Double-stranded RNA injected into female Black Tiger shrimp (Penaeus monodon) prior to spawning does not transfer to progeny

Rao, Min; Cowley, Jeff A.; Murphy, Brian; Stratford, Chris; Sellars, Melony J.

doi:10.1016/j.aquaculture.2018.09.059

Cited by 3 publications

(3 citation statements)

References 46 publications

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“…Female shrimp injected with dsRNA antivirals spawned progenies without any detectable amounts of these dsRNA; however, the injected dsRNA were distributed in different organs, including the ovaries. These dsRNAs were not detected in any of the larval (eggs, nauplii, mysis, and protozoea) or postlarval life stages . Further research should be conducted regarding whether these dsRNAs cause genetic modifications (e.g., epigenetics).…”

Section: Safety Of Rnai Usagementioning

confidence: 97%

“…These dsRNAs were not detected in any of the larval (eggs, nauplii, mysis, and protozoea) or postlarval life stages. 137 Further research should be conducted regarding whether these dsRNAs cause genetic modifications (e.g., epigenetics). Meanwhile it appears, until now, that the use of RNAi in aquatic organisms did not have any negative effects and that there is a low possibility of environmental risks that could arise from an escaped exotic population.…”

Section: ■ Safety Of Rnai Usagementioning

confidence: 99%

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RNA Interference in Aquaculture: A Small Tool for Big Potential

Abo‐Al‐Ela

2021

J. Agric. Food Chem.

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For decades, the tight regulatory functions of DNA and RNA have been the focus of extensive research with the goal of harnessing RNA molecules (e.g., microRNA and small interfering RNA) to control gene expression and to study biological functions. RNA interference (RNAi) has shown evidence of mediating gene expression, has been utilized to study functional genomics, and recently has potential in therapeutic agents. RNAi is a natural mechanism and a well-studied tool that can be used to silence specific genes. This method is also used in aquaculture as a research tool and to enhance immune responses. RNAi methods do have their limitations (e.g., immune triggering); efficient and easy-to-use RNAi methods for large-scale applications need further development. Despite these limitations, RNAi methods have been successfully used in aquaculture, in particular shrimp. This review discusses the uses of RNAi in aquaculture, such as immune- and production-related issues and the possible limitations that may hinder the application of RNAi in the aquaculture industry. Our challenge is to develop a highly potent in vivo RNAi delivery platform that could complete the desired action with minimal side effects and which can be applied on a large-scale with relatively little expense in the aquaculture industry.

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Section: Safety Of Rnai Usagementioning

confidence: 97%

Section: ■ Safety Of Rnai Usagementioning

confidence: 99%

RNA Interference in Aquaculture: A Small Tool for Big Potential

Abo‐Al‐Ela

2021

J. Agric. Food Chem.

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“…In addition, the commercial application of the antiviral dsRNA therapy in shrimp for human consumption may raise some safety concerns about introducing synthetic dsRNA molecules and pose a regulatory issue. A recent study by Rao et al [52] demonstrates that the dsRNA does not transfer to progeny from the broodstock females, indicating that the RNAi used as means of prevention before spawning should not pose a risk to human health.…”

Section: Therapeutic Approach: Viral Inhibition By Rna Interference (mentioning

confidence: 99%

Diversity of single-stranded DNA containing viruses in shrimp

et al. 2019

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Nanoparticles‐based double‐stranded RNA delivery as an antiviral agent in shrimp aquaculture

Jonjaroen,

Charoonnart,

Jitrakorn

et al. 2024

Reviews in Aquaculture

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The review encapsulates the comprehensive exploration of RNA interference technology's application in shrimp aquaculture, covering molecular intricacies, production methods, and practical applications. The emphasis is on exploring the stability and delivery of gene‐specific double‐stranded RNA, particularly through nanoencapsulation. This is because the susceptibility of dsRNA to degradation and limited cellular penetration, emphasising the need for effective delivery mechanisms. The review presents an in‐depth exploration of nanoparticles for the encapsulation and delivery of dsRNA, including virus‐like particles (VLPs), and non‐viral based nanoparticles such as liposomes, chitosan, and beta‐glucan. VLPs, derived from non‐infectious shrimp virus structures, exhibit biocompatibility and natural functionality, making them suitable carriers for dsRNA. The application of chitosan and its derivatives are explored for their efficacy in reducing viral infections in shrimp. Beta‐glucan particles are examined for their immunostimulant properties in shrimp aquaculture. The text emphasises the use of yeast‐based glucan particles for encapsulating dsRNA, showcasing their potential in preventing viral diseases in shrimp. The review introduces cautionary considerations for nanoparticle formulation, highlighting factors such as pH, organic solvents, metal ions, and environmental ribonuclease that can impact dsRNA stability during synthesis. In conclusion, the text discusses the potential economic worthiness and environmental risk assessment of dsRNA technology in shrimp aquaculture. While acknowledging successful applications in other environments, it underscores the need for regulatory approval and risk assessment for dsRNA‐based products in the aquaculture industry. The evaluation of dsRNA in real‐world shrimp farms is deemed necessary for commercial utilisation, taking into account income, expenses, safety, and environmental considerations.

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Double-stranded RNA injected into female Black Tiger shrimp (Penaeus monodon) prior to spawning does not transfer to progeny

Cited by 3 publications

References 46 publications

RNA Interference in Aquaculture: A Small Tool for Big Potential

RNA Interference in Aquaculture: A Small Tool for Big Potential

Diversity of single-stranded DNA containing viruses in shrimp

Nanoparticles‐based double‐stranded RNA delivery as an antiviral agent in shrimp aquaculture

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