Double-phase culture system for large scale production of pineapple

Scherwinski‐Pereira, Jonny Everson; Lima, Elequisandra da C. Araruna; Silva, Tatiane L. da; Mesquita, A. G. G.; Maciel, Simone de Alencar; Costa, Frederico Henrique da Silva

doi:10.1007/s11240-011-0091-8

Cited by 23 publications

(21 citation statements)

References 25 publications

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“…Cost analysis Table 2: A comparative study on micropropagation of Rauwolfia serpentine in two different culture systems after 6 weeks of culture [5,4] in DPS where both the phase contains the same medium. In the dual phasic culture system as reported earlier they have shown the increased shoot multiplication and growth only at a time in the same culture vessel, where as the current system along with the increased shoot growth it has also shown a good rooting response in the same culture vessel.…”

Section: Methodsmentioning

confidence: 99%

“…of plants can be propagated. To date a very few reports on DPS has been reported that has improved the efficiency of in vitro shoot development in certain plants like in Pineapple [4], in conifers [5] and in Japanese pear [6]. All these DPS study, reported same media composition (both in the semi-solid and liquid phase) responsible for increasing the efficiency of shooting only.…”

Section: Saj Biotechnology Issn: 2375-6713mentioning

confidence: 99%

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A Double Phase Culture System: An Economic and Time Saving Protocol for in vitro Propagation of Plant

Senapati¹

2015

Scholarena Journal of Biotechnology

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Indiscriminate over exploitation from natural source to meet the growing demand by industry coupled with low seed viability, lack of vegetative propagation methods and insufficient attempts for replenishment of wild stock of different important plant species have contributed to its threatened status. So realizing the threat of extinction and to meet the growing needs, the alternate method for propagation is micropropagation. This technique is used not only for those plants which are difficult to be propagated through conventional practices, but also for the mass multiplication of existing stocks of germplasm for biomass production and conservation of important, elite and rare plant species that are threatened or on the verge of extinction [1]. In all reports, protocols described are based on conventional micropropagation system (CMS). This CMS explained most common methods of micropropagation (Table 1), which involve initiation/bud proliferation, the shoot multiplication followed by rooting via a semi solid medium. Although CMS have been highly successful in terms of multiplication yields, it has become increasingly important to improve productivity and uniformity of commercially important material in reduced cost, time and space [2]. Hence an improvement in plant tissue culture techniques for the mass propagation of commercial plants is highly desirable [3]. An alternative that has shown potential to improve the efficiency of protocols for in vitro multiplication is the use of a double-phasic culture system (DPS). In this method, the explants remain in the semi-solid medium and the liquid medium is added over periodically throughout the culture, thus Low cost and time saving tissue culture techniques by improving process efficiency and better utilization of resources without compromising the quality of the plants are of high priority for commercial utilization. In this report a double phase culture system (DPS) i.e. a semisolid phase with rooting media at the bottom with a layer of shooting media in liquid phase, was standardized and compared with conventional micro propagation system (CMS) by taking Rauwolfia serpentine L. Bent. as a model. In CMS the shoots were raised first followed by two subsequent subculture then they were transferred to rooting media for root induction for which it will take more than 12 weeks but under DPS, plantlets with well developed roots and shoots can be produced simultaneously in a single culture vessel within 08 weeks without shoot manipulations/repeated subculture. It was found that the rate of multiplication (98.7), shoot length (9.8) and shoot thickness (5.2) was higher in DPS in comparison to CMS. The no. of roots/shoot (8.7), root length (5.3cm), root thickness (6.1mm) was found higher in DPS than that of the CMS. Percentage survival of plantlets was 98.5 % in DPS, while it was only 70% in CMS. The culture period was reduced by 44% in DPS compared to the CMS. In DPS nutrient cost/plantlet, energy cost/plantlet and labour cost also reduced by 35.36%, 40.66% and 33.33% respe...

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Section: Methodsmentioning

confidence: 99%

Section: Saj Biotechnology Issn: 2375-6713mentioning

confidence: 99%

A Double Phase Culture System: An Economic and Time Saving Protocol for in vitro Propagation of Plant

Senapati¹

2015

Scholarena Journal of Biotechnology

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“…Penggunaan media dua-lapis (double layer) yaitu penambahan media cair di atas media padat mampu memadukan kelebihan media padat dan media cair. Media dua-lapis telah banyak digunakan untuk optimasi pertumbuhan berbagai tanaman in vitro (de Oliveira, 2011;Scherwinski-Pereira et al, 2012;de Oliveira et al, 2013;Gatambia et al, 2016).…”

Section: Pendahuluanunclassified

Modifikasi sistem kultur in vitro untuk meningkatkan vigor planlet stevia (Stevia rebaudiana Bert.)

Saptari¹,

SUMARYONO²

2017

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Stevia (Stevia rebaudiana Bert.), a sweetener plant, has been mass propagated by tissue culture technique. Optimal conditions to increase vigor of stevia plantlets are needed to support the sustainability of in vitro plantlet stocks and increase plantlet survival rate during acclimatization. The aim of this research was to investigate the effect of different media, culture vessel sizes, and vessel closure types on the vigor of stevia plantlets. The plant material was derived from apical shoot cuttings of sterile stevia plantlets grown on WP medium without growth regulator. Several treatments used in this study were solid or double layer media; short or tall culture vessel; and polypropile screw cap or plastic film closures. Growth of plantlets was determined after 3 weeks of culture. Temperature and light intensity inside the vessels were also observed. The results showed that the best treatment to increase the vigor of stevia plantlets was a double-layer medium in a tall culture vessel (diameter 7 cm and height 11 cm) with either screw cap or plastic film. It was exhibited by significantly bigger stem diameter, more and bigger leaves, longer roots, and higher biomass fresh weight than those of other treatments. Higher temperature was observed on tall culture vessel, whereas all treatments did not significantly affect light intensity inside the vessels.[Keywords: stevia, plantlet vigor, double-layer medium, culture vessel size, vessel closure]AbstrakStevia (Stevia rebaudiana Bert.), tanaman pemanis, telah diperbanyak melalui teknologi kultur jaringan. Kondisi kultur optimal untuk meningkatkan vigor planlet stevia masih diperlukan untuk mendukung keberlanjutan tanaman stock in vitro dan untuk meningkatkan daya hidup planlet ketika diaklimatisasi. Penelitian yang dilakukan bertujuan untuk menentukan pengaruh penggunaan jenis media, ukuran botol kultur, dan jenis penutup botol yang berbeda terhadap vigor planlet stevia. Material tanaman yang digunakan didapat dari potongan tunas apikal plantlet stevia steril yang ditumbuhkan pada media WP tanpa zat pengatur tumbuh. Perlakuan jenis media terdiri atas media padat dan media dua-lapis (double-layer), ukuran botol pendek dan tinggi, serta jenis tutup ulir berbahan polipropilen dan lembaran plastik transparan. Pengamatan pertumbuhan planlet dilakukan setelah 3 minggu, juga dilakukan pengamatan terhadap suhu dan intensitas cahaya di dalam botol kultur. Hasil penelitian memperlihatkan bahwa perlakuan terbaik untuk meningkatkan vigor planlet stevia adalah dengan menggunakan media dua-lapis dalam botol kultur (diameter 7 cm, dan tinggi 11 cm), baik dengan tutup ulir maupun plastik. Hal ini ditunjukkan dari diameter batang lebih besar, daun lebih banyak dan besar, akar lebih panjang, serta bobot segar biomassa lebih tinggi dibandingkan perlakuan lainnya. Suhu lebih tinggi terukur pada perlakuan botol tinggi, sedangkan semua perlakuan tidak mempengaruhi secara nyata intensitas cahaya di dalam botol kultur.[Kata kunci: stevia, vigor, media dua-lapis, ukuran botol kultur, tutup botol]

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“…Other factors influencing plant recovery include the genotype, the physiological state of the explants, and the culture conditions. Furthermore, the use of an appropriate culture medium for each stage is a basic condition for the success of the process and is responsible for providing the nutrients necessary for the metabolism of the developing plant cells (Scherwinski-Pereira et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The majority of work on plant micropropagation is based on the use of semi-solid culture media. However, the use of liquid, double-phase nutrient media has provided equal or even greater efficiency for many plant species (Zamzuri 1998;Tarmizi et al 2008;Steinmacher et al 2011;Scherwinski-Pereira et al 2012;Oliveira et al 2013). Furthermore, this system features ease of the preparation and handling of the media, the reduced production costs provided by the elimination or reduction of gelling agent, and the possibility of using a small quantities of culture medium.…”

Section: Introductionmentioning

confidence: 99%

Optimizing rooting and survival of oil palm (Elaeis guineensis) plantlets derived from somatic embryos

Gomes

Bartos

Scherwinski‐Pereira

2015

In Vitro Cell.Dev.Biol.-Plant

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In species of the Arecaceae, initial conversion of somatic embryos to non-rooted plantlets (those with only shoot development and no roots) is common. A consistent methodology for improving rooting and survival of oil palm plantlets derived from somatic embryos was developed. Two experiments were carried out. In the first, non-rooted shoots regenerated via somatic embryogenesis were inoculated in nutrient medium supplemented with 0, 53.7, or 107.4 μM indole-3-butyric acid (IBA) or 1-naphthaleneacetic acid (NAA). In the second, the optimum treatment as determined in the first experiment was evaluated in three culture systems: semi-solid medium (SS), stationary liquid (SL), and doublephase system (DP), with and without the addition of activated charcoal. Next, the plantlets were acclimatized in a greenhouse, where the influence of the presence or absence of roots, the number of roots, the length of the main root, and the height of the shoot on survival were assessed. Supplementation with 53.7 μM IBA and the DP system without activated charcoal provided the highest percentage of rooted plantlets (92.9%) and number of roots (6.3) and improved the length of the aerial parts (11.0 cm) and the survival of the plantlets during the acclimatization (82.1%). The best survival of plantlets was obtained when they were acclimatized with at least one root and with a height greater than 7.5 cm.

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Double-phase culture system for large scale production of pineapple

Cited by 23 publications

References 25 publications

A Double Phase Culture System: An Economic and Time Saving Protocol for in vitro Propagation of Plant

A Double Phase Culture System: An Economic and Time Saving Protocol for in vitro Propagation of Plant

Modifikasi sistem kultur in vitro untuk meningkatkan vigor planlet stevia (Stevia rebaudiana Bert.)

Optimizing rooting and survival of oil palm (Elaeis guineensis) plantlets derived from somatic embryos

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