Double-strand Break Repair Machinery is Sensitive to UV Radiation

Ilnytskyy, Yaroslav; Yao, Youli; Kovalchuk, Igor

doi:10.1016/j.jmb.2004.11.001

Cited by 5 publications

(3 citation statements)

References 44 publications

(57 reference statements)

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“…Previously, we generated a transgenic Arabidopsis line that could monitor the level of point mutations in the plant genome . This line was successfully used in a number of mutagenesis assays, as well as in the analysis of the contribution of DSB repair to the generation of point mutations Ilnytskyy et al, 2005). The experiments in this study showed that point mutation rates increased slightly, although insignificantly, throughout the period between 2 and 31 dpg.…”

Section: Increased Mutation Ratementioning

confidence: 77%

Double-Strand Break Repair in Plants Is Developmentally Regulated

et al. 2006

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In this study, we analyzed double-strand break (DSB) repair in Arabidopsis (Arabidopsis thaliana) at various developmental stages. To analyze DSB repair, we used a homologous recombination (HR) and point mutation reversion assays based on nonfunctional b-glucuronidase reporter genes. Activation of the reporter gene through HR or point mutation reversion resulted in the appearance of blue sectors after histochemical staining. Scoring of these sectors at 3-d intervals from 2 to 31 d post germination (dpg) revealed that, although there was a 100-fold increase in the number of genomes per plant, the recombination frequency only increased 30-fold. This translates to a recombination rate at 31 dpg (2.77 3 10 28 ) being only 30% of the recombination rate at 2 dpg (9.14 3 10 28 ). Conversely, the mutation frequency increased nearly 180-fold, resulting in a 1.8-fold increase in mutation rate from 2 to 31 dpg. Additional analysis of DSBs over the early developmental stages revealed a substantial increase in the number of strand breaks per unit of DNA. Furthermore, RNA analysis of Ku70 and Rad51, two key enzymes in two different DSB repair pathways, and further protein analysis of Ku70 revealed an increase in Ku70 levels and a decrease of Rad51 levels in the developing plants. These data suggest that DSB repair mechanisms are developmentally regulated in Arabidopsis, whereby the proportion of breaks repaired via HR substantially decreases as the plants mature.

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Section: Increased Mutation Ratementioning

confidence: 77%

Double-Strand Break Repair in Plants Is Developmentally Regulated

et al. 2006

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“…17, 18 Signaling through free radicals may also play a crucial role in promoting changes in recombination frequency as mutants impaired in radical scavenging exhibit changes in recombination frequency. It is unlikely that ROS are gaseous signals that communicate genome instability.…”

Section: Discussionmentioning

confidence: 99%

Increase in recombination rate in Arabidopsis thaliana plants sharing gaseous environment with X-ray and UVC-irradiated plants depends on production of radicals

Zemp

Sidler²,

Kovalchuk³

2012

Plant Signaling & Behavior

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Keywords: X-ray, UV-C, DMSO, Arabidopsis thaliana, genomic instability, volatile signaling, homologous recombination rate, bystander effect X-ray and UVC are the two physical agents that damage DNA directly, with both agents capable of inducing doublestrand breaks. Some of our recent work has demonstrated that local exposure to UVC results in a systemic increase in recombination frequency, suggesting that information about exposure can be passed from damaged to non-damaged tissue. Indeed, we recently showed that plants sharing the same enclosed environment with UVC-irradiated plants exhibit similar increase in homologous recombination frequency as irradiated plants. Here, we further tested whether yet another DNA-damaging agent, X-ray, is capable of increasing recombination rate (RR) in neighboring plants grown in a Petri dish. To test this, we grew plants exposed to X-ray or UVC irradiation in an enclosed environment next to nonexposed plants. We found that both X-ray and UVC-irradiated plants and neighboring plants exhibited comparable increases in the levels of strand breaks and the RR. We further showed that pre-exposure of plants to radical scavenger DMSO substantially alleviates the radiation-induced increase in RR and prevents formation of bystander signal. Our results suggest that the increase in RR in bystander plants can also be triggered by X-ray and that radicals may play some role in initiation or maintenance of this signal.

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“…A exposição das moléculas de DNA a essas radiações pode levar à formação de lesões singulares ou múltiplas, tais como danos nas bases ou açúcares, sítios álcali-lábeis, quebras de fita única (SSBs) e quebras de fitas duplas (DSBs), sendo esta última considerada a lesão mais deletéria para as células in vivo ou in vitro (KARANJAWALA et al, 2002). Entre todas as lesões causadas por radiação UV, as quebras do DNA são consideradas as mais perigosas já que desintegram sua estrutura linear (ILNYTSKYY et al, 2005). O DNA danificado e não reparado pode acarretar em defeitos na transcrição e replicação da molécula, resultando em uma série de transtornos, incluindo a morte celular, câncer e mudanças hereditárias via mutagênese (KORNHAUSER et al, 1991 (HÄKKINEN et al, 2004).…”

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Avaliação da fototoxicidade do antraceno sobre a mortalidade e o dano ao DNA de juvenis de pampos, Trachinotus carolinus (Linnaeus, 1766)

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Double-strand Break Repair Machinery is Sensitive to UV Radiation

Cited by 5 publications

References 44 publications

Double-Strand Break Repair in Plants Is Developmentally Regulated

Double-Strand Break Repair in Plants Is Developmentally Regulated

Increase in recombination rate in Arabidopsis thaliana plants sharing gaseous environment with X-ray and UVC-irradiated plants depends on production of radicals

Avaliação da fototoxicidade do antraceno sobre a mortalidade e o dano ao DNA de juvenis de pampos, Trachinotus carolinus (Linnaeus, 1766)

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