incubated with As. apis-targeting dsRNA, fewer spores were activated for germination, suggesting an uptake of exogenous genetic material at the very onset of germination and consequent damage to essential transcripts needed for germination. Overall, these results indicate that the causative agent of chalkbrood disease, As. apis, can be successfully targeted using an RNAi-based strategy.

show abstract

Section: Cloning and Dsrna Productionmentioning

confidence: 98%

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Tauber

Einspanier

Evans

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

RNA Interference to Modify Phenotypes in Agriculturally Important Pest and Beneficial Insects: Useful Examples and Future Challenges

Schutter¹,

Smagghe²

2022

Transgenic Insects

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Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Tauber

Einspanier

Evans

et al. 2020

Journal of Apicultural Research

View full text Add to dashboard Cite

There are viral, fungal, bacterial and trypanosomal pathogens that negatively impact the individual and superorganismal health of the western honey bee. One fungal pathogen, Ascosphaera apis , affects larvae and causes the disease chalkbrood. A previous genome analysis of As. apis revealed that its genome encodes for RNA interference genes, similar to other fungi and eukaryotes. Here, we examined whether As. apis -targeting double-stranded RNA species could disrupt the germination of As. apis .We observed that when spores were co-incubated with As. apis -targeting dsRNA, fewer spores were activated for germination, suggesting an uptake of exogenous genetic material at the very onset of germination and consequent damage to essential transcripts needed for germination. Overall, these results indicate that the causative agent of chalkbrood disease, As. apis , can be successfully targeted using an RNAi-based strategy. also made available for use under a CC0 license.

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Double-stranded RNA reduces growth rates of the gut parasite Crithidia mellificae

Cited by 3 publications

References 38 publications

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

RNA Interference to Modify Phenotypes in Agriculturally Important Pest and Beneficial Insects: Useful Examples and Future Challenges

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

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