Double NCED isozymes control ABA biosynthesis for ripening and senescent regulation in peach fruits

Wang, Pengfei; Lu, Siyuan; Zhang, Xueying; Hyden, Brennan; Qin, Lijie; Liu, Lipeng; Bai, Yangyang; Yan, Han; Zhuang, Wen; Xu, Jin; Cao, Hongrui; Chen, Haijiang

doi:10.1016/j.plantsci.2020.110739

Cited by 38 publications

(14 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on our results, the preharvest application of COS exhibited an overall decrease in the decay incidence in the blackberries, which can be attributed to the inhibition of the PG enzyme and consequently, a reduction in the degradation of cell structures, and the overall antifungal properties of COS (Nia et al, 2021;Wu et al, 2021). Similarly, the impact of preharvest applications of SA on maintaining the MI of the blackberry fruit could be attributed to the reduction in the fruit softening processes and the delay of the ripening by the antagonist effect of SA with senescence-related phytohormones (García-Pastor et al, 2020b;Gomes et al, 2021;Wang et al, 2021). Even COS and SA showed similar results on the blackberry MI preservation, different mechanisms could be in action for each case.…”

Section: Discussionmentioning

confidence: 99%

Delayed Senescence and Marketability Index Preservation of Blackberry Fruit by Preharvest Application of Chitosan and Salicylic Acid

et al. 2022

View full text Add to dashboard Cite

Blackberry fruits are appreciated as a source of nutrients and compounds related to benefit human health. However, they are highly perishable and very susceptible to decay factors. Current methods to improve and maintain blackberry quality are limited in use because of the fruit's fragile physical properties. Regarding these properties, it has been reported that the activities of certain enzymes are linked to senescence and fruit softening processes. This study was aimed to assess the effect of salicylic acid (SA) and chitosan (COS) as preharvest treatments on the physiology related to improving fruit conservation and preserving the marketability index of blackberry fruit. The preharvest treatments were foliar sprayed on blackberry plants at different concentrations. The activities of enzymes superoxide dismutase (SOD), catalase (CAT), phenylalanine ammonia-lyase (PAL), and polygalacturonase (PG) were measured. Total soluble solids (TSS), titratable acidity (TA), TSS/TA ratio, and marketability index (MI) were analyzed after 144 h of storage. The application of 3 mM of SA and 0.25% of COS treatments preserved the MI of blackberries by reducing leakage, red drupelet reversion (RDR), and mycelium presence in the fruit. SA application increased SOD, CAT, and PAL activities. Our results also showed that SA and COS preharvest treatments modified the activity of the cell wall degrading enzyme PG, which might play a role in improving the shelf life and resistance to decay factors of blackberry fruit without any significant effects on physicochemical properties like TSS, TA, and the TSS/TA ratio.

show abstract

Section: Discussionmentioning

confidence: 99%

Delayed Senescence and Marketability Index Preservation of Blackberry Fruit by Preharvest Application of Chitosan and Salicylic Acid

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Many investigations have focused on the effects of ABA on the fruit development and ripening, especially in non-climacteric fruit species. It had been demonstrated that exogenous ABA treatment significantly enhanced the accumulation of sugars (Alferez et al, 2021), double NCED isozymes controlled ABA biosynthesis for ripening and senescent regulation in peach fruits (Wang et al, 2021). Furthermore, in climacteric fruit species, it had been suggested that overexpression of the abscisic acid β−glucosidase gene (DkBG1) altered fruit ripening in transgenic tomato (Liang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Exogenous Abscisic Acid Mediates Berry Quality Improvement by Altered Endogenous Plant Hormones Level in “Ruiduhongyu” Grapevine

Liu

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Abscisic acid (ABA) plays a key role in fruit development and ripening in non-climacteric fruit. A variety of metabolites such as sugars, anthocyanins, fatty acids, and several antioxidants, which are regulated by various phytohormones, are important components of fruit quality in grape. Here, grape cultivar “Ruiduhongyu” was used to investigate the relationship between endogenous phytohormones and metabolites associated to grape berry quality under exogenous ABA treatment. 500 mg/L ABA significantly improved the appearance parameters and the content of many metabolites including sugar, anthocyanin, and other compounds. Exogenous ABA also increased the contents of ABA, auxin (IAA), and cytokinins (CTKs), and transcription level of ABA biosynthesis and signaling related genes in fruit. Furthermore, a series of genes involved in biosynthesis and the metabolite pathway of sugars, anthocyanins, and fatty acids were shown to be significantly up-regulated under 500 mg/L ABA treatment. In addition, Pearson correlation analysis demonstrated that there existed relatively strong cooperativities in the ABA/kinetin (KT)-appearance parameters, ABA/IAA/KT-sugars, ABA/indolepopionic acid (IPA)/zeatin riboside (ZR)-anthocyanins, and gibberellin 3 (GA3)/methyl jasmonate (MeJA)-fatty acids, indicating that 13 kinds of endogenous phytohormones induced by ABA had different contributions to the accumulation of quality-related metabolites, while all of them were involved in regulating the overall improvement of grape fruit quality. These results laid a primary foundation for better understanding that exogenous ABA improves fruit quality by mediating the endogenous phytohormones level in grape.

show abstract

“…ABA stimulates several changes in plant physiological, molecular and developmental progressions, resulting in plant adaptation to the stress environment, and it is characterized as an intracellular messenger that has been suggested to perform critical functions as the core of signalling operations [36,37]. In our study, the expression of the two ZEP genes related to ABA biosynthesis was strongly increased in the H1 stage, while the NCED [38,39] gene was downregulated in the LS6 strain. This result indicates that the genes related to ABA biosynthesis in HZ88 show an upwardregulated expression trend as a whole, which may increase the level of ABA in HZ88.…”

Section: Discussionmentioning

confidence: 46%

Use of Comparative Transcriptomic Analysis of Different Potato Varieties to Elucidate the Molecular Mechanism Underlying Differences in Cold Resistance

Yang¹,

Chen²,

Kou³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Among the various abiotic stresses, cold is an essential factor that limits crop productivity worldwide. Low temperature affects the growth, development and distribution of agronomic species around the world. To improve the understanding of the physiological and genetic properties and functions affecting potato cold tolerance, in this study, transcriptomic analysis was performed on two potato strains (HZ88 and LS6) with different cold tolerances that were treated at low temperature for 0, 1, 3, and 6 hours. Results Transcriptomic analysis showed that there were large differences between HZ88 and LS6 regarding the expression levels of low-temperature response genes. Notably, HZ88 responds to low-temperature stress, its low-temperature response genes are primarily enriched in plant hormone signal transduction; cutin, suberine and wax biosynthesis; and photosynthesis-antenna proteins. Conversely, the most significant low-temperature response genes of the LS6 strain were determined to be enriched in plant-pathogen interactions, zeatin biosynthesis, and plant hormone signal transduction. The cuticle, composed of a horny waxy layer, is an important protective barrier formed by plants to resist biotic/abiotic stress during the long-term ecological adaptation process, and the HZ88 strain may strengthen its cold resistance by enhancing this physical defence measure. In the LS6 strain, potatoes tend to cope with cold stress by strengthening their immune system and regulating hormone signal transduction. In addition, hormone pathway-related genes (such as ABA), ICE-CBF signalling pathway-related genes, and genes encoding TFs all exhibited different expression patterns between HZ88 and LS6. Conclusions To the best of our knowledge, this study is the first to elucidate the genetic mechanisms underlying the difference in cold resistance between the strongly cold-tolerant variety LS6 and the weakly cold-tolerant variety HZ88, thereby establishing a foundation for further analysis and genetic breeding.

show abstract

Double NCED isozymes control ABA biosynthesis for ripening and senescent regulation in peach fruits

Cited by 38 publications

References 59 publications

Delayed Senescence and Marketability Index Preservation of Blackberry Fruit by Preharvest Application of Chitosan and Salicylic Acid

Delayed Senescence and Marketability Index Preservation of Blackberry Fruit by Preharvest Application of Chitosan and Salicylic Acid

Exogenous Abscisic Acid Mediates Berry Quality Improvement by Altered Endogenous Plant Hormones Level in “Ruiduhongyu” Grapevine

Use of Comparative Transcriptomic Analysis of Different Potato Varieties to Elucidate the Molecular Mechanism Underlying Differences in Cold Resistance

Contact Info

Product

Resources

About