Double strand RNA-mediated RNA interference through feeding in larval gypsy moth, Lymantria dispar (Lepidoptera: Erebidae)

Ghosh, Saikat; Gundersen‐Rindal, Dawn E.

doi:10.14411/eje.2017.022

Cited by 8 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, for a better understanding of the interactions between Cry toxins and APNs in Lepidoptera, it will be important to perform the functional characterization of some APN sequences found in this study. Successful attempts to identify the specificity of APN receptors to certain Cry toxins have been performed in the past, laying the basis for their biotechnological application [ 73 – 75 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

et al. 2020

View full text Add to dashboard Cite

The sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged in recent years as an alternative to currently applied methods. Genetic information about the target pests is often required to perform biotechnology-based management. The genomic and transcriptomic data for D. saccharalis are very limited. Herein, we report a tissue-specific transcriptome of D. saccharalis larvae and a differential expression analysis highlighting the physiological characteristics of this pest in response to two different diets: sugarcane and an artificial diet. Sequencing was performed on the Illumina HiSeq 2000 platform, and a de novo assembly was generated. A total of 27,626 protein-coding unigenes were identified, among which 1,934 sequences were differentially expressed between treatments. Processes such as defence, digestion, detoxification, signaling, and transport were highly represented among the differentially expressed genes (DEGs). Furthermore, seven aminopeptidase genes were identified as candidates to encode receptors of Cry proteins, which are toxins of Bacillus thuringiensis used to control lepidopteran pests. Since plantinsect interactions have produced a considerable number of adaptive responses in hosts and herbivorous insects, the success of phytophagous insects relies on their ability to overcome challenges such as the response to plant defences and the intake of nutrients. In this study, we identified metabolic pathways and specific genes involved in these processes. Thus, our data strongly contribute to the knowledge advancement of insect transcripts, which can be a source of target genes for pest management.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Challenges that affect successful silencing of gene expression include unique groups of dsRNA nucleases (dsRNases) expressed primarily in the gut tissue of insects and salivary secretions, or the gut pH which, may be responsible for degradation of dsRNA 47,77,78 . However, to overcome such degradation, systemic RNAi may be induced efficiently in insects by utilizing higher concentrations of dsRNA and/or using PEG in the diet for oral delivery of gene-specific dsRNA to overcome such degradation 62,79 . Delivery and uptake of dsRNA may also be stabilized with the help of carrier molecules, such as nanoparticles 80 like Chitosan .…”

Section: Discussionmentioning

confidence: 99%

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Ghosh

Hunter

Park

et al. 2018

JoVE

Self Cite

View full text Add to dashboard Cite

Phloem and plant sap feeding insects invade the integrity of crops and fruits to retrieve nutrients, in the process damaging food crops. Hemipteran insects account for a number of economically substantial pests of plants that cause damage to crops by feeding on phloem sap. The brown marmorated stink bug (BMSB), Halyomorpha halys (Heteroptera: Pentatomidae) and the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae) are hemipteran insect pests introduced in North America, where they are an invasive agricultural pest of high-value specialty, row, and staple crops and citrus fruits, as well as a nuisance pest when they aggregate indoors. Insecticide resistance in many species has led to the development of alternate methods of pest management strategies. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) is a gene silencing mechanism for functional genomic studies that has potential applications as a tool for the management of insect pests. Exogenously synthesized dsRNA or small interfering RNA (siRNA) can trigger highly efficient gene silencing through the degradation of endogenous RNA, which is homologous to that presented. Effective and environmental use of RNAi as molecular biopesticides for biocontrol of hemipteran insects requires the in vivo delivery of dsRNAs through feeding. Here we demonstrate methods for delivery of dsRNA to insects: loading of dsRNA into green beans by immersion, and absorbing of gene-specific dsRNA with oral delivery through ingestion. We have also outlined non-transgenic plant delivery approaches using foliar sprays, root drench, trunk injections as well as clay granules, all of which may be essential for sustained release of dsRNA. Efficient delivery by orally ingested dsRNA was confirmed as an effective dosage to induce a significant decrease in expression of targeted genes, such as juvenile hormone acid O-methyltransferase (JHAMT) and vitellogenin (Vg). These innovative methods represent strategies for delivery of dsRNA to use in crop protection and overcome environmental challenges for pest management.

show abstract

“…The uncharacterized genes of the gypsy moth were chosen to design the dsRNA using transcriptome sequencing data. Bacteria-expressing and in vitro synthesized dsRNA were provided to the pests through an artificial diet resulting in a reduction in body weight (Ghosh and Gundersen-Rindal 2017 ). In another study, the removal of the OCULAR ALBINISM TYPE 1 ( OA1 ) by injecting dsRNA into gypsy moth larvae showed an elevated 4.80-fold mortality compared to the control (Sun et al 2019a ).…”

Section: Dsrna Fungi- and Pesticide For Forest Insect Pests And Funga...mentioning

confidence: 99%

“…Respective pests and pathogens including target genes, methods, and final results are summarized. siRNA , small interfering RNA; dsRNA , double-stranded RNA; bp , base pairs Common name Scientific name Target gene Molecule and size Method Aim Final analysis Reference Insect pests European gypsy moth Lymantria dispar Ocular albinism type 1 (OA1) dsRNA & 200—650 bp Injection Insecticide 4.80-fold higher mortality Sun et al ( 2019b ) Olfactory receptor co-receptor (OrCo) dsRNA & 200 bp Injection Functional analysis Low pheromone response of male Lin et al ( 2015 ) Locus 365, Locus 28,365 dsRNA & 250 – 208 bp Feeding Insecticide 60% reduction in body mass Ghosh and Gundersen-Rindal ( 2017 ) Juvenile hormone epoxide hydrolase (JHEH) dsRNA & 513 bp Oral delivery Insecticide Delayed larval development Wen et al ( 2018 ) Ecdysone receptor and ultraspiracle (USP) dsRNA & 528 bp Injection Functional analysis Development shortened Wen et al ( 2020 ) Emerald ash borer Agrilus planipennis COP, IAP dsRNA & 247 – 272 bp Feeding Insec...…”

Section: Introductionmentioning

confidence: 99%

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Singewar¹,

Fladung²

2023

Funct Integr Genomics

View full text Add to dashboard Cite

Climate change alters the seasonal synchronization between plants and respective pests plus pathogens. The geographical infiltration helps to shift their hosts, resulting in novel outbreaks that damage forests and ecology. Traditional management schemes are unable to control such outbreaks, therefore unconventional and competitive governance is needed to manage forest pests and pathogens. RNA interference (RNAi) mediated double-stranded RNA (dsRNA) treatment method can be implemented to protect forest trees. Exogenous dsRNA triggers the RNAi-mediated gene silencing of a vital gene, and suspends protein production, resulting in the death of targeted pathogens and pests. The dsRNA treatment method is successful for many crop insects and fungi, however, studies of dsRNA against forest pests and pathogens are depleting. Pesticides and fungicides based on dsRNA could be used to combat pathogens that caused outbreaks in different parts of the world. Although the dsRNA has proved its potential, the crucial dilemma and risks including species-specific gene selection, and dsRNA delivery methods cannot be overlooked. Here, we summarized the major fungi pathogens and insect pests that have caused outbreaks, their genomic information, and studies on dsRNA fungi-and pesticides. Current challenges and opportunities in dsRNA target decision, delivery using nanoparticles, direct applications, and a new method using mycorrhiza for forest tree protection are discussed. The importance of affordable next-generation sequencing to minimize the impact on non-target species is discussed. We suggest that collaborative research among forest genomics and pathology institutes could develop necessary dsRNA strategies to protect forest tree species.

show abstract

Double strand RNA-mediated RNA interference through feeding in larval gypsy moth, Lymantria dispar (Lepidoptera: Erebidae)

Cited by 8 publications

References 45 publications

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Contact Info

Product

Resources

About