Double-layered Nanofibrous Patch for Prevention of Anastomotic Leakage and Peritoneal Adhesions, Experimental Study

Using virtual microscopy for the development of sampling strategies in quantitative histology and design‐based stereology

“…Example #5 demonstrates three different sampling densities used for quantification of three parameters when evaluating the healing of intestinal anastomoses. The sampling was facilitated using Stereologer software (Rosendorf et al, 2020, 2021a, 2021b). (a) Scanned slide shows the porcine small intestine.…”

Design and fabrication of modified bi-layer poly vinyl alcohol adhesive sealant film for preventing gastrointestinal leakage

“…demonstrated that applying a double-layered polycaprolactone and polyvinyl alcohol nanofibrous promotes anastomosis healing in animal models. Similar to our study, there was no AL in both control and case groups, and because of that, they could not assess the role of double-layered nanofibrous in preventing AL ( 23 ).…”

“…Due to the significant burdens of anastomosis leakage on patients and health care providers, different methods have been introduced to overcome this problem. One of the popular methods among clinicians is the application of sealants and adhesives ( 15 – 17 ) including fibrin glue with or without additional ( 18 – 20 ) cyanoacrylates adhesives ( 21 , 22 ) and polyethylene glycol-based adhesives ( 23 ). Despite the popularity of bio-glues, several drawbacks, including inhibiting wound healing, decreasing anastomotic strength, limiting macrophage migration, neutrophil function, lack of long durability, high cost, and inaccessibility, have been demonstrated ( 24 , 25 ).…”

“…The results revealed adhesive energy of about 120 with perfect mechanical integrity maintenance and good anastomosis leakage prevention ( 28 ). Rosendorf et al developed a double-layer nanofibrous patch based on PCL/PVA utilizing a needleless electrospinning device for anastomosis leakage prevention resulting in providing a good adhesion with no completion ( 23 ). The double-layer bioadhesive gastrointestinal film fabricated by Wu et al also revealed ideal mechanical properties as well as desirable adhesion prospects ( 29 ).…”

Advances in Hydrogel Adhesives for Gastrointestinal Wound Closure and Repair

“…Bioactive and porous three-dimensional hydrogel constructs enhance regeneration of the sutured site by providing a mechanically stable and hydrophilic environment conducive to cell attachment, proliferation, and differentiation [ 78 ]. An additional hydrogel design is the introduction of an anti-adhesive backing layer to prevent post-operative peritoneal adhesion, which causes abdominal discomfort or even more serious complications such as ileus, necessitating reoperation [ 79 ].…”