2012
DOI: 10.1016/j.bios.2012.01.025
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Double-receptor sandwich supramolecule sensing method for the determination of ATP based on uranyl–salophen complex and aptamer

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Cited by 37 publications
(20 citation statements)
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“…ATP is also a molecule containing phosphate group. In recent years, several sandwich type methods for the determination of ATP by utilizing the formation of supramolecule ATP-uranyl-salophen have been developed and have their advantages [32,33]. However, in these sandwich type methods a separation procedure is necessary.…”
Section: Introductionmentioning
confidence: 99%
“…ATP is also a molecule containing phosphate group. In recent years, several sandwich type methods for the determination of ATP by utilizing the formation of supramolecule ATP-uranyl-salophen have been developed and have their advantages [32,33]. However, in these sandwich type methods a separation procedure is necessary.…”
Section: Introductionmentioning
confidence: 99%
“…They are isolated from a synthetic nucleic acid pool by Systematic Evolution of Ligands by EXponential enrichment (SELEX) [1][2][3]. Aptamers rival antibodies in terms of stability, specificity and affinity toward a wide range of target molecules such as protein, small molecules, amino acids and even cells [4][5][6].…”
Section: Introductionmentioning
confidence: 99%
“…2.2.1 Synthesis of the uranyl-salophen complex. 32 4-Chloromethyl-o-phenylenediamine (1.50 g) and 2-hydroxy-5chloromethyl benzaldehyde (3.40 g) were dissolved in 80 mL of anhydrous ethanol in a 250 mL three-necked ask. The mixture was stirred for 2 h under reux and then cooled to room temperature.…”
Section: Preparation Of Umcamentioning
confidence: 99%
“…The solid mass was ltered, and the product, salophen, was recrystallized from ethanol and vacuum-dried for 2 h. Next, this salophen was added into a beaker containing 50 mL ethanol, and 3.75 g hexahydrate uranyl nitrate was added to the mixture, which was then stirred for 45 min under reux and cooled to room temperature. Aer ltering and washing with ethanol three times, the synthetic product, uranyl-salophen complex, was dried under vacuum at 60 C for 12 h. The synthesis process and structure 17,19,20,32 of the uranyl-salophen complex is illustrated in Scheme 1.…”
Section: Preparation Of Umcamentioning
confidence: 99%